LOCUS       BBa_K1260004            1088 bp    DNA     linear       22-Dec-2024
DEFINITION  0030+ScFv+double terminator
ACCESSION   BBa_K1260004
VERSION     BBa_K1260004.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     misc_feature    22..24
                     /label=start
     misc_feature    22..90
                     /label=ssDsbA
     CDS             109..840
                     /label=AFB1-ScFv
     misc_feature    91..108
                     /label=linker 1
     misc_feature    841..858
                     /label=6*his-tag
     misc_feature    868..912
                     /label=linker 2
     misc_feature    912..945
                     /label=SH3 Ligand
     misc_feature    946..951
                     /label=stop
     CDS             22..951
                     /label=BBa_K1260000
     misc_feature    1..951
                     /label=BBa_K1260003
     stem_loop       971..1014
                     /label=stem_loop
     misc_feature    960..1039
                     /label=BBa_B0010
     terminator      960..1039
                     /label=BBa_B0010
     stem_loop       1055..1074
                     /label=T7 TE
     misc_feature    1081..1081
                     /label=stop
     polyA_site      1075..1088
                     /label=polya
     misc_feature    1048..1088
                     /label=BBa_B0012
     terminator      1048..1088
                     /label=BBa_B0012
     terminator      960..1088
                     /label=BBa_B0015
ORIGIN
        1 attaaagagg agaaatacta gatgaaaaag acagctatcg cgattgcagt ggcactggct
       61 ggtttcgcta ccgtagcgca ggccactaga ggtagcggca gcggtagcac tagagcacag
      121 gtgaaactgc aacagagcgg aaccgaagtt gttaaaccgg gggcaagcgt taagctgagc
      181 tgtaaggcaa gcggttatat ttttaccagc tatgatattg attgggttcg tcagaccccg
      241 gaacagggtc tggaatggat tggttggatt tttccgggtg aaggtagcac cgaatataat
      301 gaaaaattca aaggtcgtgc aaccctgagc gtagataaaa gcagcagcac cgcatatatg
      361 gaactgacca ggctgaccag cgaagatagc gcagtttatt tttgtgcacg tggtgattat
      421 tatcgtcgtt attttgacct gtggggtcag ggtaccaccg ttaccgtttc ctcaggtgga
      481 ggcggttcag gcggaggtgg ctctggcggt ggcggatcgg atattgaact gacccagagc
      541 ccggcaatta tgagcgcaag cccgggtgaa cgtgtcacca tgacctgtag cgcaagcagc
      601 agcattcgtt atatttattg gtatcagcag aaaccgggta gcagcccgcg tctgctgatt
      661 tatgatacaa gcaatgttgc accgggtgtt ccttttcgtt ttagcggtag cggtagcggt
      721 accagctata gcctgaccat taatcgtatg gaagcagaag atgcagcaac ctattattgt
      781 caggaatgga gcggttatcc gtacaccttt ggtggtggta ccaaactgga actgaaacgt
      841 catcatcatc atcatcacac tagagctgag gccgccgcaa aagaagcagc agctaaggaa
      901 gctgcggcga agccccctcc agcgctgccg ccaaaacgtc gccggtaata atactagagc
      961 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1021 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1081 cgtttata
//