BBa_K1260002
1
BBa_K1260002
membrane protein LGT with a SH3 domain
2014-05-22T11:00:00Z
2015-05-08T01:09:45Z
It is reconstructed from the BBa_K771007.
The gene has a membrane protein LGT and a SH3 domain. With the fusion protein it expressd on the E.coli's membrane, you can attach the proteins with a SH3 ligand on its tail.
false
false
_1618_
0
19163
9
It's complicated
false
The part is a little bit difficult to be PCR because of the same sequence of two linkers on it.
false
Zhengfeng Liu
annotation2378214
1
stop codon
range2378214
1
1210
1212
annotation2378210
1
start codon
range2378210
1
1
3
annotation2378212
1
linker
range2378212
1
282
327
annotation2378213
1
membrane protein LGT
range2378213
1
328
1209
annotation2378211
1
SH3 domain
range2378211
1
1
282
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_K1260010
1
BBa_K1260010
RBS 0030+ membrane protein LGT+double terminter
2014-06-07T11:00:00Z
2015-05-08T01:09:45Z
It comes from BBa_B0030 ,BBa_K1260002, BBa_B0015
It is a composite part constructed by BBa_K1260002, BBa_B0030 and BBa_B0015,
false
false
_1618_
0
19163
9
It's complicated
false
The RBS is in front of BBa_K1260002, the terminator is after BBa_K1260002
false
Zhengfeng Liu
component2378698
1
BBa_K1260002
component2378691
1
BBa_B0030
component2378705
1
BBa_B0015
annotation2378698
1
BBa_K1260002
range2378698
1
22
1233
annotation2378705
1
BBa_B0015
range2378705
1
1242
1370
annotation2378691
1
BBa_B0030
range2378691
1
1
15
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K1260002_sequence
1
atgaaaaagatttggctggcgctggctggtttagttttagcgtttagcgcaagcgcagctgaggccgccgcaaaagaagcagcagctaaggaagctgcggcgaaggcagagtatgtgcgggccctgtttgactttaatgggaatgatgaagaagaccttccctttaagaaaggagacatcctgagaatccgggataagcctgaagagcagtggtggaatgcagaggacagcgaaggaaagagggggatgattcctgtcccttacgtggagaagtatactagagaggccgccgcaaaagaagcagcagctaaggaagctgcggcgaagatgaccagtagctatctgcattttccggagtttgatccggtcattttctcaataggacccgtggcgcttcactggtacggcctgatgtatctggtgggtttcatttttgcaatgtggctggcaacacgacgggcgaatcgtccgggcagcggctggaccaaaaatgaagttgaaaacttactctatgcgggcttcctcggcgtcttcctcgggggacgtattggttatgttctgttctacaatttcccgcagtttatggccgatccgctgtatctgttccgtgtctgggacggcggcatgtctttccacggcggcctgattggcgttatcgtggtgatgattatcttcgcccgccgtactaaacgttccttcttccaggtctctgattttatcgcaccactcattccgtttggtcttggtgccgggcgtctgggcaactttattaacggtgaattgtggggccgcgttgacccgaacttcccgtttgccatgctgttccctggctcccgtacagaagatattttgctgctgcaaaccaacccgcagtggcaatccattttcgacacttacggtgtgctgccgcgccacccatcacagctttacgagctgctgctggaaggtgtggtgctgtttattatcctcaacctgtatattcgtaaaccacgcccaatgggagctgtctcaggtttgttcctgattggttacggcgcgtttcgcatcattgttgagtttttccgccagcccgacgcgcagtttaccggtgcctgggtgcagtacatcagcatggggcaaattctttccatcccgatgattgtcgcgggtgtgatcatgatggtctgggcatatcgtcgcagcccacagcaacaggtttccactagagcttaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1260010_sequence
1
attaaagaggagaaatactagatgaaaaagatttggctggcgctggctggtttagttttagcgtttagcgcaagcgcagctgaggccgccgcaaaagaagcagcagctaaggaagctgcggcgaaggcagagtatgtgcgggccctgtttgactttaatgggaatgatgaagaagaccttccctttaagaaaggagacatcctgagaatccgggataagcctgaagagcagtggtggaatgcagaggacagcgaaggaaagagggggatgattcctgtcccttacgtggagaagtatactagagaggccgccgcaaaagaagcagcagctaaggaagctgcggcgaagatgaccagtagctatctgcattttccggagtttgatccggtcattttctcaataggacccgtggcgcttcactggtacggcctgatgtatctggtgggtttcatttttgcaatgtggctggcaacacgacgggcgaatcgtccgggcagcggctggaccaaaaatgaagttgaaaacttactctatgcgggcttcctcggcgtcttcctcgggggacgtattggttatgttctgttctacaatttcccgcagtttatggccgatccgctgtatctgttccgtgtctgggacggcggcatgtctttccacggcggcctgattggcgttatcgtggtgatgattatcttcgcccgccgtactaaacgttccttcttccaggtctctgattttatcgcaccactcattccgtttggtcttggtgccgggcgtctgggcaactttattaacggtgaattgtggggccgcgttgacccgaacttcccgtttgccatgctgttccctggctcccgtacagaagatattttgctgctgcaaaccaacccgcagtggcaatccattttcgacacttacggtgtgctgccgcgccacccatcacagctttacgagctgctgctggaaggtgtggtgctgtttattatcctcaacctgtatattcgtaaaccacgcccaatgggagctgtctcaggtttgttcctgattggttacggcgcgtttcgcatcattgttgagtttttccgccagcccgacgcgcagtttaccggtgcctgggtgcagtacatcagcatggggcaaattctttccatcccgatgattgtcgcgggtgtgatcatgatggtctgggcatatcgtcgcagcccacagcaacaggtttccactagagcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z