BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_K1260000
1
BBa_K1260000
AFB1 ScFv with a his-tag, a SH3 ligand and a signal peptide
2014-04-24T11:00:00Z
2015-05-08T01:09:45Z
The part is constructed artificially.
AFB1 ScFv with a his-tag, a SH3 ligand and a signal peptide. The signal peptide is at the beginning of the sequence. With the part, you can let your E.coli produce antibody to target the aflatoxin B1.
false
false
_1618_
0
19163
9
It's complicated
true
We have improved the codons of the coding sequence.
false
Zhengfeng Liu
annotation2372757
1
6*his-tag
range2372757
1
820
837
annotation2372760
1
stop
range2372760
1
925
930
annotation2372758
1
linker 2
range2372758
1
847
891
annotation2372754
1
ssDsbA
range2372754
1
1
69
annotation2372756
1
linker 1
range2372756
1
70
87
annotation2372755
1
AFB1-ScFv
range2372755
1
88
819
annotation2372753
1
start
range2372753
1
1
3
annotation2372759
1
SH3 Ligand
range2372759
1
891
924
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_K1260003
1
BBa_K1260003
RBS B0030+ ScFv
2014-05-23T11:00:00Z
2015-05-08T01:09:45Z
It comes from BBa_K1260000 and BBa_B0030.
This is a composite part of BBa_K1260000 and BBa_B0030.
false
false
_1618_
0
19163
9
It's complicated
false
It has a rbs and a cds of ScFv.
false
Zhengfeng Liu
component2378252
1
BBa_B0030
component2378262
1
BBa_K1260000
annotation2378262
1
BBa_K1260000
range2378262
1
22
951
annotation2378252
1
BBa_B0030
range2378252
1
1
15
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_K1260004
1
BBa_K1260004
0030+ScFv+double terminator
2014-05-23T11:00:00Z
2015-05-08T01:09:45Z
It comes from BBa_K1260003 and BBa_B0015.
It comes from BBa_K1260003 and BBa_B0015. It has a RBS, a ScFv and a double termintor in total.
false
false
_1618_
0
19163
9
It's complicated
false
The end codon and the terminator has been added.
false
Zhengfeng Liu
component2378282
1
BBa_B0015
component2378275
1
BBa_K1260003
annotation2378275
1
BBa_K1260003
range2378275
1
1
951
annotation2378282
1
BBa_B0015
range2378282
1
960
1088
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1260004_sequence
1
attaaagaggagaaatactagatgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggccactagaggtagcggcagcggtagcactagagcacaggtgaaactgcaacagagcggaaccgaagttgttaaaccgggggcaagcgttaagctgagctgtaaggcaagcggttatatttttaccagctatgatattgattgggttcgtcagaccccggaacagggtctggaatggattggttggatttttccgggtgaaggtagcaccgaatataatgaaaaattcaaaggtcgtgcaaccctgagcgtagataaaagcagcagcaccgcatatatggaactgaccaggctgaccagcgaagatagcgcagtttatttttgtgcacgtggtgattattatcgtcgttattttgacctgtggggtcagggtaccaccgttaccgtttcctcaggtggaggcggttcaggcggaggtggctctggcggtggcggatcggatattgaactgacccagagcccggcaattatgagcgcaagcccgggtgaacgtgtcaccatgacctgtagcgcaagcagcagcattcgttatatttattggtatcagcagaaaccgggtagcagcccgcgtctgctgatttatgatacaagcaatgttgcaccgggtgttccttttcgttttagcggtagcggtagcggtaccagctatagcctgaccattaatcgtatggaagcagaagatgcagcaacctattattgtcaggaatggagcggttatccgtacacctttggtggtggtaccaaactggaactgaaacgtcatcatcatcatcatcacactagagctgaggccgccgcaaaagaagcagcagctaaggaagctgcggcgaagccccctccagcgctgccgccaaaacgtcgccggtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1260000_sequence
1
atgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggccactagaggtagcggcagcggtagcactagagcacaggtgaaactgcaacagagcggaaccgaagttgttaaaccgggggcaagcgttaagctgagctgtaaggcaagcggttatatttttaccagctatgatattgattgggttcgtcagaccccggaacagggtctggaatggattggttggatttttccgggtgaaggtagcaccgaatataatgaaaaattcaaaggtcgtgcaaccctgagcgtagataaaagcagcagcaccgcatatatggaactgaccaggctgaccagcgaagatagcgcagtttatttttgtgcacgtggtgattattatcgtcgttattttgacctgtggggtcagggtaccaccgttaccgtttcctcaggtggaggcggttcaggcggaggtggctctggcggtggcggatcggatattgaactgacccagagcccggcaattatgagcgcaagcccgggtgaacgtgtcaccatgacctgtagcgcaagcagcagcattcgttatatttattggtatcagcagaaaccgggtagcagcccgcgtctgctgatttatgatacaagcaatgttgcaccgggtgttccttttcgttttagcggtagcggtagcggtaccagctatagcctgaccattaatcgtatggaagcagaagatgcagcaacctattattgtcaggaatggagcggttatccgtacacctttggtggtggtaccaaactggaactgaaacgtcatcatcatcatcatcacactagagctgaggccgccgcaaaagaagcagcagctaaggaagctgcggcgaagccccctccagcgctgccgccaaaacgtcgccggtaataa
BBa_K1260003_sequence
1
attaaagaggagaaatactagatgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggccactagaggtagcggcagcggtagcactagagcacaggtgaaactgcaacagagcggaaccgaagttgttaaaccgggggcaagcgttaagctgagctgtaaggcaagcggttatatttttaccagctatgatattgattgggttcgtcagaccccggaacagggtctggaatggattggttggatttttccgggtgaaggtagcaccgaatataatgaaaaattcaaaggtcgtgcaaccctgagcgtagataaaagcagcagcaccgcatatatggaactgaccaggctgaccagcgaagatagcgcagtttatttttgtgcacgtggtgattattatcgtcgttattttgacctgtggggtcagggtaccaccgttaccgtttcctcaggtggaggcggttcaggcggaggtggctctggcggtggcggatcggatattgaactgacccagagcccggcaattatgagcgcaagcccgggtgaacgtgtcaccatgacctgtagcgcaagcagcagcattcgttatatttattggtatcagcagaaaccgggtagcagcccgcgtctgctgatttatgatacaagcaatgttgcaccgggtgttccttttcgttttagcggtagcggtagcggtaccagctatagcctgaccattaatcgtatggaagcagaagatgcagcaacctattattgtcaggaatggagcggttatccgtacacctttggtggtggtaccaaactggaactgaaacgtcatcatcatcatcatcacactagagctgaggccgccgcaaaagaagcagcagctaaggaagctgcggcgaagccccctccagcgctgccgccaaaacgtcgccggtaataa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z