LOCUS       BBa_K125800             3450 bp    DNA     linear       25-Nov-2024
DEFINITION  Origin of Replication with Replication Proteins
ACCESSION   BBa_K125800
VERSION     BBa_K125800.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     misc_feature    50..53
                     /label=conserved
     RBS             46..57
                     /label=BBa_B0034
     misc_feature    66..102
                     /label=I14032
     misc_feature    111..125
                     /label=B0030
     CDS             132..1103
                     /label=RepB'
     promoter        1093..1144
                     /label=Promoter[EFRepAC]
     CDS             1164..3291
                     /label=[EFRepAC]
     stem_loop       3418..3450
                     /label=Native Terminator
     CDS             66..3450
                     /label=BBa_K125510
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac
       61 tagagtggtg caaaaccttt cgcggtatgg catgatagcg cctactagag attaaagagg
      121 agaaatacta gatgaagaac gacaggactt tgcaggccat aggccgacag ctcaaggcca
      181 tgggctgtga gcgcttcgat atcggcgtca gggacgcacc caccggccag atgatgaacc
      241 gggaatggtc agccgccgaa gtgctccaga acacgccatg gctcaagcgg atgaatgccc
      301 agggcaatga cgtgtatatc aggcccgccg agcaggagcg gcatggtctg gtgctggtgg
      361 acgacctcag cgagtttgac ctggatgaca tgaaagccga gggccgggag cctgccctgg
      421 tagtggaaac cagcccgaag aactatcagg catgggtcaa ggtggccgac gccgcaggcg
      481 gtgaacttcg ggggcagatt gcccggacgc tggccagcga gtacgacgcc gacccggcca
      541 gcgccgacag ccgccactat ggccgcttgg cgggcttcac caaccgcaag gacaagcaca
      601 ccacccgcgc cggttatcag ccgtgggtgc tgctgcgtga atccaagggc aagaccgcca
      661 ccgctggccc ggcgctggtg cagcaggctg gccagcagat cgagcaggcc cagcggcagc
      721 aggagaaggc ccgcaggctg gccagcctcg aactgcccga gcggcagctt agccgccacc
      781 ggcgcacggc gctggacgag taccgcagcg agatggccgg gctggtcaag cgcttcggtc
      841 atgacctcag caagtgcgac tttatcgccg cgcagaagct ggccagccgg ggccgcagtg
      901 ccgaggaaat cggcaaggcc atggccgagg ccagcccagc gctggcagag cgcaagcccg
      961 gccacgaagc ggattacatc gagcgcaccg tcagcaaggt catgggtctg cccagcgtcc
     1021 agcttgcgcg ggccgagctg gcacgggcac cggcaccccg ccagcgaggc atggacaggg
     1081 gcgggccaga tttcagcatg tagtgcttgc gttggtactc acgcctgtta tactatgagt
     1141 actcacgcac agaagggggt tttatggaat acgaaaaaag cgcttcaggg tcggtctacc
     1201 tgatcaaaag tgacaagggc tattggttgc ccggtggctt tggttatacg tcaaacaagg
     1261 ccgaggctgg ccgcttttca gtcgctgata tggccagcct taaccttgac ggctgcacct
     1321 tgtccttgtt ccgcgaagac aagcctttcg gccccggcaa gtttctcggt gactgatatg
     1381 aaagaccaaa aggacaagca gaccggcgac ctgctggcca gccctgacgc tgtacgccaa
     1441 gcgcgatatg ccgagcgcat gaaggccaaa gggatgcgtc agcgcaagtt ctggctgacc
     1501 gacgacgaat acgaggcgct gcgcgagtgc ctggaagaac tcagagcggc gcagggcggg
     1561 ggtagtgacc ccgccagcgc ctaaccacca actgcctgca aaggaggcaa tcaatggcta
     1621 cccataagcc tatcaatatt ctggaggcgt tcgcagcagc gccgccaccg ctggactacg
     1681 ttttgcccaa catggtggcc ggtacggtcg gggcgctggt gtcgcccggt ggtgccggta
     1741 aatccatgct ggccctgcaa ctggccgcac agattgcagg cgggccggat ctgctggagg
     1801 tgggcgaact gcccaccggc ccggtgatct acctgcccgc cgaagacccg cccaccgcca
     1861 ttcatcaccg cctgcacgcc cttggggcgc acctcagcgc cgaggaacgg caagccgtgg
     1921 ctgacggcct gctgatccag ccgctgatcg gcagcctgcc caacatcatg gccccggagt
     1981 ggttcgacgg cctcaagcgc gccgccgagg gccgccgcct gatggtgctg gacacgctgc
     2041 gccggttcca catcgaggaa gaaaacgcca gcggccccat ggcccaggtc atcggtcgca
     2101 tggaggccat cgccgccgat accgggtgct ctatcgtgtt cctgcaccat gccagcaagg
     2161 gcgcggccat gatgggcgca ggcgaccagc agcaggccag ccggggcagc tcggtactgg
     2221 tcgataacat ccgctggcag tcctacctgt cgagcatgac cagcgccgag gccgaggaat
     2281 ggggtgtgga cgacgaccag cgccggttct tcgtccgctt cggtgtgagc aaggccaact
     2341 atggcgcacc gttcgctgat cggtggttca ggcggcatga cggcggggtg ctcaagcccg
     2401 ccgtgctgga gaggcagcgc aagagcaagg gggtgccccg tggtgaagcc taagaacaag
     2461 cacagcctca gccacgtccg gcacgacccg gcgcactgtc tggcccccgg cctgttccgt
     2521 gccctcaagc ggggcgagcg caagcgcagc aagctggacg tgacgtatga ctacggcgac
     2581 ggcaagcgga tcgagttcag cggcccggag ccgctgggcg ctgatgatct gcgcatcctg
     2641 caagggctgg tggccatggc tgggcctaat ggcctagtgc ttggcccgga acccaagacc
     2701 gaaggcggac ggcagctccg gctgttcctg gaacccaagt gggaggccgt caccgctgaa
     2761 tgccatgtgg tcaaaggtag ctatcgggcg ctggcaaagg aaatcggggc agaggtcgat
     2821 agtggtgggg cgctcaagca catacaggac tgcatcgagc gcctttggaa ggtatccatc
     2881 atcgcccaga atggccgcaa gcggcagggg tttcggctgc tgtcggagta cgccagcgac
     2941 gaggcggacg ggcgcctgta cgtggccctg aaccccttga tcgcgcaggc cgtcatgggt
     3001 ggcggccagc atgtgcgcat cagcatggac gaggtgcggg cgctggacag cgaaaccgcc
     3061 cgcctgctgc accagcggct gtgtggctgg atcgaccccg gcaaaaccgg caaggcttcc
     3121 atagatacct tgtgcggcta tgtctggccg tcagaggcca gtggttcgac catgcgcaag
     3181 cgccgcaagc gggtgcgcga ggcgttgccg gagctggtcg cgctgggctg gacggtaacc
     3241 gagttcgcgg cgggcaagta cgacatcacc cggcccaagg cggcaggctg acccccccca
     3301 ctctattgta aacaagacat ttttatcttt tatattcaat ggcttatttt cctgctaatt
     3361 ggtaatacca tgaaaaatac catgctcaga aaaggcttaa caatattttg aaaaattgcc
     3421 tactgagcgc tgccgcacag ctccataggc
//