LOCUS       BBa_K1260011            1508 bp    DNA     linear       22-Nov-2024
DEFINITION  pbad promoter and membrane protein LGT (whole device)
ACCESSION   BBa_K1260011
VERSION     BBa_K1260011.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206001
     RBS             139..153
                     /label=RBS-1\Strong
     RBS             146..150
                     /label=RBS
     misc_feature    139..153
                     /label=BBa_B0030
     RBS             139..153
                     /label=BBa_B0030
     misc_feature    160..162
                     /label=start codon
     CDS             160..441
                     /label=SH3 domain
     CDS             441..486
                     /label=linker
     CDS             487..1368
                     /label=membrane protein LGT
     misc_feature    1369..1371
                     /label=stop codon
     CDS             160..1371
                     /label=BBa_K1260002
     stem_loop       1391..1434
                     /label=stem_loop
     misc_feature    1380..1459
                     /label=BBa_B0010
     terminator      1380..1459
                     /label=BBa_B0010
     stem_loop       1475..1494
                     /label=T7 TE
     misc_feature    1501..1501
                     /label=stop
     polyA_site      1495..1508
                     /label=polya
     misc_feature    1468..1508
                     /label=BBa_B0012
     terminator      1468..1508
                     /label=BBa_B0012
     terminator      1380..1508
                     /label=BBa_B0015
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agctttttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagat taaagaggag aaatactaga tgaaaaagat ttggctggcg
      181 ctggctggtt tagttttagc gtttagcgca agcgcagctg aggccgccgc aaaagaagca
      241 gcagctaagg aagctgcggc gaaggcagag tatgtgcggg ccctgtttga ctttaatggg
      301 aatgatgaag aagaccttcc ctttaagaaa ggagacatcc tgagaatccg ggataagcct
      361 gaagagcagt ggtggaatgc agaggacagc gaaggaaaga gggggatgat tcctgtccct
      421 tacgtggaga agtatactag agaggccgcc gcaaaagaag cagcagctaa ggaagctgcg
      481 gcgaagatga ccagtagcta tctgcatttt ccggagtttg atccggtcat tttctcaata
      541 ggacccgtgg cgcttcactg gtacggcctg atgtatctgg tgggtttcat ttttgcaatg
      601 tggctggcaa cacgacgggc gaatcgtccg ggcagcggct ggaccaaaaa tgaagttgaa
      661 aacttactct atgcgggctt cctcggcgtc ttcctcgggg gacgtattgg ttatgttctg
      721 ttctacaatt tcccgcagtt tatggccgat ccgctgtatc tgttccgtgt ctgggacggc
      781 ggcatgtctt tccacggcgg cctgattggc gttatcgtgg tgatgattat cttcgcccgc
      841 cgtactaaac gttccttctt ccaggtctct gattttatcg caccactcat tccgtttggt
      901 cttggtgccg ggcgtctggg caactttatt aacggtgaat tgtggggccg cgttgacccg
      961 aacttcccgt ttgccatgct gttccctggc tcccgtacag aagatatttt gctgctgcaa
     1021 accaacccgc agtggcaatc cattttcgac acttacggtg tgctgccgcg ccacccatca
     1081 cagctttacg agctgctgct ggaaggtgtg gtgctgttta ttatcctcaa cctgtatatt
     1141 cgtaaaccac gcccaatggg agctgtctca ggtttgttcc tgattggtta cggcgcgttt
     1201 cgcatcattg ttgagttttt ccgccagccc gacgcgcagt ttaccggtgc ctgggtgcag
     1261 tacatcagca tggggcaaat tctttccatc ccgatgattg tcgcgggtgt gatcatgatg
     1321 gtctgggcat atcgtcgcag cccacagcaa caggtttcca ctagagctta atactagagc
     1381 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1441 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1501 cgtttata
//