LOCUS BBa_K1256013 4066 bp DNA linear 22-Nov-2024 DEFINITION pSB1C3-Plac-ccdB-Cm-LacI ACCESSION BBa_K1256013 VERSION BBa_K1256013.1 FEATURES Location/Qualifiers promoter 1..200 /label=BBa_R0010 RBS 209..220 /label=BBa_B0034 CDS 227..532 /label=ccdB stem_loop 547..675 /label=BBa_B0015 misc_feature 935..1548 /label=ori stem_loop 1828..1868 /label=BBa_B0012 stem_loop 1877..1956 /label=BBa_B0010 CDS 2029..3108 /label=lacI, BBa_C0012 RBS 3124..3135 /label=BBa_B0034 CDS 3156..3815 /label=Chloramphenicol resistance cassette ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa gctagcatgc agtttaaggt 241 ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat 301 tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa 361 agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac 421 caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca 481 ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat aaggatccta 541 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 601 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 661 ctttctgcgt ttatatacta gtagcggccg ctgcagtccg gcaaaaaagg gcaaggtgtc 721 accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc 781 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 841 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 901 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct 961 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 1021 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 1081 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 1141 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 1201 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 1261 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 1321 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 1381 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 1441 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 1501 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 1561 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 1621 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 1681 tatatatgag taaacttggt ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc 1741 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc 1801 tatcaacagg agtccaagcg atgcagatat aaacgcagaa aggcccaccc gaaggtgagc 1861 cagtgtgact ctagtagaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt 1921 ctttcgactg agcctttcgt tttatttgat gcctggctct agtagagatc tacactagca 1981 ctatcagagt tattaagcta ctaaagcgta gttttcgtcg tttgcagcct gcccgctttc 2041 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 2101 ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg 2161 attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc 2221 cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc 2281 ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat 2341 ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat 2401 gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc 2461 ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg 2521 cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa 2581 tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt 2641 gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc 2701 cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg 2761 cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga 2821 caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga 2881 cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc 2941 cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt 3001 ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata 3061 agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat tcaccatcta 3121 gtatttctcc tctttgaatt gagctcgata tcaaattacg ccccgccctg ccactcatcg 3181 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga 3241 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg 3301 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa 3361 ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag 3421 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa 3481 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg 3541 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat 3601 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc 3661 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag 3721 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata 3781 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat 3841 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa 3901 cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca ttattatcat 3961 gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt 4021 agctttcgct aaggatgatt tctggaattc gcggccgctt ctagag //