BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1291571
1
BBa_K1291571
HORSERADISH PEROXIDASE TYPE C
2014-06-22T11:00:00Z
2015-05-08T01:09:46Z
HORSERADISH
We have synthesized this gene de novo.
The enzyme horseradish peroxidase (HRP), found in the roots of plant horseradish, is used extensively in biochemistry applications primarily for its ability to amplify a weak signal and increase detectability of a target molecule. It is a metalloenzyme with many isoforms and the most studied type is C.
This enzyme is primerly responsible for the degradation of industrial dyes such as Methylene Blue.
false
false
_1654_
0
11153
9
Not in stock
true
We have removed the restriction sites of EcoRI, PstI, SpeI, XbaI manually.
false
İBRAHİM YASİR ORHAN
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_K1291071
1
BBa_K1291071
HORSERADISH PEROXIDASE TYPE C COMPOSITE PART
2014-06-22T11:00:00Z
2015-05-08T01:09:46Z
Horseradish peroxidase Type C region was taken from the horseradish and the other compositions were synthetically merged with this sequence.
The enzyme horseradish peroxidase (HRP), found in the roots of plant horseradish, is used extensively in biochemistry applications primarily for its ability to amplify a weak signal and increase detectability of a target molecule. It is a metalloenzyme with many isoforms and the most studied type is C. This enzyme is primerly responsible for the degradation of industrial dyes such as Methylene Blue.
The composite part starts off with the J23119 constitutive promoter, continues with the RBS which is followed by the Horseradish peroxidase coding region. The sequence ends with the terminators which come after the Histag region. There is the BamH1 enzyme restriction cite on the sequence before the RBS.
false
false
_1654_
0
11153
9
Not in stock
false
All EcoRI, PstI, SpeI, XbaI restriction sites were removed manually and BamH1 restriction cites were placed at the start and at the end of the composite part.
false
İBRAHİM YASİR ORHAN
component2379468
1
BBa_K1291571
component2379467
1
BBa_B0034
component2379465
1
BBa_J23119
annotation2379468
1
BBa_K1291571
range2379468
1
62
1120
annotation2379465
1
BBa_J23119
range2379465
1
1
35
annotation2379467
1
BBa_B0034
range2379467
1
44
55
BBa_K1291071_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgcatttcagcagcagcagcaccctgtttacctgtattaccctgattccgctggtttgtctgattctgcatgcaagcctgagcgatgcacagctgaccccgacctttattgataatagctgtccgaatgttagcaatattgttcgtgataccattgttaatgaactgcgtagcgacccgcgtattgcagcaagcattctgcgtctgcattttcatgattgttttgttaatggttgtgatgcaagtattctgctggataataccaccagctttcgtaccgaaaaagatgcatttggtaatgcaaatagcgcacgtggttttccggttattgatcgtatgaaagcagccgttgaaagcgcatgtccgcgtaccgttagctgtgcagatctgctgaccattgcagcacagcagagcgttaccctggcaggtggtccgagctggcgtgttccgctgggtcgtcgtgatagcttacaagcatttttggatctggcaaatgcaaatctgccggcaccgttttttaccctgccgcagctgaaagatagctttcgtaatgttggtctgaatcgtagcagcgatctggttgcactgagcggtggtcatacctttggtaaaaatcagtgtcgttttattatggatcgtctgtataattttagcaataccggtctgcctgacccgaccctgaataccacctatttacaaaccctgcgtggtctgtgtccgctgaatggtaatctgagcgcattggttgattttgatctgcgtaccccgaccatttttgataataaatattatgttaatttggaagaacagaaaggtctgattcagagcgatcaggaactgtttagcagcccgaatgcaaccgataccattccgttggttcgtagctttgcaaatagcacccagaccttttttaatgcatttgttgaagcaatggatcgtatgggcaatattaccccgctgaccggtacccagggtcagattcgtctgaattgtcgtgttgttaatagcaatagcctgctgcatgatatggttgaagttgttgattttgttagcagcatg
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1291571_sequence
1
atgcatttcagcagcagcagcaccctgtttacctgtattaccctgattccgctggtttgtctgattctgcatgcaagcctgagcgatgcacagctgaccccgacctttattgataatagctgtccgaatgttagcaatattgttcgtgataccattgttaatgaactgcgtagcgacccgcgtattgcagcaagcattctgcgtctgcattttcatgattgttttgttaatggttgtgatgcaagtattctgctggataataccaccagctttcgtaccgaaaaagatgcatttggtaatgcaaatagcgcacgtggttttccggttattgatcgtatgaaagcagccgttgaaagcgcatgtccgcgtaccgttagctgtgcagatctgctgaccattgcagcacagcagagcgttaccctggcaggtggtccgagctggcgtgttccgctgggtcgtcgtgatagcttacaagcatttttggatctggcaaatgcaaatctgccggcaccgttttttaccctgccgcagctgaaagatagctttcgtaatgttggtctgaatcgtagcagcgatctggttgcactgagcggtggtcatacctttggtaaaaatcagtgtcgttttattatggatcgtctgtataattttagcaataccggtctgcctgacccgaccctgaataccacctatttacaaaccctgcgtggtctgtgtccgctgaatggtaatctgagcgcattggttgattttgatctgcgtaccccgaccatttttgataataaatattatgttaatttggaagaacagaaaggtctgattcagagcgatcaggaactgtttagcagcccgaatgcaaccgataccattccgttggttcgtagctttgcaaatagcacccagaccttttttaatgcatttgttgaagcaatggatcgtatgggcaatattaccccgctgaccggtacccagggtcagattcgtctgaattgtcgtgttgttaatagcaatagcctgctgcatgatatggttgaagttgttgattttgttagcagcatg
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z