LOCUS       BBa_K1260007            1004 bp    DNA     linear       23-Jun-2024
DEFINITION  RBS B0030+ ScFv（nonsp）+double terminator
ACCESSION   BBa_K1260007
VERSION     BBa_K1260007.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     misc_feature    22..24
                     /label=start
     misc_feature    757..774
                     /label=6*his-tag
     CDS             25..756
                     /label=AFB1-ScFv
     misc_feature    784..828
                     /label=linker
     misc_feature    829..861
                     /label=SH3 Ligand
     misc_feature    862..867
                     /label=stop
     CDS             22..867
                     /label=BBa_K1260001
     stem_loop       887..930
                     /label=stem_loop
     misc_feature    876..955
                     /label=BBa_B0010
     terminator      876..955
                     /label=BBa_B0010
     stem_loop       971..990
                     /label=T7 TE
     misc_feature    997..997
                     /label=stop
     polyA_site      991..1004
                     /label=polya
     misc_feature    964..1004
                     /label=BBa_B0012
     terminator      964..1004
                     /label=BBa_B0012
     terminator      876..1004
                     /label=BBa_B0015
ORIGIN
        1 attaaagagg agaaatacta gatgactaga gcacaggtga aactgcaaca gagcggaacc
       61 gaagttgtta aaccgggggc aagcgttaag ctgagctgta aggcaagcgg ttatattttt
      121 accagctatg atattgattg ggttcgtcag accccggaac agggtctgga atggattggt
      181 tggatttttc cgggtgaagg tagcaccgaa tataatgaaa aattcaaagg tcgtgcaacc
      241 ctgagcgtag ataaaagcag cagcaccgca tatatggaac tgaccaggct gaccagcgaa
      301 gatagcgcag tttatttttg tgcacgtggt gattattatc gtcgttattt tgacctgtgg
      361 ggtcagggta ccaccgttac cgtttcctca ggtggaggcg gttcaggcgg aggtggctct
      421 ggcggtggcg gatcggatat tgaactgacc cagagcccgg caattatgag cgcaagcccg
      481 ggtgaacgtg tcaccatgac ctgtagcgca agcagcagca ttcgttatat ttattggtat
      541 cagcagaaac cgggtagcag cccgcgtctg ctgatttatg atacaagcaa tgttgcaccg
      601 ggtgttcctt ttcgttttag cggtagcggt agcggtacca gctatagcct gaccattaat
      661 cgtatggaag cagaagatgc agcaacctat tattgtcagg aatggagcgg ttatccgtac
      721 acctttggtg gtggtaccaa actggaactg aaacgtcatc atcatcatca tcacactaga
      781 gctgaggccg ccgcaaaaga agcagcagct aaggaagctg cggcgaagcc ccctccagcg
      841 ctgccgccaa aacgtcgccg gtaataatac tagagccagg catcaaataa aacgaaaggc
      901 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
      961 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//