##gff-version 3
##sequence-region BBa_K1300001 1 907
BBa_K1300001	.	engineered_region	1	102	.	+	0	ID=BBa_J01060;Name=BBa_J01060
BBa_K1300001	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J01060
BBa_K1300001	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1300001	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1300001	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1300001	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1300001	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1300001	.	mature_transcript_region	63	102	.	+	0	ID=BBa_J01010;Name=BBa_J01010;Parent=BBa_J01060
BBa_K1300001	.	engineered_region	111	770	.	+	0	ID=BBa_K414005;Name=BBa_K414005
BBa_K1300001	.	sequence_alteration	213	213	.	+	0	ID=annotation2102408;Name=Pt mutation G to A;Parent=BBa_K414005
BBa_K1300001	.	sequence_alteration	431	431	.	+	0	ID=annotation2102420;Name=Pt mutation A to G;Parent=BBa_K414005
BBa_K1300001	.	stop_codon	742	744	.	+	0	ID=annotation2086055;Name=ccdB gene stop codon;Parent=BBa_K414005
BBa_K1300001	.	start_codon	440	442	.	+	0	ID=annotation2086054;Name=ccdB gene start codon;Parent=BBa_K414005
BBa_K1300001	.	CDS	440	742	.	+	0	ID=annotation2086053;Name=ccdB gene coding region;Parent=BBa_K414005
BBa_K1300001	.	terminator	779	907	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1300001	.	terminator	779	858	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1300001	.	engineered_region	779	858	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1300001	.	stem_loop	790	833	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1300001	.	terminator	867	907	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1300001	.	stop_codon	900	900	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1300001	.	polyA_site	894	907	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1300001	.	stem_loop	874	893	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1300001	.	engineered_region	867	907	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K1300001
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaactagaatcacctctt
ggatttgggtattaaagaggagatactagagggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttt
tgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgaagcagcgtattacagt
gacagttgacagcgacagctatcagttgctcaaggcatatatgatgtcaatatctccggtctggtaagcacaaccatgc
agaatgaagcccgtcgtctgcgtgccgaacgctggaaagcggaaaatcaggaagggatggctgaggtcgcccggtttat
tgaaatgaacggctcttttgctgacgagaacaggggctggtgaaatgcagtttaaggtttacacctataaaagagagag
ccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagt
gcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatga
ccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaa
aaacgccattaacctgatgttctggggaatataaatgtcaggctcccttatacacagcctactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttata