BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_K1260009
1
BBa_K1260009
RBS 0030+ membrane protein LGT
2014-06-07T11:00:00Z
2015-05-08T01:09:45Z
It comes from BBa_B0030 and BBa_K1260002.
It is a composite part constructed by BBa_K1260002 and BBa_B0030
false
false
_1618_
0
19163
9
It's complicated
false
The RBS is in front of the BBa_K1260002
false
Zhengfeng Liu
component2378689
1
BBa_K1260002
component2378674
1
BBa_B0030
component2378688
1
BBa_K1260002
component2378675
1
BBa_B0030
annotation2378675
1
BBa_B0030
range2378675
1
1
15
annotation2378689
1
BBa_K1260002
range2378689
1
22
1233
annotation2378688
1
BBa_K1260002
range2378688
1
22
1233
annotation2378674
1
BBa_B0030
range2378674
1
1
15
BBa_K1260002
1
BBa_K1260002
membrane protein LGT with a SH3 domain
2014-05-22T11:00:00Z
2015-05-08T01:09:45Z
It is reconstructed from the BBa_K771007.
The gene has a membrane protein LGT and a SH3 domain. With the fusion protein it expressd on the E.coli's membrane, you can attach the proteins with a SH3 ligand on its tail.
false
false
_1618_
0
19163
9
It's complicated
false
The part is a little bit difficult to be PCR because of the same sequence of two linkers on it.
false
Zhengfeng Liu
annotation2378213
1
membrane protein LGT
range2378213
1
328
1209
annotation2378211
1
SH3 domain
range2378211
1
1
282
annotation2378210
1
start codon
range2378210
1
1
3
annotation2378212
1
linker
range2378212
1
282
327
annotation2378214
1
stop codon
range2378214
1
1210
1212
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K1260002_sequence
1
atgaaaaagatttggctggcgctggctggtttagttttagcgtttagcgcaagcgcagctgaggccgccgcaaaagaagcagcagctaaggaagctgcggcgaaggcagagtatgtgcgggccctgtttgactttaatgggaatgatgaagaagaccttccctttaagaaaggagacatcctgagaatccgggataagcctgaagagcagtggtggaatgcagaggacagcgaaggaaagagggggatgattcctgtcccttacgtggagaagtatactagagaggccgccgcaaaagaagcagcagctaaggaagctgcggcgaagatgaccagtagctatctgcattttccggagtttgatccggtcattttctcaataggacccgtggcgcttcactggtacggcctgatgtatctggtgggtttcatttttgcaatgtggctggcaacacgacgggcgaatcgtccgggcagcggctggaccaaaaatgaagttgaaaacttactctatgcgggcttcctcggcgtcttcctcgggggacgtattggttatgttctgttctacaatttcccgcagtttatggccgatccgctgtatctgttccgtgtctgggacggcggcatgtctttccacggcggcctgattggcgttatcgtggtgatgattatcttcgcccgccgtactaaacgttccttcttccaggtctctgattttatcgcaccactcattccgtttggtcttggtgccgggcgtctgggcaactttattaacggtgaattgtggggccgcgttgacccgaacttcccgtttgccatgctgttccctggctcccgtacagaagatattttgctgctgcaaaccaacccgcagtggcaatccattttcgacacttacggtgtgctgccgcgccacccatcacagctttacgagctgctgctggaaggtgtggtgctgtttattatcctcaacctgtatattcgtaaaccacgcccaatgggagctgtctcaggtttgttcctgattggttacggcgcgtttcgcatcattgttgagtttttccgccagcccgacgcgcagtttaccggtgcctgggtgcagtacatcagcatggggcaaattctttccatcccgatgattgtcgcgggtgtgatcatgatggtctgggcatatcgtcgcagcccacagcaacaggtttccactagagcttaa
BBa_K1260009_sequence
1
attaaagaggagaaatactagatgaaaaagatttggctggcgctggctggtttagttttagcgtttagcgcaagcgcagctgaggccgccgcaaaagaagcagcagctaaggaagctgcggcgaaggcagagtatgtgcgggccctgtttgactttaatgggaatgatgaagaagaccttccctttaagaaaggagacatcctgagaatccgggataagcctgaagagcagtggtggaatgcagaggacagcgaaggaaagagggggatgattcctgtcccttacgtggagaagtatactagagaggccgccgcaaaagaagcagcagctaaggaagctgcggcgaagatgaccagtagctatctgcattttccggagtttgatccggtcattttctcaataggacccgtggcgcttcactggtacggcctgatgtatctggtgggtttcatttttgcaatgtggctggcaacacgacgggcgaatcgtccgggcagcggctggaccaaaaatgaagttgaaaacttactctatgcgggcttcctcggcgtcttcctcgggggacgtattggttatgttctgttctacaatttcccgcagtttatggccgatccgctgtatctgttccgtgtctgggacggcggcatgtctttccacggcggcctgattggcgttatcgtggtgatgattatcttcgcccgccgtactaaacgttccttcttccaggtctctgattttatcgcaccactcattccgtttggtcttggtgccgggcgtctgggcaactttattaacggtgaattgtggggccgcgttgacccgaacttcccgtttgccatgctgttccctggctcccgtacagaagatattttgctgctgcaaaccaacccgcagtggcaatccattttcgacacttacggtgtgctgccgcgccacccatcacagctttacgagctgctgctggaaggtgtggtgctgtttattatcctcaacctgtatattcgtaaaccacgcccaatgggagctgtctcaggtttgttcctgattggttacggcgcgtttcgcatcattgttgagtttttccgccagcccgacgcgcagtttaccggtgcctgggtgcagtacatcagcatggggcaaattctttccatcccgatgattgtcgcgggtgtgatcatgatggtctgggcatatcgtcgcagcccacagcaacaggtttccactagagcttaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z