BBa_K125500 1 BBa_K125500 GFP fusion brick 2008-07-24T11:00:00Z 2016-01-25T02:34:56Z The GFP fusion brick was derived from the GFP BioBrick, [http://partsregistry.org/Part:BBa_E0040 BBa_E0040]. false false _179_ 4206 3229 9 In stock false The first two nucleotides of the GFP start codon were removed to create this fusion brick. false Grace Kwan annotation1969908 1 deletion of TG range1969908 1 1 1 annotation1968260 1 GFP fusion brick range1968260 1 1 718 BBa_K125820 1 BBa_K125820 lac + rbs + slr2016 + GFP fusionbrick 2008-10-24T11:00:00Z 2015-05-08T01:09:45Z The lac promoter and rbs were current BioBrick parts BBa_I14032 and BBa_B0034, respectively. The signal sequence slr2016 is derived from the genome of Synechocystis sp. PCC6803. The GFP fusion is derived from the BioBrick part for GFP, BBa_E0040. This device codes for a signal sequence-GFP fusion protein designed for secretion of GFP by Synechocystis sp. PCC6803. false false _179_ 0 3177 9 It's complicated false false Krystle Salazar and Grace Kwan component1987871 1 BBa_K125310 component1987873 1 BBa_K125500 component1987867 1 BBa_I14032 component1987869 1 BBa_B0034 annotation1987871 1 BBa_K125310 range1987871 1 66 118 annotation1987869 1 BBa_B0034 range1987869 1 46 57 annotation1987867 1 BBa_I14032 range1987867 1 1 37 annotation1987873 1 BBa_K125500 range1987873 1 127 844 BBa_K125310 1 slr2016 SS slr2016 signal sequence from cyanobacterium Synechocystis; secretes protein 2008-07-24T11:00:00Z 2015-05-08T01:09:44Z The ''slr2016'' signal sequence is located at the amino terminus of slr2016 polypeptides synthesized by ''Synechocystis'' sp. PCC 6803. false false _179_ 0 3229 9 In stock false false Krystle Salazar and Grace Kwan annotation1968259 1 slr2016 signal sequence range1968259 1 1 53 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I14032 1 P(Lac) IQ promoter P(Lac) IQ 2004-08-03T11:00:00Z 2015-08-31T04:07:37Z Plasmid pMAL-p2X Released HQ 2013 Constitutive Promoter, High Transcription false true _4_ 0 171 7 In stock false true Vikram Vijayan, Allen Hsu, Lawrence Fomundam annotation1028342 1 P(Lac) IQ range1028342 1 1 37 annotation1028343 1 -35 range1028343 1 3 8 annotation1028344 1 -10 range1028344 1 26 31 BBa_K125500_sequence 1 cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataat BBa_B0034_sequence 1 aaagaggagaaa BBa_K125310_sequence 1 tggcagcaaaacaactatggaaaattttcaatcctagaccgatgaagggtgga BBa_I14032_sequence 1 tggtgcaaaacctttcgcggtatggcatgatagcgcc BBa_K125820_sequence 1 tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagagtggcagcaaaacaactatggaaaattttcaatcctagaccgatgaagggtggatactagagcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z