##gff-version 3
##sequence-region BBa_K1260012 1 2742
BBa_K1260012	.	engineered_region	1235	2742	.	+	0	ID=BBa_K1260011;Name=BBa_K1260011
BBa_K1260012	.	CDS	1394	2605	.	+	0	ID=BBa_K1260002;Name=BBa_K1260002;Parent=BBa_K1260011
BBa_K1260012	.	CDS	1721	2602	.	+	0	ID=annotation2378213;Name=membrane protein LGT;Parent=BBa_K1260002
BBa_K1260012	.	CDS	1394	1675	.	+	0	ID=annotation2378211;Name=SH3 domain;Parent=BBa_K1260002
BBa_K1260012	.	stop_codon	2603	2605	.	+	0	ID=annotation2378214;Name=stop codon;Parent=BBa_K1260002
BBa_K1260012	.	start_codon	1394	1396	.	+	0	ID=annotation2378210;Name=start codon;Parent=BBa_K1260002
BBa_K1260012	.	CDS	1675	1720	.	+	0	ID=annotation2378212;Name=linker;Parent=BBa_K1260002
BBa_K1260012	.	terminator	2614	2742	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1260011
BBa_K1260012	.	terminator	2614	2693	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1260012	.	engineered_region	2614	2693	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1260012	.	stem_loop	2625	2668	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1260012	.	terminator	2702	2742	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1260012	.	stem_loop	2709	2728	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1260012	.	engineered_region	2702	2742	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1260012	.	polyA_site	2729	2742	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1260012	.	stop_codon	2735	2735	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1260012	.	ribosome_entry_site	1373	1387	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K1260011
BBa_K1260012	.	engineered_region	1373	1387	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1260012	.	ribosome_entry_site	1380	1384	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1260012	.	ribosome_entry_site	1373	1387	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1260012	.	promoter	1235	1364	.	+	0	ID=BBa_K206001;Name=BBa_K206001;Parent=BBa_K1260011
BBa_K1260012	.	operator	1295	1312	.	+	0	ID=annotation2049257;Name=AraI2;Parent=BBa_K206001
BBa_K1260012	.	operator	1274	1291	.	+	0	ID=annotation2049256;Name=AraI1;Parent=BBa_K206001
BBa_K1260012	.	promoter	1235	1365	.	+	0	ID=annotation2049255;Name=promoter;Parent=BBa_K206001
BBa_K1260012	.	engineered_region	1	1226	.	+	0	ID=BBa_K1260005;Name=BBa_K1260005
BBa_K1260012	.	terminator	1098	1226	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1260005
BBa_K1260012	.	terminator	1098	1177	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1260012	.	engineered_region	1098	1177	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1260012	.	stem_loop	1109	1152	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1260012	.	terminator	1186	1226	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1260012	.	stem_loop	1193	1212	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1260012	.	engineered_region	1186	1226	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1260012	.	polyA_site	1213	1226	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1260012	.	stop_codon	1219	1219	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1260012	.	ribosome_entry_site	139	153	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K1260005
BBa_K1260012	.	engineered_region	139	153	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1260012	.	ribosome_entry_site	146	150	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1260012	.	ribosome_entry_site	139	153	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1260012	.	promoter	1	130	.	+	0	ID=BBa_K206001;Name=BBa_K206001;Parent=BBa_K1260005
BBa_K1260012	.	operator	61	78	.	+	0	ID=annotation2049257;Name=AraI2;Parent=BBa_K206001
