LOCUS       BBa_K1298004            1243 bp    DNA     linear       27-Dec-2024
DEFINITION  PgeChia 1-1 (insert) using J04500
ACCESSION   BBa_K1298004
VERSION     BBa_K1298004.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..1243
                     /label=BBa_K1298001
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga agtccatccg
      241 tttctccgca atggcgatcg cactggtcac catgggtacc atgaacctgt acttcgctag
      301 cgcagagcag tgcggtcgtc aggcaagcgg tgcactgtgt cctggtggtc tgtgttgtag
      361 caagtggggt tggtgtggta acacggaggc acactgtggt caagactgtc agagccagtg
      421 cggtggttct actcctactc ctccatcccc aactccaggt ggtcaaggtg tcagcagcgt
      481 aatcacggaa agcatcttca acgacctgct gaagcaccgc aacgacgccg catgcaaagc
      541 aaaaggcttc tacagctacg ctgccttcat cgctgccgcc aacgcattcc cggctttcgg
      601 tactaccggc gatctgagca ctcgtaaacg cgaactggct gcgttcttcg gtcaaacctc
      661 ccacgaaacc actggtggtt ggccggctgc tccggatggt ccgtatgctt ggggttattg
      721 ctttaaagaa gaacagggca acccgccggg tgaatactgc caggcttcct cccagtggcc
      781 gtgcgcttct ggtaaacgtt actacggtcg tggtccggta cagatctctt ggaactacaa
      841 ctacggcccg gccggccgtg ccattggctt tgatggcatt aacaacccgg atatcgtagc
      901 gaacgacgcg accgtttctt tcaaaacggc ggtgtggttc tggatgaccg cgcagtctcc
      961 gaaaccgtct tgccatgacg ttatgaccgg ccgttggtct ccgtctggct ctgattctgc
     1021 ggcgggccgt gcggcgggct atggcgttgt taccaatatt atcaatggcg gcctggaatg
     1081 cggcaaaggc tctgattccc gtcaggttga ccgcattggc ttttacaaac gctattgcga
     1141 cattctgggc gtgtcttatg gctccaacct ggactgcaat tcccagaaac cgtttggctt
     1201 tgcggcgcag tcccatccgc gcctgatcaa aaccgtggtg taa
//