BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961225
1
-10
range1961225
1
161
166
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961224
1
-35
range1961224
1
137
142
annotation1961227
1
start
range1961227
1
173
173
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
BBa_K1298002
1
BBa_K1298002
PgeChia1-2
2014-06-13T11:00:00Z
2015-05-08T01:09:46Z
BioBasic Optimized DNA
PcChia1-1 is a chitinase enyzme cloned from the lodgepole pine (Pinus contorta). Chitinase with The expression of chitinase in combination with one or several different antifungal proteins should have a greater effect on reducing disease development, given the complexities of fungal-plant cell interactions and resistance responses in plants. The effects of plant chitinases on nematode development in vitro and in vivo are worthy of investigation.
Source: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2619419/
false
false
_1661_
0
19545
9
It's complicated
false
Avoiding illegal cutsites.
false
CoBRA Team
BBa_K1298005
1
BBa_K1298005
PgeChia 1-2 (insert) using J04500
2014-06-18T11:00:00Z
2015-05-08T01:09:46Z
BBa_K1298001 (PgeChia 1-1)
*Phytochemistry paper of Dr. Kolosova (1)
*NCBI (protein code) (2)
*Bio Basic Inc. (DNA optimization)
This part contains an RBS (BBa_B0034), a promoter (lacI inducible; BBa_R0010) and a coding region (PgeChia 1-2; BBa_K1298002). It is in a pSB1C3 backbone. With this, the chitinase protein is created. Chitinase breaks down chitin, a substance found in the exoskeleton of arthropods (spiders, insects, etc.) and in the cell walls of fungi. It should be noted that there is no secretory tag, meaning that the chitinase protein is not being secreted outside of the cell, but staying inside. The part should be used to have the chitinase protein break down chitin.
false
false
_1661_
0
19839
9
It's complicated
false
The protein code for the PgeChia 1-2 was optimized to remove illegal cut sites. It should be noted that there is no secretory tag in the biobrick, meaning that the chitinase protein is not being transported outside of the cell, but staying inside of it.
false
CoBRA Team
component2379276
1
BBa_J04500
component2379277
1
BBa_K1298002
annotation2379277
1
BBa_K1298002
range2379277
1
227
1252
annotation2379276
1
BBa_J04500
range2379276
1
1
220
BBa_J04500
1
BBa_J04500
IPTG inducible promoter with RBS
2005-06-08T11:00:00Z
2015-08-31T04:08:14Z
Davidson Synth-Aces
Released HQ 2013
R0010.B0034
false
true
_16_
0
326
16
In stock
false
false
Kristen DeCelle
component1508159
1
BBa_B0034
component1508149
1
BBa_R0010
annotation1508149
1
BBa_R0010
range1508149
1
1
200
annotation1508159
1
BBa_B0034
range1508159
1
209
220
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_B0034_sequence
1
aaagaggagaaa
BBa_J04500_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
BBa_K1298005_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcaggtaacgtgggtaaaatgtccagcatcttcctgggtaccacgctggctatcttcaccgcagtggctatgatcatgagcctgccgtccgtctccagcgaggactgcggtcaacaggcaggtggcgcactgtgtcctggtggtctgtgttgtagcaaatggggttggtgtggtaacacgcaagctcattgcggtcaagactgccagtctcagtgcggtggcggtggttctactcctactccaactactccaaccccaactccgactactccgactccgtctggtcagggtgttgcatctatcatgacggaggacctgttcaaccagctgctgaagtacaaggacgactcccgttgcaaggcgaacggtttctacagctacgctgctttcatcgcggcggcatccgcattctccggtttcggtaccgcaggcgatctgaccaccaacaaacgtgaactggctgctttcctggctcagaccagccacgaaaccaccggtggctggcagtctgccccggatggtccgtatgcctggggttattgttttaaagaagaacaggacccggtctctgacttctgtcaggctagcagccagtggccgtgcgcgtctggtaaacgttactacggtcgtggtccgattcagatcagctggaactacaactacggccaggcgggctctgccctgcaatttgatggcatcaacaacccggatatcgtggcgtctgatccgaccgtttctttcaaaacggccgtatggttttggatgaccgcgcagtccccgaaaccgtcctgccacgatgttatgaccggcacctggtctccgtctggctctgattctgcggcgggccgtgccgcgggctatggcctggttaccaatattattaatggcggcctggaatgcggcaaaggctccaacgttaaacaggatgaccgcattggcttttacaaacgctactgcgacatcctgggcgtatcctatggcagcaacattgactgcaactcccagaccccgtatggcggctaa
BBa_K1298002_sequence
1
atggcaggtaacgtgggtaaaatgtccagcatcttcctgggtaccacgctggctatcttcaccgcagtggctatgatcatgagcctgccgtccgtctccagcgaggactgcggtcaacaggcaggtggcgcactgtgtcctggtggtctgtgttgtagcaaatggggttggtgtggtaacacgcaagctcattgcggtcaagactgccagtctcagtgcggtggcggtggttctactcctactccaactactccaaccccaactccgactactccgactccgtctggtcagggtgttgcatctatcatgacggaggacctgttcaaccagctgctgaagtacaaggacgactcccgttgcaaggcgaacggtttctacagctacgctgctttcatcgcggcggcatccgcattctccggtttcggtaccgcaggcgatctgaccaccaacaaacgtgaactggctgctttcctggctcagaccagccacgaaaccaccggtggctggcagtctgccccggatggtccgtatgcctggggttattgttttaaagaagaacaggacccggtctctgacttctgtcaggctagcagccagtggccgtgcgcgtctggtaaacgttactacggtcgtggtccgattcagatcagctggaactacaactacggccaggcgggctctgccctgcaatttgatggcatcaacaacccggatatcgtggcgtctgatccgaccgtttctttcaaaacggccgtatggttttggatgaccgcgcagtccccgaaaccgtcctgccacgatgttatgaccggcacctggtctccgtctggctctgattctgcggcgggccgtgccgcgggctatggcctggttaccaatattattaatggcggcctggaatgcggcaaaggctccaacgttaaacaggatgaccgcattggcttttacaaacgctactgcgacatcctgggcgtatcctatggcagcaacattgactgcaactcccagaccccgtatggcggctaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z