LOCUS       BBa_K1298003            1243 bp    DNA     linear       02-May-2024
DEFINITION  PcChia 1-1 (insert) using J04500
ACCESSION   BBa_K1298003
VERSION     BBa_K1298003.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..1243
                     /label=BBa_K1298000
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga agagcatgaa
      241 attcagcgct atggctatcg cactgctgac catggcaacg atgaacctgt acttcgtcag
      301 cgctgagcag tgcggtcagc aggcgggtgg cgctctgtgc cctggtggtc tgtgttgtag
      361 caagtggggt tggtgtggta acaccgatgc acactgcggt caggattgcc agtctcagtg
      421 cggtggttct actcctactc caccatctcc aacgccgggt ggtcaaggtg ttgcatctat
      481 cattaccgag tccatcttca acgaactgct gaaacaccgt aacgacgcgg gctgcaaagc
      541 gtccggcttc tacacctact ccgccttcat cgcagccgct aacgctttcc cgtctttcgg
      601 taccaccggc gacgttgcta ctcgtaaacg cgaactggca gcattcttcg gccagacttc
      661 ccacgaaacc accggtggct gggcaactgc accggatggt gcatatgctt ggggttattg
      721 cttcaaagaa gaacagggca acccgccggc tgaatactgc caggctacta gccagtggcc
      781 gtgtgcttct ggtaaacgtt actacggccg tggtccggta caactgagct ggaactacaa
      841 ctacggcccg gcgggtaaag cgattggttt cgatggtatc aacaacccgg acatcgtcgc
      901 cagcgatgcg actgtgagct ttaaaaccgc gatctggttt tggatgacgg cccagagccc
      961 gaaaccgtcc tgccatgacg tgatgaccgg caaatggacc ccgtctggtt ctgattccgc
     1021 cgccggtcgt gcggcgggct atggcgcggt taccaatatt attaatggcg gcctggaatg
     1081 tggcaaaggc tctgactctc gtcaacagga ccgtatcggc ttttacaaac gctattccga
     1141 catcctgggc gtgtcctatg gctccaacct ggactgtaac aatcagcgcc cgtttggcgc
     1201 ggcggttcag tctgaagcgc gcctgattaa gaccgttgta tga
//