LOCUS BBa_K1298003 1243 bp DNA linear 25-Nov-2024 DEFINITION PcChia 1-1 (insert) using J04500 ACCESSION BBa_K1298003 VERSION BBa_K1298003.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 1..220 /label=BBa_J04500 CDS 227..1243 /label=BBa_K1298000 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga agagcatgaa 241 attcagcgct atggctatcg cactgctgac catggcaacg atgaacctgt acttcgtcag 301 cgctgagcag tgcggtcagc aggcgggtgg cgctctgtgc cctggtggtc tgtgttgtag 361 caagtggggt tggtgtggta acaccgatgc acactgcggt caggattgcc agtctcagtg 421 cggtggttct actcctactc caccatctcc aacgccgggt ggtcaaggtg ttgcatctat 481 cattaccgag tccatcttca acgaactgct gaaacaccgt aacgacgcgg gctgcaaagc 541 gtccggcttc tacacctact ccgccttcat cgcagccgct aacgctttcc cgtctttcgg 601 taccaccggc gacgttgcta ctcgtaaacg cgaactggca gcattcttcg gccagacttc 661 ccacgaaacc accggtggct gggcaactgc accggatggt gcatatgctt ggggttattg 721 cttcaaagaa gaacagggca acccgccggc tgaatactgc caggctacta gccagtggcc 781 gtgtgcttct ggtaaacgtt actacggccg tggtccggta caactgagct ggaactacaa 841 ctacggcccg gcgggtaaag cgattggttt cgatggtatc aacaacccgg acatcgtcgc 901 cagcgatgcg actgtgagct ttaaaaccgc gatctggttt tggatgacgg cccagagccc 961 gaaaccgtcc tgccatgacg tgatgaccgg caaatggacc ccgtctggtt ctgattccgc 1021 cgccggtcgt gcggcgggct atggcgcggt taccaatatt attaatggcg gcctggaatg 1081 tggcaaaggc tctgactctc gtcaacagga ccgtatcggc ttttacaaac gctattccga 1141 catcctgggc gtgtcctatg gctccaacct ggactgtaac aatcagcgcc cgtttggcgc 1201 ggcggttcag tctgaagcgc gcctgattaa gaccgttgta tga //