LOCUS       BBa_K125804             1014 bp    DNA     linear       23-Dec-2024
DEFINITION  RBS-phaE
ACCESSION   BBa_K125804
VERSION     BBa_K125804.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..21
                     /label=start
     misc_feature    1009..1011
                     /label=original stop codon
     misc_feature    1012..1014
                     /label=inserted second stop
     misc_feature    873..873
                     /label=E. coli codon optimized, Ile ATT -> ATC, avoided 
                     rare Ile ATA
     CDS             19..1014
                     /label=BBa_K125504
ORIGIN
        1 aaagaggaga aatactagat ggaatcgaca aataaaacct ggacagaact catgactccc
       61 ctcagtcagt tttggctgga gtccagtagc caggcttgga agaattggtt tgacctcatg
      121 gccaagggcg gggccggcgc catgatgggc tcggctcccc agtcctttga gtctttgccc
      181 caacagttcc tccaatccca gcagttttat ggagagttac ttaagctttc ttttgaagct
      241 tggcaaagcc tgtggcctaa attggataat ggttcggcgc caggggcagt gcagggctac
      301 ctaaaacagc tacaaaccca aattgagcaa tataccgcca ccacccaagc tctccaaggg
      361 gacatggatg gtttatggca gtgttacatc aaggaagtac aaagattttc ccaactctgg
      421 ctctccacct ggcagagtag cgtcgccccc ctgggcaaat tacccaccgg ggacatccat
      481 gcttggttag atttaaataa tctctacggc gatgccctct acaacaaaaa cctgagcagt
      541 tttatgcgat cgcctttgct ggggcccagt cgggaaatga atggcaaatt attgcgggcc
      601 tttgacgatt gggttaagtt atcccaggcc atggcagact atcaattact ggaagcagat
      661 attcaatacc ggggctttgc tgctttgatg gaagatttac tggcccgggc taaggaagat
      721 aaacccgtta aaacctggaa ggaatttcaa caacggtggg cgatcgccgc tgaccaagtg
      781 tttgaagaag ctttttgtga ggaaaaaaat ctgaaagtac ggggcaaatt catcaatgcc
      841 ttgaatcgtt atcgcattca gcaacaggag atcctagaag catggttaaa aatgctgaac
      901 ctccctaccc gctcagaggt ggatgaaatt catcaaacca tttatcagtt gcggaaagaa
      961 gttaaaagtt tgaaaaaacg attgggagaa acagaagcaa acccaggcta ataa
//