BBa_K128008 1 BBa_K128008 pJK650 plasmid 2008-10-29T12:00:00Z 2015-05-08T01:09:46Z Donated by Pascale Serror, IRNA, in France Expression vector pJK650 from ''L. delbruckii'' bacteria. Easiest if the plasmid is grown up in ''Lactococcus lactis'' and extracted from there. Confers Erythromycin resistance; select in ''Lactococcus lactis'' with 5 ug/ml Em, select in ''Lactobacillus'' with 7.5 ug/ml Em. With electroporation procedures reported by Serror et al. (App. and Env. Microbiology 2002), pJK650 is compatible with ''Lactobacillus delbruckii'' subsp. ''bulgaricus'' strains '''VI104''' and '''ATCC 11842''' and ''Lactobacillus delbruckii'' subsp. ''lactis'' strain '''ATCC 10697'''. Transformation efficiency ~ 10^2 transformants/ug. For detailed electroporation protocols, please visit the MIT 2008 iGEM wiki or the ''Lactobacillus'' protocols section on openwetware.org false false _178_ 0 2825 9 Not in stock false Compatible with only a few strains of Lactobacillus. Keep this in mind when attempting to use pJK650, since Lactobacillus can be picky with its range of plasmids false Derek Ju BBa_K128008_sequence 1 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igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z