LOCUS       BBa_K1300001             907 bp    DNA     linear       01-Jun-2024
DEFINITION  pTet promoter + Lock (crRNA) + ccdB + dterm
ACCESSION   BBa_K1300001
VERSION     BBa_K1300001.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..102
                     /label=BBa_J01010
     misc_feature    1..102
                     /label=BBa_J01060
     CDS             440..742
                     /label=ccdB gene coding region
     misc_feature    440..442
                     /label=ccdB gene start codon
     misc_feature    742..744
                     /label=ccdB gene stop codon
     misc_feature    213..213
                     /label=Pt mutation G to A
     misc_feature    431..431
                     /label=Pt mutation A to G
     misc_feature    111..770
                     /label=BBa_K414005
     stem_loop       790..833
                     /label=stem_loop
     misc_feature    779..858
                     /label=BBa_B0010
     terminator      779..858
                     /label=BBa_B0010
     stem_loop       874..893
                     /label=T7 TE
     misc_feature    900..900
                     /label=stop
     polyA_site      894..907
                     /label=polya
     misc_feature    867..907
                     /label=BBa_B0012
     terminator      867..907
                     /label=BBa_B0012
     terminator      779..907
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaactagaa tcacctcttg gatttgggta ttaaagagga gatactagag ggcttactaa
      121 aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg
      181 atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta ttacagtgac
      241 agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg
      301 gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa
      361 aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac
      421 gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta
      481 tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat
      541 ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt
      601 gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc
      661 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
      721 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc tactagagcc
      781 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
      841 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
      901 gtttata
//