##gff-version 3
##sequence-region BBa_K127009 1 2050
BBa_K127009	.	terminator	713	841	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K127009	.	terminator	713	792	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K127009	.	engineered_region	713	792	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K127009	.	stem_loop	724	767	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K127009	.	terminator	801	841	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K127009	.	stop_codon	834	834	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K127009	.	polyA_site	828	841	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K127009	.	stem_loop	808	827	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K127009	.	engineered_region	801	841	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K127009	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K127009	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K127009	.	CDS	62	679	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_K127009	.	CDS	62	640	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K127009	.	engineered_tag	680	704	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K127009	.	engineered_region	62	679	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K127009	.	tag	641	672	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K127009	.	promoter	1	35	.	+	0	ID=BBa_J23105;Name=BBa_J23105
BBa_K127009	.	engineered_region	850	2050	.	+	0	ID=BBa_I732910;Name=BBa_I732910
BBa_K127009	.	engineered_region	987	2030	.	+	0	ID=BBa_J09855;Name=BBa_J09855;Parent=BBa_I732910
BBa_K127009	.	engineered_region	1032	1967	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_J09855
BBa_K127009	.	terminator	1927	1967	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K127009	.	stop_codon	1960	1960	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K127009	.	polyA_site	1954	1967	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K127009	.	stem_loop	1934	1953	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K127009	.	engineered_region	1927	1967	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K127009	.	CDS	1050	1805	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K127009	.	engineered_tag	1806	1830	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K127009	.	engineered_region	1050	1805	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K127009	.	engineered_region	1050	1051	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K127009	.	point_mutation	1223	1223	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K127009	.	CDS	1050	1799	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K127009	.	point_mutation	1541	1541	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K127009	.	terminator	1839	1918	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K127009	.	engineered_region	1839	1918	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K127009	.	stem_loop	1850	1893	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K127009	.	ribosome_entry_site	1032	1043	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K127009	.	conserved_region	1036	1039	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K127009	.	promoter	1976	2030	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J09855
BBa_K127009	.	start_codon	2028	2028	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K127009	.	promoter	2017	2022	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K127009	.	promoter	1995	2000	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K127009	.	engineered_region	1976	2030	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K127009	.	non_covalent_binding_site	1976	1995	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K127009	.	promoter	987	1023	.	+	0	ID=BBa_I14032;Name=BBa_I14032;Parent=BBa_J09855
BBa_K127009	.	promoter	1012	1017	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_K127009	.	promoter	989	994	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_K127009	.	operator	987	1023	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_K127009	.	terminator	850	978	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I732910
BBa_K127009	.	terminator	850	929	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K127009	.	engineered_region	850	929	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K127009	.	stem_loop	861	904	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K127009	.	terminator	938	978	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K127009	.	stop_codon	971	971	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K127009	.	polyA_site	965	978	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K127009	.	stem_loop	945	964	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K127009	.	engineered_region	938	978	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K127009	.	ribosome_entry_site	2039	2050	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732910
BBa_K127009	.	conserved_region	2043	2046	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K127009
tttacggctagctcagtcctaggtactatgctagctactagagaaagaggagaaatactagatgactataatgataaaa
aaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagac
ttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttg
tgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcct
gaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagct
caaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtat
tacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattgga
gacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcag
tcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagtggtgcaaaacctttcgcggtatggcatgatagcgcctact
agagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgt
agaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatca
tttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatga
cgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaa
aacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttcc
ctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatt
tttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataat
aaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatattt
caaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccg
ctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgc
tagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaa