LOCUS       BBa_K1260008            1142 bp    DNA     linear       15-Nov-2024
DEFINITION  pbad- ScFv(nonsp) (whole device)
ACCESSION   BBa_K1260008
VERSION     BBa_K1260008.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206001
     RBS             139..153
                     /label=RBS-1\Strong
     RBS             146..150
                     /label=RBS
     misc_feature    139..153
                     /label=BBa_B0030
     RBS             139..153
                     /label=BBa_B0030
     misc_feature    160..162
                     /label=start
     misc_feature    895..912
                     /label=6*his-tag
     CDS             163..894
                     /label=AFB1-ScFv
     misc_feature    922..966
                     /label=linker
     misc_feature    967..999
                     /label=SH3 Ligand
     misc_feature    1000..1005
                     /label=stop
     CDS             160..1005
                     /label=BBa_K1260001
     stem_loop       1025..1068
                     /label=stem_loop
     misc_feature    1014..1093
                     /label=BBa_B0010
     terminator      1014..1093
                     /label=BBa_B0010
     stem_loop       1109..1128
                     /label=T7 TE
     misc_feature    1135..1135
                     /label=stop
     polyA_site      1129..1142
                     /label=polya
     misc_feature    1102..1142
                     /label=BBa_B0012
     terminator      1102..1142
                     /label=BBa_B0012
     terminator      1014..1142
                     /label=BBa_B0015
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agctttttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagat taaagaggag aaatactaga tgactagagc acaggtgaaa
      181 ctgcaacaga gcggaaccga agttgttaaa ccgggggcaa gcgttaagct gagctgtaag
      241 gcaagcggtt atatttttac cagctatgat attgattggg ttcgtcagac cccggaacag
      301 ggtctggaat ggattggttg gatttttccg ggtgaaggta gcaccgaata taatgaaaaa
      361 ttcaaaggtc gtgcaaccct gagcgtagat aaaagcagca gcaccgcata tatggaactg
      421 accaggctga ccagcgaaga tagcgcagtt tatttttgtg cacgtggtga ttattatcgt
      481 cgttattttg acctgtgggg tcagggtacc accgttaccg tttcctcagg tggaggcggt
      541 tcaggcggag gtggctctgg cggtggcgga tcggatattg aactgaccca gagcccggca
      601 attatgagcg caagcccggg tgaacgtgtc accatgacct gtagcgcaag cagcagcatt
      661 cgttatattt attggtatca gcagaaaccg ggtagcagcc cgcgtctgct gatttatgat
      721 acaagcaatg ttgcaccggg tgttcctttt cgttttagcg gtagcggtag cggtaccagc
      781 tatagcctga ccattaatcg tatggaagca gaagatgcag caacctatta ttgtcaggaa
      841 tggagcggtt atccgtacac ctttggtggt ggtaccaaac tggaactgaa acgtcatcat
      901 catcatcatc acactagagc tgaggccgcc gcaaaagaag cagcagctaa ggaagctgcg
      961 gcgaagcccc ctccagcgct gccgccaaaa cgtcgccggt aataatacta gagccaggca
     1021 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1081 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     1141 ta
//