##gff-version 3
##sequence-region BBa_K127007 1 2007
BBa_K127007	.	engineered_region	807	2007	.	+	0	ID=BBa_I732910;Name=BBa_I732910
BBa_K127007	.	engineered_region	944	1987	.	+	0	ID=BBa_J09855;Name=BBa_J09855;Parent=BBa_I732910
BBa_K127007	.	promoter	944	980	.	+	0	ID=BBa_I14032;Name=BBa_I14032;Parent=BBa_J09855
BBa_K127007	.	operator	944	980	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_K127007	.	promoter	946	951	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_K127007	.	promoter	969	974	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_K127007	.	promoter	1933	1987	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J09855
BBa_K127007	.	engineered_region	1933	1987	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K127007	.	promoter	1952	1957	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K127007	.	promoter	1974	1979	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K127007	.	start_codon	1985	1985	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K127007	.	non_covalent_binding_site	1933	1952	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K127007	.	engineered_region	989	1924	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_J09855
BBa_K127007	.	terminator	1796	1875	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K127007	.	stem_loop	1807	1850	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K127007	.	engineered_region	1796	1875	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K127007	.	terminator	1884	1924	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K127007	.	polyA_site	1911	1924	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K127007	.	engineered_region	1884	1924	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K127007	.	stem_loop	1891	1910	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K127007	.	stop_codon	1917	1917	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K127007	.	ribosome_entry_site	989	1000	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K127007	.	conserved_region	993	996	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K127007	.	CDS	1007	1762	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K127007	.	CDS	1007	1756	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K127007	.	engineered_tag	1763	1787	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K127007	.	engineered_region	1007	1762	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K127007	.	point_mutation	1180	1180	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K127007	.	engineered_region	1007	1008	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K127007	.	point_mutation	1498	1498	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K127007	.	terminator	807	935	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I732910
BBa_K127007	.	terminator	895	935	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K127007	.	polyA_site	922	935	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K127007	.	engineered_region	895	935	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K127007	.	stem_loop	902	921	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K127007	.	stop_codon	928	928	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K127007	.	terminator	807	886	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K127007	.	stem_loop	818	861	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K127007	.	engineered_region	807	886	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K127007	.	ribosome_entry_site	1996	2007	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732910
BBa_K127007	.	conserved_region	2000	2003	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K127007	.	CDS	19	636	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_K127007	.	CDS	19	597	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K127007	.	engineered_region	19	636	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K127007	.	tag	598	629	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K127007	.	engineered_tag	637	661	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K127007	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K127007	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K127007	.	terminator	670	798	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K127007	.	terminator	758	798	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K127007	.	polyA_site	785	798	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K127007	.	engineered_region	758	798	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K127007	.	stem_loop	765	784	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K127007	.	stop_codon	791	791	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K127007	.	terminator	670	749	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K127007	.	stem_loop	681	724	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K127007	.	engineered_region	670	749	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K127007
aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggta
ttctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatga
gtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacct
acaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatag
tcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatt
tgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgattt
ttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtat
tgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagctta
ataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtggtg
caaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatgccgac
gacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgacta
aaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctaga
taattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaac
tccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaag
cgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgc
acattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctcta
gttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttag
cgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccattt
aaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgat
tgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgtta
tagtcgaataaatactagagaaagaggagaaa