BBa_K1260012	.	operator	40	57	.	+	0	ID=annotation2049256;Name=AraI1;Parent=BBa_K206001
BBa_K1260012	.	promoter	1	131	.	+	0	ID=annotation2049255;Name=promoter;Parent=BBa_K206001
BBa_K1260012	.	CDS	160	1089	.	+	0	ID=BBa_K1260000;Name=BBa_K1260000;Parent=BBa_K1260005
BBa_K1260012	.	sequence_feature	1050	1083	.	+	0	ID=annotation2372759;Name=SH3 Ligand;Parent=BBa_K1260000
BBa_K1260012	.	start_codon	160	162	.	+	0	ID=annotation2372753;Name=start;Parent=BBa_K1260000
BBa_K1260012	.	tag	979	996	.	+	0	ID=annotation2372757;Name=6*his-tag;Parent=BBa_K1260000
BBa_K1260012	.	CDS	247	978	.	+	0	ID=annotation2372755;Name=AFB1-ScFv;Parent=BBa_K1260000
BBa_K1260012	.	sequence_feature	1006	1050	.	+	0	ID=annotation2372758;Name=linker 2;Parent=BBa_K1260000
BBa_K1260012	.	stop_codon	1084	1089	.	+	0	ID=annotation2372760;Name=stop;Parent=BBa_K1260000
BBa_K1260012	.	sequence_feature	229	246	.	+	0	ID=annotation2372756;Name=linker 1;Parent=BBa_K1260000
BBa_K1260012	.	sequence_feature	160	228	.	+	0	ID=annotation2372754;Name=ssDsbA;Parent=BBa_K1260000
>BBa_K1260012
acattgattatttgcacggcgtcacactttgctatgccatagcttttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagattaaagaggagaaatacta
gatgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggccactagaggtagcggc
agcggtagcactagagcacaggtgaaactgcaacagagcggaaccgaagttgttaaaccgggggcaagcgttaagctga
gctgtaaggcaagcggttatatttttaccagctatgatattgattgggttcgtcagaccccggaacagggtctggaatg
gattggttggatttttccgggtgaaggtagcaccgaatataatgaaaaattcaaaggtcgtgcaaccctgagcgtagat
aaaagcagcagcaccgcatatatggaactgaccaggctgaccagcgaagatagcgcagtttatttttgtgcacgtggtg
attattatcgtcgttattttgacctgtggggtcagggtaccaccgttaccgtttcctcaggtggaggcggttcaggcgg
aggtggctctggcggtggcggatcggatattgaactgacccagagcccggcaattatgagcgcaagcccgggtgaacgt
gtcaccatgacctgtagcgcaagcagcagcattcgttatatttattggtatcagcagaaaccgggtagcagcccgcgtc
tgctgatttatgatacaagcaatgttgcaccgggtgttccttttcgttttagcggtagcggtagcggtaccagctatag
cctgaccattaatcgtatggaagcagaagatgcagcaacctattattgtcaggaatggagcggttatccgtacaccttt
ggtggtggtaccaaactggaactgaaacgtcatcatcatcatcatcacactagagctgaggccgccgcaaaagaagcag
cagctaaggaagctgcggcgaagccccctccagcgctgccgccaaaacgtcgccggtaataatactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacacttt
gctatgccatagcttttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctcc
ataccgtttttttgggctagctactagagattaaagaggagaaatactagatgaaaaagatttggctggcgctggctgg
tttagttttagcgtttagcgcaagcgcagctgaggccgccgcaaaagaagcagcagctaaggaagctgcggcgaaggca
gagtatgtgcgggccctgtttgactttaatgggaatgatgaagaagaccttccctttaagaaaggagacatcctgagaa
tccgggataagcctgaagagcagtggtggaatgcagaggacagcgaaggaaagagggggatgattcctgtcccttacgt
ggagaagtatactagagaggccgccgcaaaagaagcagcagctaaggaagctgcggcgaagatgaccagtagctatctg
cattttccggagtttgatccggtcattttctcaataggacccgtggcgcttcactggtacggcctgatgtatctggtgg
gtttcatttttgcaatgtggctggcaacacgacgggcgaatcgtccgggcagcggctggaccaaaaatgaagttgaaaa
cttactctatgcgggcttcctcggcgtcttcctcgggggacgtattggttatgttctgttctacaatttcccgcagttt
atggccgatccgctgtatctgttccgtgtctgggacggcggcatgtctttccacggcggcctgattggcgttatcgtgg
tgatgattatcttcgcccgccgtactaaacgttccttcttccaggtctctgattttatcgcaccactcattccgtttgg
tcttggtgccgggcgtctgggcaactttattaacggtgaattgtggggccgcgttgacccgaacttcccgtttgccatg
ctgttccctggctcccgtacagaagatattttgctgctgcaaaccaacccgcagtggcaatccattttcgacacttacg
gtgtgctgccgcgccacccatcacagctttacgagctgctgctggaaggtgtggtgctgtttattatcctcaacctgta
tattcgtaaaccacgcccaatgggagctgtctcaggtttgttcctgattggttacggcgcgtttcgcatcattgttgag
tttttccgccagcccgacgcgcagtttaccggtgcctgggtgcagtacatcagcatggggcaaattctttccatcccga
tgattgtcgcgggtgtgatcatgatggtctgggcatatcgtcgcagcccacagcaacaggtttccactagagcttaata
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata