BBa_J04500
1
BBa_J04500
IPTG inducible promoter with RBS
2005-06-08T11:00:00Z
2015-08-31T04:08:14Z
Davidson Synth-Aces
Released HQ 2013
R0010.B0034
false
true
_16_
0
326
16
In stock
false
false
Kristen DeCelle
component1508149
1
BBa_R0010
component1508159
1
BBa_B0034
annotation1508159
1
BBa_B0034
range1508159
1
209
220
annotation1508149
1
BBa_R0010
range1508149
1
1
200
BBa_K1298004
1
BBa_K1298004
PgeChia 1-1 (insert) using J04500
2014-06-18T11:00:00Z
2015-05-08T01:09:46Z
BBa_K1298001 (PgeChia 1-1) : Phytochemistry paper of Dr. Kolosova (1), NCBI (protein code) (2), Bio Basic Inc. (DNA optimization)
This part contains an RBS (BBa_B0034), a promoter (lacI inducible; BBa_R0010), a coding region (PgeChia 1-1; BBa_K1298001) and a terminator. It is in a pSB1C3 backbone. With this, the chitinase protein is created. Chitinase breaks down chitin, a substance found in the exoskeleton of arthropods (spiders, insects, etc.) and in the cell walls of fungi. It should be noted that there is no secretory tag, meaning that the chitinase protein is not being secreted outside of the cell, but staying inside. The part should be used to have the chitinase protein break down chitin.
false
false
_1661_
0
19839
9
It's complicated
false
DNA optimization was required to remove illegal cut sites. This was successful. There is also no secretory tag, meaning that the protein (chitinase) is not being transported out of the cell, and instead staying inside.
false
CoBRA Team
component2379266
1
BBa_J04500
component2379267
1
BBa_K1298001
annotation2379266
1
BBa_J04500
range2379266
1
1
220
annotation2379267
1
BBa_K1298001
range2379267
1
227
1243
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1298001
1
BBa_K1298001
PgeChia 1-1
2014-06-09T11:00:00Z
2015-05-08T01:09:46Z
Cloned from Lodgepole pine - check notebook
This is Class I chitinase;
false
false
_1661_
0
19545
9
It's complicated
false
NA
false
CoBRA Team
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961224
1
-35
range1961224
1
137
142
annotation1961227
1
start
range1961227
1
173
173
annotation1961225
1
-10
range1961225
1
161
166
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1298001_sequence
1
atgaagtccatccgtttctccgcaatggcgatcgcactggtcaccatgggtaccatgaacctgtacttcgctagcgcagagcagtgcggtcgtcaggcaagcggtgcactgtgtcctggtggtctgtgttgtagcaagtggggttggtgtggtaacacggaggcacactgtggtcaagactgtcagagccagtgcggtggttctactcctactcctccatccccaactccaggtggtcaaggtgtcagcagcgtaatcacggaaagcatcttcaacgacctgctgaagcaccgcaacgacgccgcatgcaaagcaaaaggcttctacagctacgctgccttcatcgctgccgccaacgcattcccggctttcggtactaccggcgatctgagcactcgtaaacgcgaactggctgcgttcttcggtcaaacctcccacgaaaccactggtggttggccggctgctccggatggtccgtatgcttggggttattgctttaaagaagaacagggcaacccgccgggtgaatactgccaggcttcctcccagtggccgtgcgcttctggtaaacgttactacggtcgtggtccggtacagatctcttggaactacaactacggcccggccggccgtgccattggctttgatggcattaacaacccggatatcgtagcgaacgacgcgaccgtttctttcaaaacggcggtgtggttctggatgaccgcgcagtctccgaaaccgtcttgccatgacgttatgaccggccgttggtctccgtctggctctgattctgcggcgggccgtgcggcgggctatggcgttgttaccaatattatcaatggcggcctggaatgcggcaaaggctctgattcccgtcaggttgaccgcattggcttttacaaacgctattgcgacattctgggcgtgtcttatggctccaacctggactgcaattcccagaaaccgtttggctttgcggcgcagtcccatccgcgcctgatcaaaaccgtggtgtaa
BBa_J04500_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
BBa_K1298004_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaagtccatccgtttctccgcaatggcgatcgcactggtcaccatgggtaccatgaacctgtacttcgctagcgcagagcagtgcggtcgtcaggcaagcggtgcactgtgtcctggtggtctgtgttgtagcaagtggggttggtgtggtaacacggaggcacactgtggtcaagactgtcagagccagtgcggtggttctactcctactcctccatccccaactccaggtggtcaaggtgtcagcagcgtaatcacggaaagcatcttcaacgacctgctgaagcaccgcaacgacgccgcatgcaaagcaaaaggcttctacagctacgctgccttcatcgctgccgccaacgcattcccggctttcggtactaccggcgatctgagcactcgtaaacgcgaactggctgcgttcttcggtcaaacctcccacgaaaccactggtggttggccggctgctccggatggtccgtatgcttggggttattgctttaaagaagaacagggcaacccgccgggtgaatactgccaggcttcctcccagtggccgtgcgcttctggtaaacgttactacggtcgtggtccggtacagatctcttggaactacaactacggcccggccggccgtgccattggctttgatggcattaacaacccggatatcgtagcgaacgacgcgaccgtttctttcaaaacggcggtgtggttctggatgaccgcgcagtctccgaaaccgtcttgccatgacgttatgaccggccgttggtctccgtctggctctgattctgcggcgggccgtgcggcgggctatggcgttgttaccaatattatcaatggcggcctggaatgcggcaaaggctctgattcccgtcaggttgaccgcattggcttttacaaacgctattgcgacattctgggcgtgtcttatggctccaacctggactgcaattcccagaaaccgtttggctttgcggcgcagtcccatccgcgcctgatcaaaaccgtggtgtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z