LOCUS       BBa_K1298005            1252 bp    DNA     linear       22-Dec-2024
DEFINITION  PgeChia 1-2 (insert) using J04500
ACCESSION   BBa_K1298005
VERSION     BBa_K1298005.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..1252
                     /label=BBa_K1298002
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg caggtaacgt
      241 gggtaaaatg tccagcatct tcctgggtac cacgctggct atcttcaccg cagtggctat
      301 gatcatgagc ctgccgtccg tctccagcga ggactgcggt caacaggcag gtggcgcact
      361 gtgtcctggt ggtctgtgtt gtagcaaatg gggttggtgt ggtaacacgc aagctcattg
      421 cggtcaagac tgccagtctc agtgcggtgg cggtggttct actcctactc caactactcc
      481 aaccccaact ccgactactc cgactccgtc tggtcagggt gttgcatcta tcatgacgga
      541 ggacctgttc aaccagctgc tgaagtacaa ggacgactcc cgttgcaagg cgaacggttt
      601 ctacagctac gctgctttca tcgcggcggc atccgcattc tccggtttcg gtaccgcagg
      661 cgatctgacc accaacaaac gtgaactggc tgctttcctg gctcagacca gccacgaaac
      721 caccggtggc tggcagtctg ccccggatgg tccgtatgcc tggggttatt gttttaaaga
      781 agaacaggac ccggtctctg acttctgtca ggctagcagc cagtggccgt gcgcgtctgg
      841 taaacgttac tacggtcgtg gtccgattca gatcagctgg aactacaact acggccaggc
      901 gggctctgcc ctgcaatttg atggcatcaa caacccggat atcgtggcgt ctgatccgac
      961 cgtttctttc aaaacggccg tatggttttg gatgaccgcg cagtccccga aaccgtcctg
     1021 ccacgatgtt atgaccggca cctggtctcc gtctggctct gattctgcgg cgggccgtgc
     1081 cgcgggctat ggcctggtta ccaatattat taatggcggc ctggaatgcg gcaaaggctc
     1141 caacgttaaa caggatgacc gcattggctt ttacaaacgc tactgcgaca tcctgggcgt
     1201 atcctatggc agcaacattg actgcaactc ccagaccccg tatggcggct aa
//