LOCUS BBa_K127008 2050 bp DNA linear 15-Dec-2024 DEFINITION J23102 B0034 C0061 B0015 I732910 ACCESSION BBa_K127008 VERSION BBa_K127008.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23102 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 641..672 /label=LVA CDS 62..640 /label=luxI misc_feature 62..679 /label=BBa_C0061 misc_feature 680..704 /label=Help:Barcodes CDS 62..679 /label=BBa_C0061 stem_loop 724..767 /label=stem_loop misc_feature 713..792 /label=BBa_B0010 terminator 713..792 /label=BBa_B0010 stem_loop 808..827 /label=T7 TE misc_feature 834..834 /label=stop polyA_site 828..841 /label=polya misc_feature 801..841 /label=BBa_B0012 terminator 801..841 /label=BBa_B0012 terminator 713..841 /label=BBa_B0015 stem_loop 861..904 /label=stem_loop misc_feature 850..929 /label=BBa_B0010 terminator 850..929 /label=BBa_B0010 stem_loop 945..964 /label=T7 TE misc_feature 971..971 /label=stop polyA_site 965..978 /label=polya misc_feature 938..978 /label=BBa_B0012 terminator 938..978 /label=BBa_B0012 terminator 850..978 /label=BBa_B0015 misc_feature 987..1023 /label=P(Lac) IQ promoter 989..994 /label=-35 promoter 1012..1017 /label=-10 promoter 987..1023 /label=BBa_I14032 misc_feature 1036..1039 /label=conserved RBS 1032..1043 /label=BBa_B0034 misc_feature 1050..1051 /label=prefix misc_feature 1223..1223 /label=T misc_feature 1541..1541 /label=A CDS 1050..1799 /label=luxR misc_feature 1050..1805 /label=BBa_C0062 misc_feature 1806..1830 /label=Help:Barcodes CDS 1050..1805 /label=BBa_C0062 stem_loop 1850..1893 /label=stem_loop misc_feature 1839..1918 /label=BBa_B0010 terminator 1839..1918 /label=BBa_B0010 stem_loop 1934..1953 /label=T7 TE misc_feature 1960..1960 /label=stop polyA_site 1954..1967 /label=polya misc_feature 1927..1967 /label=BBa_B0012 terminator 1927..1967 /label=BBa_B0012 misc_feature 1032..1967 /label=BBa_I0462 misc_feature 1976..1995 /label=LuxR/HSL promoter 1995..2000 /label=-35 promoter 2017..2022 /label=-10 misc_feature 2028..2028 /label=start misc_feature 1976..2030 /label=BBa_R0062 promoter 1976..2030 /label=BBa_R0062 misc_feature 987..2030 /label=BBa_J09855 misc_feature 2043..2046 /label=conserved RBS 2039..2050 /label=BBa_B0034 misc_feature 850..2050 /label=BBa_I732910 ORIGIN 1 ttgacagcta gctcagtcct aggtactgtg ctagctacta gagaaagagg agaaatacta 61 gatgactata atgataaaaa aatcggattt tttggcaatt ccatcggagg agtataaagg 121 tattctaagt cttcgttatc aagtgtttaa gcaaagactt gagtgggact tagttgtaga 181 aaataacctt gaatcagatg agtatgataa ctcaaatgca gaatatattt atgcttgtga 241 tgatactgaa aatgtaagtg gatgctggcg tttattacct acaacaggtg attatatgct 301 gaaaagtgtt tttcctgaat tgcttggtca acagagtgct cccaaagatc ctaatatagt 361 cgaattaagt cgttttgctg taggtaaaaa tagctcaaag ataaataact ctgctagtga 421 aattacaatg aaactatttg aagctatata taaacacgct gttagtcaag gtattacaga 481 atatgtaaca gtaacatcaa cagcaataga gcgattttta aagcgtatta aagttccttg 541 tcatcgtatt ggagacaaag aaattcatgt attaggtgat actaaatcgg ttgtattgtc 601 tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat gctgcaaacg acgaaaacta 661 cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag agccaggcat 721 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 781 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 841 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 961 ggcctttctg cgtttatata ctagagtggt gcaaaacctt tcgcggtatg gcatgatagc 1021 gcctactaga gaaagaggag aaatactaga tgaaaaacat aaatgccgac gacacataca 1081 gaataattaa taaaattaaa gcttgtagaa gcaataatga tattaatcaa tgcttatctg 1141 atatgactaa aatggtacat tgtgaatatt atttactcgc gatcatttat cctcattcta 1201 tggttaaatc tgatatttca atcctagata attaccctaa aaaatggagg caatattatg 1261 atgacgctaa tttaataaaa tatgatccta tagtagatta ttctaactcc aatcattcac 1321 caattaattg gaatatattt gaaaacaatg ctgtaaataa aaaatctcca aatgtaatta 1381 aagaagcgaa aacatcaggt cttatcactg ggtttagttt ccctattcat acggctaaca 1441 atggcttcgg aatgcttagt tttgcacatt cagaaaaaga caactatata gatagtttat 1501 ttttacatgc gtgtatgaac ataccattaa ttgttccttc tctagttgat aattatcgaa 1561 aaataaatat agcaaataat aaatcaaaca acgatttaac caaaagagaa aaagaatgtt 1621 tagcgtgggc atgcgaagga aaaagctctt gggatatttc aaaaatatta ggttgcagtg 1681 agcgtactgt cactttccat ttaaccaatg cgcaaatgaa actcaataca acaaaccgct 1741 gccaaagtat ttctaaagca attttaacag gagcaattga ttgcccatac tttaaaaatt 1801 aataacactg atagtgctag tgtagatcac tactagagcc aggcatcaaa taaaacgaaa 1861 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 1921 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagacctg 1981 taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataaa tactagagaa 2041 agaggagaaa //