LOCUS       BBa_K1256010            2401 bp    DNA     linear       31-Dec-2025
DEFINITION  pSB1C3-Plac-SS-Chitinase
ACCESSION   BBa_K1256010
VERSION     BBa_K1256010.1  
FEATURES             Location/Qualifiers
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..289
                     /label=OmpA secretion signal
     CDS             296..2395
                     /label=chitinase
     misc_feature    2396..2401
                     /label=Scar (NsiI + PstI)
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aaaaaacggc
      241 cattgctatc gctgtcgcgt tggcgggctt cgcgactgta gctcaggcgg ctagcatgta
      301 cgacccgatt gtgcgcagtg ccgaacgtgc gacccgaagc tgtcgtcccc gccgcctggc
      361 ctggctgctc gccgtggccg ccggtgccgc tgccctgccc ggcctggcgc aggccgccag
      421 ctgtgccggc gtggcccagt gggaccaggc caagatctac cgggccggcg ataccctgca
      481 gaaaggaggc gtcctctatc gggcgaacca ggacatctgg aacgcgccgc cggaccatcc
      541 ggccggtgcg ccctattaca ccaacctcgg tgcctgcgat ggcagcggcg ccaaccagcc
      601 gccggtggtc agcctgacct cgccggccaa cggcgccacc ttcagcgccg gcagcacggt
      661 caacgtgacc gctaccgcca gtgatccgga cggcagcgtc gccaaggtcg agttcttccg
      721 cgatggcagc acgctgggcg tggccaccag tgcgccgtat gcggccagct ggaccaatgc
      781 cagcgccggc agccacaccc tgcgcgccgt agccaccgac aacaacaacg ccaccagcag
      841 caccgcgacc atcaccatca ccgtcaacgc cgccggtggc gataccaccg caccgagcgt
      901 gccgggcgga ctggcggtcg gcacacgtac cgccaacagc atcgcgctca gctggagccc
      961 gtccaccgac aacaccggtg gcagcggcgt ggccggctat gacgtgtacc gcaatggcag
     1021 cctggtcggt tcaccatcca gtgccagcta cgtcgatggc ggcctgactg catcgaccac
     1081 ctaccgctac cgtgtgcgtg cccgcgacaa cgccggcaac gcgtccgcgc agggcaccga
     1141 gatcagcgcc accacgctgg ccggcgacgg cggcactacc ggcaagcggg tcatcggcta
     1201 cttcacccag tggggcatct acggccgcaa ctaccgggtc aagaacatcg acagcagcgg
     1261 ttcggccgcg cgcctgaccc acatcaacta cgccttcggc aacgtgcgca acaaccgctg
     1321 cgaagtgggc gtcacccagc cgtcggatcc gaacagcggt gccggtggtg atgccttcgc
     1381 cgactacacc aaggccttca gtgccgccga gagtgtcagc ggcagcgccg atacctggga
     1441 ccagccgctg cgcggcaact ggaaccagct caagcagctc aaggccaagt acccgagcat
     1501 gaaggtgctg atctcgctgg gtggctggac ctggtcgcgt ggcttctcca gcgcggcgcg
     1561 cccggaaaac cgccaggcct tcgtcgcctc gtgcatcgat gcctacatca agggcaacct
     1621 gccggtaacc gacggtgccg gtggtgccgg tgccgcgctg ggcgtgttcg acggcatcga
     1681 catcgactgg gagtacccgg tggcgtgcgg catcgaatgc ggcaagccgg aagacaacgc
     1741 caacttcacc gcgctgatgg ccgagttccg ccgccagctc gatgcagtgc gtccgggcct
     1801 gctgctgacg gtggcggtgg gtgccggcat cgacaagatc cgcgtcaccg acccggccgc
     1861 gtaccacccg tacctggact acatcaacgt gatgacctac gacttccacg gcgcgtggga
     1921 tgcgaagacc aatcaccagt cggcgctgtt tgattcgccg aatgatccgt ccaccggcga
     1981 ccagaagctg tacaacagca acgacgccat cgaggccttc atcagccgtg gcgtgcctgc
     2041 cgccaagctc aacctgggca tcggctacta cgggcgtggc tggaccggtg tggccaacgc
     2101 caacaacggc ctgtaccaga ccgcgaccgg cgctgccccg ggcacgtatg aagccggcat
     2161 cgaggactgg aaggtgctga agaacctggc gtggccgggc tacaccgaca acaccgccgg
     2221 tgccacctgg atctacaacg gcagcacgct gtggagcttc gacaccccgg ccaacatcac
     2281 ccgcaagatg ggttacgtga agacccaggg cctgggcggc gcgttcgtct gggagttcag
     2341 tggtgacgat gcgcagggca cgctgaccaa ggcggtcagt gatggcctga agtaaatgca
     2401 g
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