BBa_K129004 1 BBa_K129004 LamB_HP-metal binding domain in LamB-153 residue 2008-11-08T12:00:00Z 2015-05-08T01:09:46Z To make HP_LamB GATCCAGCTGGTCATCATCCACACGGTGCT is inserted in LamB protein at 153. aa residue encodes the Ala-Gly-His-His-Pro-His-Gly-Ala-C sequence, so LamB has source and this gene have to be replaced HP domain in 153 residu DEFINITION E.coli lamB gene fragment. ACCESSION A31943 VERSION A31943.1 GI:1567229 KEYWORDS . SOURCE Escherichia coli ORGANISM Escherichia coli Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia. LamB tolerates insertions of long heterologous peptides at a permissive loop (between structural codons 153 and 154) exposed to the external medium without a loss of function.protein. The histidine-rich sequence Gly-His-His-Pro-His-Gly employed in LamB and called HP. HP represents one to three multiple repeats along the C-terminal part of the human plasma metal transport protein known as the histidine rich-glycoprotein(HRG. The HRG binds heme and various divalent heavy metal ions with the following apparent order of affinity: Cu21; Hg21 . Zn21 . Ni21 . Cd21 . Co21 . The HP sequence is believed to form surface metal binding sites (MBSs) of HRG, and it has been also successfully used to immobilize Cu21 and Zn21 on IMAC columns. false false _236_ 0 2526 9 Not in stock false GATCCAGCTGGTCATCATCCACACGGTGCT is inserted in LamB-153 residue because permissive loop (between structural codons 153and 154) can be exposed to the external medium without a loss of function. false Burak YILMAZ BBa_K129004_sequence 1 gaattcgcggccgcttctagaggttgatttccacggctatgcacgttccggtattggttggacaggtagcggcggtgaacaacagtgtttccagactaccggtgctcaaagtaaataccgtcttggcaacgaatgtgaaacttatgctgaattaaaattgggtcaggaagtgtggaaagagggcgataagagcttctatttcgacactaacgtggcctattccgtcgcacaacagaatgactgggaagctaccgatccggccttccgtgaagcaaacgtgcagggtaaaaacctgatcgaatggctgccaggctccaccatctgggcaggtaagcgcttctaccaacgtcatgacgttcatatgatcgacttctactactgggatatttctggtcctggtgccggtctggaaaacatcgatgttggcttcggtaaactctctctggcagcaacccgctcctctgaagctggtggttcttccccagctggtcatcatccacacggtgctgattctttcgccagcaacaatatttatgactataccaacgaaaccgcgaacgacgttttcgatgtgcgtttagcgcagatggaaatcaacccgggcggcacattagaactgggtgtcgactacggtcgtgccaacttgcgtgataactatcgtctggttgatggcgcatcgaaagacggctggttattcactgctgaacatactcagagtgtcctgaagggctttaacaagtttgttgttcagtacgctactgactcgatgacctcgcagggtaaagggctgtcgcagggttctggcgttgcatttgataacgaaaaatttgcctacaatatcaacaacaacggtcacatgctgcgtatcctcgaccacggtgcgatctccatgggcgacaactgggacatgatgtacgtgggtatgtaccaggatatcaactgggataacgacaacggcaccaagtggtggaccgtcggtattcgcccgatgtacaagtggacgccaatcatgagcaccgtgatggaaatcggctacgacaacgtcgaatcccagcgcaccggcgacaagaacaatcagtacaaaattaccctcgcacaacaatggcaggctggcgacagcatctggtcacgcccggctattcgtgtcttcgcaacctacgccaagtgggatgagaaatggggttacgactacaccggtaacgctgataacaacgcgaacttcggcaaagccgttcctgctgatttcaacggcggcagcttcggtcgtggcgacagcgacgagtggaccttcggtgcccagatggaaatctggtggtactagtagcggccgctgcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z