LOCUS BBa_K127003 2668 bp DNA linear 29-Nov-2024 DEFINITION Polystyrene binding peptide inserted CPX generated by qurum sensing and constitutive GFP genarator ACCESSION BBa_K127003 VERSION BBa_K127003.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 terminator 870..998 /label=BBa_B0015 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1..1061 /label=BBa_F2620 misc_feature 1074..1077 /label=conserved RBS 1070..1081 /label=BBa_B0034 misc_feature 1088..1090 /label=Start codon CDS 1088..1156 /label=Native Starting Signal misc_feature 1157..1171 /label=SfiI Restriction Site CDS 1178..1216 /label=Polystyrene Sticky Peptide misc_feature 1262..1279 /label=Linker CDS 1280..1564 /label=S54-F148 of OmpX misc_feature 1565..1576 /label=Linker CDS 1577..1735 /label=A1-S53 of OmpX misc_feature 1736..1741 /label=Double stop codon (TGATAA) misc_feature 1172..1177 /label=BglII Cut Site misc_feature 1229..1261 /label=T7 Antibody Tag misc_feature 1217..1222 /label=SalI Restriction Site CDS 1223..1228 /label=Trypsin Cleavage Site CDS 1088..1741 /label=Coding sequence CDS 1088..1741 /label=BBa_I728500 misc_feature 1..1741 /label=BBa_I728978 promoter 1750..1784 /label=BBa_J23102 RBS 1793..1805 /label=RBS-3\Weak RBS 1799..1802 /label=RBS misc_feature 1793..1805 /label=BBa_B0032 RBS 1793..1805 /label=BBa_B0032 CDS 1812..2531 /label=GFP protein CDS 1812..2531 /label=BBa_E0040 stem_loop 2551..2594 /label=stem_loop misc_feature 2540..2619 /label=BBa_B0010 terminator 2540..2619 /label=BBa_B0010 stem_loop 2635..2654 /label=T7 TE misc_feature 2661..2661 /label=stop polyA_site 2655..2668 /label=polya misc_feature 2628..2668 /label=BBa_B0012 terminator 2628..2668 /label=BBa_B0012 misc_feature 1793..2668 /label=BBa_E0240 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa 1081 atactagatg aaaaaaattg cgtgcctgag cgcgctggcc gcggttctgg cctttaccgc 1141 gggcaccagc gttgcgggcc agtctggcca aagatctttt ttcagctttt tttttccggc 1201 gagcgcgtgg ggtagcgtcg acaaacgtat ggcgagcatg accggcggtc agcagatggg 1261 tggtggccag agcggccaga gcggtgatta taacaaaaac cagtattatg gcattaccgc 1321 gggtccggcg tatcgtatta acgattgggc gagcatttat ggcgtggtgg gcgtgggcta 1381 tggcaaattt cagaccaccg aatatccgac ctataaacac gataccagcg attatggctt 1441 tagctatggc gcgggtctgc aatttaatcc gatggaaaac gtggcgctgg attttagcta 1501 tgaacagagc cgtattcgta gcgtggatgt gggcacctgg attgcgggcg tgggttatcg 1561 ttttggcggc agcggtgcga ccagcaccgt gaccggcggt tatgcgcaga gcgatgcgca 1621 gggccagatg aacaaaatgg gcggcttcaa cctgaaatat cgctatgaag aagataacag 1681 cccgctgggc gtgattggca gctttaccta taccgaaaaa agccgtaccg cgagctgata 1741 atactagagt tgacagctag ctcagtccta ggtactgtgc tagctactag agtcacacag 1801 gaaagtacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg 1861 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg 1921 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat 1981 ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc 2041 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa 2101 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg 2161 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc 2221 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac 2281 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc 2341 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag 2401 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc 2461 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat 2521 acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 2581 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 2641 ccttcgggtg ggcctttctg cgtttata //