LOCUS       BBa_K1256007            2422 bp    DNA     linear       10-Dec-2025
DEFINITION  pSB1C3-Plac-SS-Lipase
ACCESSION   BBa_K1256007
VERSION     BBa_K1256007.1  
FEATURES             Location/Qualifiers
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..220
                     /label=BBa_B0034
     CDS             296..2416
                     /label=lipase
     misc_feature    2417..2422
                     /label=Scar (NsiI + PstI)
     CDS             227..289
                     /label=OmpA secretion signal
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aaaaaacggc
      241 cattgctatc gctgtcgcgt tggcgggctt cgcgactgta gctcaggcgg ctagcatgag
      301 tgagcccgtg accgatatcg gccgacgccg gttgttgcag gccggcgttg ccgccggcct
      361 tgccccgctg ctgcccagca tcgcccgggc cgcagcgatc gcccccgccg cgcagacgcg
      421 cagcctcgaa gacctgcagc acatcgtggt gttcatgcag gagaaccgtt cgttcgacca
      481 ctacttcggc acgctgccgg gcgtacgtgg tttcggtgac cgcttcgtgg ccccggccgc
      541 ctcgctgcag gccggcggcc gcgcgcgcag cctgtggctg cagcccgatg ccagtggcac
      601 gcgggcgatc gcaccgttcc cgctggatac cgccgcgcac ttcggctaca tgcgggtgga
      661 aggcacgccg cacacctggg cagacgccca gcgtgcctgg gaccacggcc gcatggggca
      721 ctggccgaag gccaagcaga accactccat gggctacttc cagcgcagcg acatgccgtt
      781 ccagttcgcg ctggccgatg cgttcacgat ctgcgacgcc taccactgcg cgatgcaggc
      841 cggcaccaat ccgaaccggg tgttcctgtg gaccggccac aacgatgcac gggcccgcgc
      901 cggcggcccg gtgatcgcca actcgcatga caacttcccc gaacacggcg ggcatccggc
      961 gtcctaccgc tggaccagct atgtcgagcg cctgcagaag gccggcgtgt cctggcagat
     1021 ctaccaggac atggcggaca acttcaccga caacccgctg gccggcttcg aggcgttccg
     1081 tcgggcgtac agtgctgcac ccgggcatga cccgcaactg cgcgcacgcg gggtcagcac
     1141 acgtggcctg ccacagctac gccaggacgt actggccggg cgcctgccgc aggtcagctt
     1201 catcatcgcc gacgcggccg gcagcgagca ccccggaccc tccagtccgg cgcagggcgc
     1261 ggcctatacc gcgcgcgtgc tggatgcgct gactgccgat ccggaagtct ggagccgcac
     1321 cgccctgctg ctgatgttcg atgagaacga cggcttcttc gaccacatgc cgccaccggc
     1381 accgccttcg ccggtggatg gcggctgggc gggtgcgtcc tcggtcagta ccgagggcga
     1441 ataccaccgc catcccgcac cgggcgacga gaagtacgat gccgccgaac tgcgcggccg
     1501 cccgtatgga ctgggcccgc gcgtgccgct gtatgtgatc tcgccgtgga gtcggggtgg
     1561 ctgggtcgac tcgcaggtct acgaccacac ctcggtcatt cgactgatcg aacgccgctt
     1621 cggtgtggcg gcaccggaca tctcgccgtg gcgccgcgcg gtctgcggtg acctgacggg
     1681 tgccttcgac ttcgcccagc gcgatacccg cccgttcgta cgcggcctgc ccgacgtgag
     1741 cgcggtggcc gcacgcgccg ctgcgttgcc agggcgaacc gtgccggcgc tgccggaggg
     1801 gctgaaacca gcgaagcagg aagtcggcgt acgccctgca cgcgccctgc cctatcggcc
     1861 gcaggcatcg atggctgcgg tcgacgcctc gggcgtggat ctggcgctgg cctgcgaagg
     1921 tgcggcggcg gtgctgcatg tctacgaccg gctgcggctg gatgcggtgc cacgccgcta
     1981 cacgctgctg ccgggcatgg cgctgcgcga gcgctggccg ctcgatgccc aggaccgcta
     2041 tgacctgtgg ctgctgggcc cgaacggctt ccaccgccac ttccaggggg cggccaagga
     2101 tcctgcgctg caggcttcaa ttgatcgcac ggatggccaa ctgcggctgc ggctgcgcaa
     2161 cctgggtgac ggcgcgcagc aggtgcgtgt gcagcctggt gcctatgccg ggcacatgcc
     2221 cgatcaggcg ctggtactac ccgcacacgc agaaaccgcg ttggcgtggg acgcaacgcc
     2281 cacggcgggc tggtatgacc tgcagatcgg cgcgggcgag agcatgctgc gcctggccgg
     2341 tcgcgccgag gatgggcgcc ccggcacgag tgacccggcg atgggcagcg aaccactgcg
     2401 gttcgagcag gattgaatgc ag
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