LOCUS BBa_K1260012 2742 bp DNA linear 05-Nov-2024 DEFINITION AFB1-ScFv and membrane protein LGT whole device ACCESSION BBa_K1260012 VERSION BBa_K1260012.1 FEATURES Location/Qualifiers promoter 1..131 /label=promoter misc_feature 40..57 /label=AraI1 misc_feature 61..78 /label=AraI2 promoter 1..130 /label=BBa_K206001 RBS 139..153 /label=RBS-1\Strong RBS 146..150 /label=RBS misc_feature 139..153 /label=BBa_B0030 RBS 139..153 /label=BBa_B0030 misc_feature 160..162 /label=start misc_feature 160..228 /label=ssDsbA CDS 247..978 /label=AFB1-ScFv misc_feature 229..246 /label=linker 1 misc_feature 979..996 /label=6*his-tag misc_feature 1006..1050 /label=linker 2 misc_feature 1050..1083 /label=SH3 Ligand misc_feature 1084..1089 /label=stop CDS 160..1089 /label=BBa_K1260000 stem_loop 1109..1152 /label=stem_loop misc_feature 1098..1177 /label=BBa_B0010 terminator 1098..1177 /label=BBa_B0010 stem_loop 1193..1212 /label=T7 TE misc_feature 1219..1219 /label=stop polyA_site 1213..1226 /label=polya misc_feature 1186..1226 /label=BBa_B0012 terminator 1186..1226 /label=BBa_B0012 terminator 1098..1226 /label=BBa_B0015 misc_feature 1..1226 /label=BBa_K1260005 promoter 1235..1365 /label=promoter misc_feature 1274..1291 /label=AraI1 misc_feature 1295..1312 /label=AraI2 promoter 1235..1364 /label=BBa_K206001 RBS 1373..1387 /label=RBS-1\Strong RBS 1380..1384 /label=RBS misc_feature 1373..1387 /label=BBa_B0030 RBS 1373..1387 /label=BBa_B0030 misc_feature 1394..1396 /label=start codon CDS 1394..1675 /label=SH3 domain CDS 1675..1720 /label=linker CDS 1721..2602 /label=membrane protein LGT misc_feature 2603..2605 /label=stop codon CDS 1394..2605 /label=BBa_K1260002 stem_loop 2625..2668 /label=stem_loop misc_feature 2614..2693 /label=BBa_B0010 terminator 2614..2693 /label=BBa_B0010 stem_loop 2709..2728 /label=T7 TE misc_feature 2735..2735 /label=stop polyA_site 2729..2742 /label=polya misc_feature 2702..2742 /label=BBa_B0012 terminator 2702..2742 /label=BBa_B0012 terminator 2614..2742 /label=BBa_B0015 misc_feature 1235..2742 /label=BBa_K1260011 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agctttttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagat taaagaggag aaatactaga tgaaaaagac agctatcgcg 181 attgcagtgg cactggctgg tttcgctacc gtagcgcagg ccactagagg tagcggcagc 241 ggtagcacta gagcacaggt gaaactgcaa cagagcggaa ccgaagttgt taaaccgggg 301 gcaagcgtta agctgagctg taaggcaagc ggttatattt ttaccagcta tgatattgat 361 tgggttcgtc agaccccgga acagggtctg gaatggattg gttggatttt tccgggtgaa 421 ggtagcaccg aatataatga aaaattcaaa ggtcgtgcaa ccctgagcgt agataaaagc 481 agcagcaccg catatatgga actgaccagg ctgaccagcg aagatagcgc agtttatttt 541 tgtgcacgtg gtgattatta tcgtcgttat tttgacctgt ggggtcaggg taccaccgtt 601 accgtttcct caggtggagg cggttcaggc ggaggtggct ctggcggtgg cggatcggat 661 attgaactga cccagagccc ggcaattatg agcgcaagcc cgggtgaacg tgtcaccatg 721 acctgtagcg caagcagcag cattcgttat atttattggt atcagcagaa accgggtagc 781 agcccgcgtc tgctgattta tgatacaagc aatgttgcac cgggtgttcc ttttcgtttt 841 agcggtagcg gtagcggtac cagctatagc ctgaccatta atcgtatgga agcagaagat 901 gcagcaacct attattgtca ggaatggagc ggttatccgt acacctttgg tggtggtacc 961 aaactggaac tgaaacgtca tcatcatcat catcacacta gagctgaggc cgccgcaaaa 1021 gaagcagcag ctaaggaagc tgcggcgaag ccccctccag cgctgccgcc aaaacgtcgc 1081 cggtaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 1141 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 1201 ttcgggtggg cctttctgcg tttatatact agagacattg attatttgca cggcgtcaca 1261 ctttgctatg ccatagcttt tttatccata agattagcgg atcctacctg acgcttttta 1321 tcgcaactct ctactgtttc tccataccgt ttttttgggc tagctactag agattaaaga 1381 ggagaaatac tagatgaaaa agatttggct ggcgctggct ggtttagttt tagcgtttag 1441 cgcaagcgca gctgaggccg ccgcaaaaga agcagcagct aaggaagctg cggcgaaggc 1501 agagtatgtg cgggccctgt ttgactttaa tgggaatgat gaagaagacc ttccctttaa 1561 gaaaggagac atcctgagaa tccgggataa gcctgaagag cagtggtgga atgcagagga 1621 cagcgaagga aagaggggga tgattcctgt cccttacgtg gagaagtata ctagagaggc 1681 cgccgcaaaa gaagcagcag ctaaggaagc tgcggcgaag atgaccagta gctatctgca 1741 ttttccggag tttgatccgg tcattttctc aataggaccc gtggcgcttc actggtacgg 1801 cctgatgtat ctggtgggtt tcatttttgc aatgtggctg gcaacacgac gggcgaatcg 1861 tccgggcagc ggctggacca aaaatgaagt tgaaaactta ctctatgcgg gcttcctcgg 1921 cgtcttcctc gggggacgta ttggttatgt tctgttctac aatttcccgc agtttatggc 1981 cgatccgctg tatctgttcc gtgtctggga cggcggcatg tctttccacg gcggcctgat 2041 tggcgttatc gtggtgatga ttatcttcgc ccgccgtact aaacgttcct tcttccaggt 2101 ctctgatttt atcgcaccac tcattccgtt tggtcttggt gccgggcgtc tgggcaactt 2161 tattaacggt gaattgtggg gccgcgttga cccgaacttc ccgtttgcca tgctgttccc 2221 tggctcccgt acagaagata ttttgctgct gcaaaccaac ccgcagtggc aatccatttt 2281 cgacacttac ggtgtgctgc cgcgccaccc atcacagctt tacgagctgc tgctggaagg 2341 tgtggtgctg tttattatcc tcaacctgta tattcgtaaa ccacgcccaa tgggagctgt 2401 ctcaggtttg ttcctgattg gttacggcgc gtttcgcatc attgttgagt ttttccgcca 2461 gcccgacgcg cagtttaccg gtgcctgggt gcagtacatc agcatggggc aaattctttc 2521 catcccgatg attgtcgcgg gtgtgatcat gatggtctgg gcatatcgtc gcagcccaca 2581 gcaacaggtt tccactagag cttaatacta gagccaggca tcaaataaaa cgaaaggctc 2641 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 2701 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta //