BBa_K1297000 1 BBa_K1297000 Klebsiella oxytoca Urease 2014-06-04T11:00:00Z 2015-05-08T01:09:46Z genomic DNA of Klebsiella oxytoca KCTC 1686 This part is complex of pSB1C3 vector and urease gene from Klebsiella oxytoca KCTC 1686. Our main purpose is prevention of desertification by biocementation, and we used this enzyme for biocementation. A function of urease is catalyzing the hydrolysis of urea to release ammonia and carbon dioxide. The released carbon dioxide dissolves in water, and is precipitated with Ca2+, forming CaCO3 (biocement). In our study, we cloned this enzyme with pBAD vector by ligation independent cloning (LIC) for enzyme overexpression, and observed the catalytic activity of it in Escherichia coli by pH indicator assay. To verify microbial biocementation, we performed a biocementation test with synthetic E. coli and detected the whole-cell activity by measuring the radius of precipitated biocement. false false _1660_ 0 8988 9 Not in stock false A size of the urease is very big (about 5 kb) because this gene has many subunits. false Kyoungwoo Jang annotation2379454 1 urease_alpha range2379454 1 1460 3163 annotation2379452 1 urease_gamma range2379452 1 834 1137 annotation2379453 1 urease_beta range2379453 1 1147 1459 annotation2379455 1 UreE range2379455 1 3173 3646 annotation2379456 1 UreF range2379456 1 3637 4321 annotation2379457 1 urease_accessory range2379457 1 4330 4947 annotation2379451 1 UreD range2379451 1 1 825 BBa_K1297000_sequence 1 atgcacggcaccgtgttaccacagaataaaaaaggctggcaggcctcgctggacctccagtttcaatttctcggcgggaaaaccacgctcgcctcccgtcgtcatgtcggtcccctcaccgttcagcgcccgttttatcccgaagaagagacctgccacctctatctgcttcatccgcccggcggtatcgtcggcggcgatgagctcaccattagcgcgacgttggatcccgggtgccacacgctgatcaccatgcccggcgccagcaagttctatcgcagcagcggcgcgcaggcgcgtcttcagcaaaccctgacgctggccgaaaactcaacgctcgagtggctgccgcaggacgccatcttctttcccggcgccaacgccgctttgtctaccgtctttcatctctccgcctccagcacgctgctggcgtgggacctgctgtgccttggccggccggtcatcggcgaagcctttagccacggcgcgctcgccaatcgcctggaggtgtgggtcgacggctctccgctgctgattgagcgcctgagcctggccgatggacagctggcctgcgtcgcgcagcagccgtgggtgggaacgatgctgttctacccggcgaacgaaacgctgctcgaaggcgtacgcgaaaaacttacgccgctggcaaactacgccggcgcgacgctcaccgacggcctgctgacggtacgctttctaagtgacgataatctgctttgccagcgggcgatgcgcgatatctggcaatttatgcgcccgcacctgaccggcaaatctccggtactcccccgaatctggctgacttaagagaacgctatggaactgacacccagagaaaaagacaagctgttgctgtttaccgccgcgctggtggcggagcgtcgcctggcccgcggtctgaagctcaattatcccgaatccgtggccctgattagcgcctttattatggagggcgcgcgcgatggcagaagcgtcgccgagctgatggaagagggacgccacgtcctgagccgcgaccaggtcatggaaggcgtgccggaaatgatccccgatatccaggtcgaagccaccttcccggacggctcgaagctggttaccgtccataacccgataatctgaggtagcgcgatgatcccaggagaatatcaggtaaaacccggccagatagcgattaacgtcggccgcgccacctgctgcattatcgttgaaaatcacggcgaccggccgattcaggtcggctcgcattaccacttcgccgaggtcaacccggcgctgaagttcgatcgccagcaggcgaccggctatcgcctgaatatcgccgccggcaccgcagtgcgcttcgaaccgggccaaaagcgcgaggtggagctggtggcgctggccggaacccgcgcggtgtacggtttccgcggagacgttatgggcgcgctggaggcaaacgatgagtgacatttcacgtcaggcctatgccgacatgttcggccccaccaccggcgataaagttcgcctggcggacaccgagctgtggatcgaggtcgaagatgatttgaccacctacggcgaagaggtcaaattcggcggtggcaaagtgatccgcgacggtatgggccagggacagatgctttccgccgggtgcgtggatctggtgctgaccaatgccctgatcgtcgattactgggggatcgtcaaagccgatatcggcgtcaaagacgggcggatcttcgcgatcggcaaagccggcaacccggatattcagccaaacgtcacgatcgcgatcggcgcggccacggaaattattgcggcggaaggcaagatcgtcaccgccggcggcgtcgatacgcatattcactggatctgtccgcagcaggcggaagaggcgctggtctccggcgtcaccaccatgatcggcggcgggaccggcccggcggcgggcaccaacgccacgacctgcacgcccgggccgtggtacatctcgcggatgctgcaagccgcggacagcctgccggtcaatattggcctgctgggtaaaggcaatggttcgaatccggatgcgctgcgtgagcaggtcgcggccggggttatcggcctgaaaattcacgaagactggggcgcgaccccggcggcaatcgactgcgcgctgacggtggccgacgaaatggacgtacaggtcgctctgcacagcgacaccctcaatgagtccggattcgttgaggataccctggcggccatcggcgggcgcactatccatactttccataccgaaggggcgggcggcggccatgcgccggatattattaccgcctgcgcgcaccccaatattctgccctcgtcgaccaacccgacgctgccctataccgtcaacaccattgacgaacatctggatatgctgatggtttgccatcatctcgacccggatatcgccgaggacgtggcgtttgccgaatcgcgcattcggcgggaaaccattgccgcggaagacgtcctgcacgatctgggcgccttctccctcacctcgtctgattcgcaggcgatgggccgcgtcggcgaagtggtgttgcgcacctggcaggtggcgcaccggatgaaggtccagcgcggcccgctggcggaagagagcggcgataacgacaacttccgtgtgaagcgctatatcgccaaatacaccattaatccggcgttaacccacggtatcgcccatgaagtcggttcgattgaagtggggaaactggcggatctggtgctgtggtccccggcgttcttcggcgtgaagccggcgacgatcgtcaaaggcggcatgatcgcgatggcgccgatgggcgacatcaacgcctctatcccgacgccgcagccggtgcactatcgcccgatgttcggcgcgctgggcagcgctcgccaccgctgccgtttgacctttctgtcgcaggcggcggcggaaaacggcgtcgctgaacggctgaacctgcacagcgcgacggcggtggttaaaggctgccgcacggtacaaaaaggcgatatgcaccacaacggcctgctgcccactattaccgtggattcgcaaacctacgaggtgcgcatcgacggcgaactgataaccagcgaaccggcggacgtgttaccgatggcgcaacgttatttcctgttttaaggagcgcaaatgctttatttgacccaacgggtggagaccccggcgcaggccacggccagcgttaccctgccgattgacgtgcgggtaaaaagccgcattaaagtcacgcttaacgatggccgtcaggcgggcctgctgctgccgcgcggtctgctgctgcgcggcggcgacattctcagtaacgaaaacggtagcgagttcattgaagtgatcgccgctgatgaagccgtttcggtcgtacgctgcgccgatccttttatgctggcgaaggcctgctatcaccttggcaatcgccacgtgccgctacagattatgcccggcgagctgcgctatcatcacgatcacgtcctggacgatatgctgcgccagtttggcctggccgtggatttcgcgcatctgccgtttgaaccggaagccggcgcctacgccagcgaatcgcacggccatcaccaccaccatcacgatcgtgagcacagccactaaatgccgacgccggaaaaacgtctgcgcttgatgcagcttgccagcagcagcctcccggttggcggctacagctggtcccaggggctggagtgggcggtggaagcgggctgggtagcggataccgccgcctttgagcgctggcagctgcgccaaatggagcagagcttttttaccgtcgacctgccgctgtttgcccgcctctaccgggcctgcgaagcgggcgatctggcctgcgcccggcgctggaccgcttatctgctcgcctgccgggaaactcgcgagctgcgcgatgaagagcgcaaccgcggcgcggccttcacccggctgctggccgactggcagccggactgcccgcccgagtggcgcaagctgtgccagcaaagtcagctaaccggcatggcgtggctcggcgtacgctggcaaatcgccattcctgacctggccctgagccttggctatagctggattgaaagcgcggtgatggcgggcgtcaagctggtgccctttggccagcaggcggcccagcagttgattcttcgcctgtgcgaccgctacgcggcggacatggacagcgccctggcgaccccggacgacgccatcggttcggcgacgccgcttgcggctatcgcttccgcccggcacgaaacccagtattcccgattattccgttcttaggagaaggtatgaacatgattaagcaaccgctgcgcgtcggcgtcggcggcccggtcggctccggaaaaaccgcgctgctggaagccctctgcaaagccatgcgcgacacctggcagctggcggtggtgaccaacgatatctacaccaaagaagatcagcgcattctcaccgaggctggcgccctggagcctgaacgcattgtcggcgtcgaaaccggcggctgcccgcataccgctattcgggaagatgcgtcgatgaaccttgccgcggtggaagcgctgagcgagaaattcggcaatctcgatctcatatttgtggaaagcggcggcgataacctgagcgccaccttcagcccggagctggcggatctcaccatctacgtcatcgacgtggcggaaggggaaaagatcccgcgcaaaggcggaccggggatcaccaaatccgacttcctggtgatcaacaaaaccgatcttgcaccgtacgtgggcgcgtctctggaagtgatggagcgcgataccctgcgcatgcgcggcgaacgcccgtggagcttcaccaacctgaaaagcggcgacggcctgcaaaatatcatcgcctttatcgaagacaaaggcatgctcggcaagtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z