LOCUS       BBa_K127001              939 bp    DNA     linear       22-Nov-2024
DEFINITION  constitutive GFP generator with J23102 and B0034
ACCESSION   BBa_K127001
VERSION     BBa_K127001.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23102
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     RBS             64..76
                     /label=RBS-3\Weak
     RBS             70..73
                     /label=RBS
     misc_feature    64..76
                     /label=BBa_B0032
     RBS             64..76
                     /label=BBa_B0032
     CDS             83..802
                     /label=GFP protein
     CDS             83..802
                     /label=BBa_E0040
     stem_loop       822..865
                     /label=stem_loop
     misc_feature    811..890
                     /label=BBa_B0010
     terminator      811..890
                     /label=BBa_B0010
     stem_loop       906..925
                     /label=T7 TE
     misc_feature    932..932
                     /label=stop
     polyA_site      926..939
                     /label=polya
     misc_feature    899..939
                     /label=BBa_B0012
     terminator      899..939
                     /label=BBa_B0012
ORIGIN
        1 ttgacagcta gctcagtcct aggtactgtg ctagctacta gagaaagagg agaaatacta
       61 gagtcacaca ggaaagtact agatgcgtaa aggagaagaa cttttcactg gagttgtccc
      121 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
      181 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
      241 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag
      301 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
      361 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
      421 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
      481 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
      541 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga
      601 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
      661 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
      721 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
      781 ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt
      841 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      901 acactggctc accttcgggt gggcctttct gcgtttata
//