BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K125504
1
BBa_K125504
phaE BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1829)
2008-08-09T11:00:00Z
2015-05-08T01:09:45Z
Synechocystis sp. PCC6803
phaE
false
false
_179_
0
2680
9
It's complicated
false
PHB biosynthesis pathway
false
Norman Wang
annotation1971714
1
start
range1971714
1
1
3
annotation1971715
1
original stop codon
range1971715
1
991
993
annotation1971717
1
E. coli codon optimized, Ile ATT -> ATC, avoided rare Ile ATA
range1971717
1
855
855
annotation1971716
1
inserted second stop
range1971716
1
994
996
BBa_K125804
1
BBa_K125804
RBS-phaE
2008-09-02T11:00:00Z
2015-05-08T01:09:45Z
composite part
RBS 1.0 + phaE gene
false
false
_179_
0
2680
9
It's complicated
true
composite part
false
Norman Wang
component1974245
1
BBa_K125504
component1974240
1
BBa_B0034
annotation1974240
1
BBa_B0034
range1974240
1
1
12
annotation1974245
1
BBa_K125504
range1974245
1
19
1014
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K125504_sequence
1
atggaatcgacaaataaaacctggacagaactcatgactcccctcagtcagttttggctggagtccagtagccaggcttggaagaattggtttgacctcatggccaagggcggggccggcgccatgatgggctcggctccccagtcctttgagtctttgccccaacagttcctccaatcccagcagttttatggagagttacttaagctttcttttgaagcttggcaaagcctgtggcctaaattggataatggttcggcgccaggggcagtgcagggctacctaaaacagctacaaacccaaattgagcaatataccgccaccacccaagctctccaaggggacatggatggtttatggcagtgttacatcaaggaagtacaaagattttcccaactctggctctccacctggcagagtagcgtcgcccccctgggcaaattacccaccggggacatccatgcttggttagatttaaataatctctacggcgatgccctctacaacaaaaacctgagcagttttatgcgatcgcctttgctggggcccagtcgggaaatgaatggcaaattattgcgggcctttgacgattgggttaagttatcccaggccatggcagactatcaattactggaagcagatattcaataccggggctttgctgctttgatggaagatttactggcccgggctaaggaagataaacccgttaaaacctggaaggaatttcaacaacggtgggcgatcgccgctgaccaagtgtttgaagaagctttttgtgaggaaaaaaatctgaaagtacggggcaaattcatcaatgccttgaatcgttatcgcattcagcaacaggagatcctagaagcatggttaaaaatgctgaacctccctacccgctcagaggtggatgaaattcatcaaaccatttatcagttgcggaaagaagttaaaagtttgaaaaaacgattgggagaaacagaagcaaacccaggctaataa
BBa_K125804_sequence
1
aaagaggagaaatactagatggaatcgacaaataaaacctggacagaactcatgactcccctcagtcagttttggctggagtccagtagccaggcttggaagaattggtttgacctcatggccaagggcggggccggcgccatgatgggctcggctccccagtcctttgagtctttgccccaacagttcctccaatcccagcagttttatggagagttacttaagctttcttttgaagcttggcaaagcctgtggcctaaattggataatggttcggcgccaggggcagtgcagggctacctaaaacagctacaaacccaaattgagcaatataccgccaccacccaagctctccaaggggacatggatggtttatggcagtgttacatcaaggaagtacaaagattttcccaactctggctctccacctggcagagtagcgtcgcccccctgggcaaattacccaccggggacatccatgcttggttagatttaaataatctctacggcgatgccctctacaacaaaaacctgagcagttttatgcgatcgcctttgctggggcccagtcgggaaatgaatggcaaattattgcgggcctttgacgattgggttaagttatcccaggccatggcagactatcaattactggaagcagatattcaataccggggctttgctgctttgatggaagatttactggcccgggctaaggaagataaacccgttaaaacctggaaggaatttcaacaacggtgggcgatcgccgctgaccaagtgtttgaagaagctttttgtgaggaaaaaaatctgaaagtacggggcaaattcatcaatgccttgaatcgttatcgcattcagcaacaggagatcctagaagcatggttaaaaatgctgaacctccctacccgctcagaggtggatgaaattcatcaaaccatttatcagttgcggaaagaagttaaaagtttgaaaaaacgattgggagaaacagaagcaaacccaggctaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z