LOCUS       BBa_K1260010            1370 bp    DNA     linear       30-Jun-2024
DEFINITION  RBS 0030+ membrane protein LGT+double terminter
ACCESSION   BBa_K1260010
VERSION     BBa_K1260010.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     misc_feature    22..24
                     /label=start codon
     CDS             22..303
                     /label=SH3 domain
     CDS             303..348
                     /label=linker
     CDS             349..1230
                     /label=membrane protein LGT
     misc_feature    1231..1233
                     /label=stop codon
     CDS             22..1233
                     /label=BBa_K1260002
     stem_loop       1253..1296
                     /label=stem_loop
     misc_feature    1242..1321
                     /label=BBa_B0010
     terminator      1242..1321
                     /label=BBa_B0010
     stem_loop       1337..1356
                     /label=T7 TE
     misc_feature    1363..1363
                     /label=stop
     polyA_site      1357..1370
                     /label=polya
     misc_feature    1330..1370
                     /label=BBa_B0012
     terminator      1330..1370
                     /label=BBa_B0012
     terminator      1242..1370
                     /label=BBa_B0015
ORIGIN
        1 attaaagagg agaaatacta gatgaaaaag atttggctgg cgctggctgg tttagtttta
       61 gcgtttagcg caagcgcagc tgaggccgcc gcaaaagaag cagcagctaa ggaagctgcg
      121 gcgaaggcag agtatgtgcg ggccctgttt gactttaatg ggaatgatga agaagacctt
      181 ccctttaaga aaggagacat cctgagaatc cgggataagc ctgaagagca gtggtggaat
      241 gcagaggaca gcgaaggaaa gagggggatg attcctgtcc cttacgtgga gaagtatact
      301 agagaggccg ccgcaaaaga agcagcagct aaggaagctg cggcgaagat gaccagtagc
      361 tatctgcatt ttccggagtt tgatccggtc attttctcaa taggacccgt ggcgcttcac
      421 tggtacggcc tgatgtatct ggtgggtttc atttttgcaa tgtggctggc aacacgacgg
      481 gcgaatcgtc cgggcagcgg ctggaccaaa aatgaagttg aaaacttact ctatgcgggc
      541 ttcctcggcg tcttcctcgg gggacgtatt ggttatgttc tgttctacaa tttcccgcag
      601 tttatggccg atccgctgta tctgttccgt gtctgggacg gcggcatgtc tttccacggc
      661 ggcctgattg gcgttatcgt ggtgatgatt atcttcgccc gccgtactaa acgttccttc
      721 ttccaggtct ctgattttat cgcaccactc attccgtttg gtcttggtgc cgggcgtctg
      781 ggcaacttta ttaacggtga attgtggggc cgcgttgacc cgaacttccc gtttgccatg
      841 ctgttccctg gctcccgtac agaagatatt ttgctgctgc aaaccaaccc gcagtggcaa
      901 tccattttcg acacttacgg tgtgctgccg cgccacccat cacagcttta cgagctgctg
      961 ctggaaggtg tggtgctgtt tattatcctc aacctgtata ttcgtaaacc acgcccaatg
     1021 ggagctgtct caggtttgtt cctgattggt tacggcgcgt ttcgcatcat tgttgagttt
     1081 ttccgccagc ccgacgcgca gtttaccggt gcctgggtgc agtacatcag catggggcaa
     1141 attctttcca tcccgatgat tgtcgcgggt gtgatcatga tggtctgggc atatcgtcgc
     1201 agcccacagc aacaggtttc cactagagct taatactaga gccaggcatc aaataaaacg
     1261 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1321 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//