BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_K1260001
1
BBa_K1260001
AFB1 ScFv with a his-tag and a SH3 ligand
2014-04-24T11:00:00Z
2015-05-08T01:09:45Z
The part is constructed artificially.
AFB1 ScFv with a his-tag and a SH3 ligand. With the part, you can let your E.coli produce antibody to target the aflatoxin B1.( The part doesn't have a signal peptide with it.)
false
false
_1618_
0
19163
9
It's complicated
false
We have improved the codons of the coding sequence.
false
Zhengfeng Liu
annotation2372763
1
AFB1-ScFv
range2372763
1
4
735
annotation2372761
1
start
range2372761
1
1
3
annotation2372764
1
linker
range2372764
1
763
807
annotation2372766
1
stop
range2372766
1
841
846
annotation2372762
1
6*his-tag
range2372762
1
736
753
annotation2372765
1
SH3 Ligand
range2372765
1
808
840
BBa_K1260006
1
BBa_K1260006
0030+ScFv（nonsp）
2014-05-23T11:00:00Z
2015-05-08T01:09:45Z
It comes from BBa_B0030 and BBa_K1260001
It is made up of BBa_B0030 and BBa_K1260001. We add a RBS inn front of the coding sequence.
false
false
_1618_
0
19163
9
It's complicated
false
The signal peptide coding sequence has been removed.
false
Zhengfeng Liu
component2378307
1
BBa_B0030
component2378315
1
BBa_K1260001
annotation2378307
1
BBa_B0030
range2378307
1
1
15
annotation2378315
1
BBa_K1260001
range2378315
1
22
867
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K1260006_sequence
1
attaaagaggagaaatactagatgactagagcacaggtgaaactgcaacagagcggaaccgaagttgttaaaccgggggcaagcgttaagctgagctgtaaggcaagcggttatatttttaccagctatgatattgattgggttcgtcagaccccggaacagggtctggaatggattggttggatttttccgggtgaaggtagcaccgaatataatgaaaaattcaaaggtcgtgcaaccctgagcgtagataaaagcagcagcaccgcatatatggaactgaccaggctgaccagcgaagatagcgcagtttatttttgtgcacgtggtgattattatcgtcgttattttgacctgtggggtcagggtaccaccgttaccgtttcctcaggtggaggcggttcaggcggaggtggctctggcggtggcggatcggatattgaactgacccagagcccggcaattatgagcgcaagcccgggtgaacgtgtcaccatgacctgtagcgcaagcagcagcattcgttatatttattggtatcagcagaaaccgggtagcagcccgcgtctgctgatttatgatacaagcaatgttgcaccgggtgttccttttcgttttagcggtagcggtagcggtaccagctatagcctgaccattaatcgtatggaagcagaagatgcagcaacctattattgtcaggaatggagcggttatccgtacacctttggtggtggtaccaaactggaactgaaacgtcatcatcatcatcatcacactagagctgaggccgccgcaaaagaagcagcagctaaggaagctgcggcgaagccccctccagcgctgccgccaaaacgtcgccggtaataa
BBa_K1260001_sequence
1
atgactagagcacaggtgaaactgcaacagagcggaaccgaagttgttaaaccgggggcaagcgttaagctgagctgtaaggcaagcggttatatttttaccagctatgatattgattgggttcgtcagaccccggaacagggtctggaatggattggttggatttttccgggtgaaggtagcaccgaatataatgaaaaattcaaaggtcgtgcaaccctgagcgtagataaaagcagcagcaccgcatatatggaactgaccaggctgaccagcgaagatagcgcagtttatttttgtgcacgtggtgattattatcgtcgttattttgacctgtggggtcagggtaccaccgttaccgtttcctcaggtggaggcggttcaggcggaggtggctctggcggtggcggatcggatattgaactgacccagagcccggcaattatgagcgcaagcccgggtgaacgtgtcaccatgacctgtagcgcaagcagcagcattcgttatatttattggtatcagcagaaaccgggtagcagcccgcgtctgctgatttatgatacaagcaatgttgcaccgggtgttccttttcgttttagcggtagcggtagcggtaccagctatagcctgaccattaatcgtatggaagcagaagatgcagcaacctattattgtcaggaatggagcggttatccgtacacctttggtggtggtaccaaactggaactgaaacgtcatcatcatcatcatcacactagagctgaggccgccgcaaaagaagcagcagctaaggaagctgcggcgaagccccctccagcgctgccgccaaaacgtcgccggtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z