LOCUS       BBa_K1256009            3003 bp    DNA     linear       22-Nov-2024
DEFINITION  pSB1C3-Plac-SS-RNase
ACCESSION   BBa_K1256009
VERSION     BBa_K1256009.1  
FEATURES             Location/Qualifiers
     promoter        1..200
                     /label=BBa_R0010
     stem_loop       2875..3003
                     /label=BBa_B0015 
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..289
                     /label=OmpA secretion signal
     CDS             296..2860
                     /label=RNase
     misc_feature    2861..2866
                     /label=Scar (Bgl II + BamHI)
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aaaaaacggc
      241 cattgctatc gctgtcgcgt tggcgggctt cgcgactgta gctcaggcgg ctagcatgaa
      301 tccgcaggct gtcaacgact tcctgaatat ttttcacgca aggccttcaa gacggccact
      361 gacccgttct gcacgccgtc aacggctacc atgtagggcg atgactacca aaaaaccaag
      421 caagggcggg agcacctccc gcagccaggc cccgaagaag ggcgccaagg cgggcacggc
      481 ccgcgccacc aagccgggca agccgttgcc gggctggttc cctgaattga atgaaggcgg
      541 cgcaccgccg cgtggccgca aggtccgcag cagcgacgca ccgggcccgg ccccgggccg
      601 caagttgccg ccgaccggca aggtgatcga cgatccctat gccgcccgcg aagcagagaa
      661 gtacgaacag cccatcgcga gccgcgaagc catcctggcc ctgctggagc gttgcgaagg
      721 gccgcagacg gcagaggaac tgggcgcacg cctgggcctg accgccccgg accgtgccga
      781 agcactgtcg cgccggctcg gcgccatggt ccgcgacggc cagctggtgc agaaccgccg
      841 cggcggcttc gccccgatcc agaccctgaa cctggtcacc ggcgtggtga tcgccaaccc
      901 ggaggggttc ggcttcctgc gcccggtcga gggcggcgac gacctgttcc tgccgccgta
      961 tgaaatgcgc aaggtcatgc acggcgacaa ggtgctggcc cgcgtgaccg gcatcgacca
     1021 ccgtggccgc cgcgaaggca gcatcgcccg cgtgctggag cgcggcatga cccgcctgat
     1081 cggtcgcttc agcatcgaga tgggcatcaa ctacgtggtg cccgacgaca agcgcgtgca
     1141 gcgcaacgtg caggtgccgc cggaccagac cggtggtgcg cgcgacggcc agttggtggt
     1201 ctgcgaactg acccaggcgc cggacagccg tcgcccgccg atcggccgca tcatcgccgt
     1261 gctcggcgac aagctgaccg cttcgctggt ggtggagacc gccatccacg gccatgaact
     1321 gccattcgag ttcccgcagg aagtgctgga cgaagccgcc tcggtgccgc tggtggttga
     1381 gccggcgatg atcggcgacc gcgtggacct gcgcagcatg ccgctggtca ccatcgatgg
     1441 cgaggatgcc aaggacttcg acgacgcggt gtactgcgag ccgaatgccg atggcttccg
     1501 gctggtggtg gcgattgccg atgtctccaa ctacgtccgg cccggcacgc cactggacga
     1561 agaggcgcag aagcgtgcca cctcggtgta cttcccgggc ttcgtggtgc cgatgctgcc
     1621 ggagacgctc tccaacggca tctgttcgct gatgccgaag gtcgaccgca tgtgcttcgt
     1681 ctgcgacatg cagatcgacc gcgacggtct ggtcacgcat tcgcgcttct acgaagcggt
     1741 gatgaactcg catgcgcgcc tgacctacac ccaggtgtgg aaggcggtgg gtgaggacga
     1801 tgccgatacc aaggcctgga tgggcgacct gctgccgcag gtgcagcgcc tgcaccagct
     1861 gtacaaggtc ctgtccaagg cgcgtgccaa gcgtggcgcc atcgagttcg aaagcagcga
     1921 agtgcgcttc gtgctggaca accgcggtga agtgacccag gccggcatgc tggtgcgcaa
     1981 tgacgcgcac aagctgatcg aggaatgcat gatcgcggcc aatgtggagg cggcgaagta
     2041 cctgctgtcg cgccatgtgc cggccccgta ccgcatccac gagaagccgc cggaaaccaa
     2101 gtacgccgac ctgctggaat tcctcaagga gttcaagctg agcctgccgc cgtggtcgaa
     2161 ggtgcgcccg ggcgattaca ccaagctgct gaagaagatc cgcgaccgcc ccgatgccac
     2221 gctgctggaa tcggtgctgc tgcgcagcca gagtctggcg atctacagcc cggaaaacca
     2281 cggtcacttc ggcctggcgc tggaggcgta cgcgcacttc acctcgccga tccgccgtta
     2341 ccccgacctg ctggtgcacc gcgcgatcaa gcacgcgctg tccggcaagc cgctggacaa
     2401 gttcacctac aacgcgcgcg aaatggccgc gctggcgctg cagtgctccg agcgcgagcg
     2461 ccgtgccgat gaagccgagc gcgaggtcga cgagcgttac cgcgcggcgt ggatggaaaa
     2521 gcacgtgggt ggccagttcg atggcgtgat cagcggcgtg accagcttcg gcctgttcgt
     2581 ggagctggac gagtcgaagg tgcagggcct ggtgcacgtg acccagctgc cgcaggacta
     2641 ttacaagttc gacgccacgc gcaagacgct gaccggcgag cgccgcggca gcagctaccg
     2701 gctgggtgac cgcgtgcgga tcctggtact gaaggccagc atggaagagc gcaagatcga
     2761 cttccgcctg gtcgaacaca agggcgagga cgaaggcgat ggcctgccgc cgctgccgga
     2821 gcgtggcaag ccggccaagc ggaagaaaga gaaatactga agatcctact agagccaggc
     2881 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     2941 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     3001 ata
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