BBa_K1300001
type: ComponentDefinition
persistent identity: BBa K1300001
display id: BBa_K1300001
version: 1
was generated by: iGEM to SBOL conversion
description: pTet promoter + Lock (crRNA) + ccdB + dterm
created: 2014-06-15T11:00:00Z
modified: 2015-05-08T01:09:46Z
mutable provenance: The components for this part were obtained from the iGEM Registry of Standard Biological Parts.
owned by: james
owned by: myers
mutable description: pTet is a constitutive promoter, meaning that the Cis+ccdB gene is continuously transcribed; however, because the Cis portion of the mRNA forms a hairpin which blocks the RBS, the gene cannot be translated. In this way, the Cis acts as a "lock" which will only allow translation, and therefore cell death, once opened. We designed a separate gene to act as a "key," controlled by a UV-light-inducible promoter (BBa_K1300000).
discontinued: false
dominant: false
experience: experience/None
group u list: _1663_
m user id: 0
owner id: 19613
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We took the pTet+Cis out of the Registry and added a toxin behind it in order to test the riboregulatory system, by means of a kill switch.
star: false
creator: Katty Wu
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J01060
component: BBa_B0015
component: BBa_K414005
sequence annotation: BBa_J01060
sequence annotation: BBa_K414005
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1300001_sequence/1

BBa_J01060
type: Component
persistent identity: component2379214
display id: component2379214
version: 1
top level: BBa_K1300001
definition: [pTET][loc
access: public

BBa_B0015
type: Component
persistent identity: component2379227
display id: component2379227
version: 1
top level: BBa_K1300001
definition: BBa_B0015
access: public

BBa_K414005
type: Component
persistent identity: component2379220
display id: component2379220
version: 1
top level: BBa_K1300001
definition: BBa_K414005
access: public

BBa_J01060
type: SequenceAnnotation
persistent identity: annotation2379214
display id: annotation2379214
version: 1
top level: BBa_K1300001
location: https://synbiohub.org/public/igem/BBa_K1300001/annotation2379214/range2379214/1
component: BBa_J01060

https://synbiohub.org/public/igem/BBa_K1300001/annotation2379214/range2379214/1
type: Range
persistent identity: range2379214
display id: range2379214
version: 1
top level: BBa_K1300001
direction: direction/reverse
start: 1
end: 102
orientation: inline

BBa_K414005
type: SequenceAnnotation
persistent identity: annotation2379220
display id: annotation2379220
version: 1
top level: BBa_K1300001
location: https://synbiohub.org/public/igem/BBa_K1300001/annotation2379220/range2379220/1
component: BBa_K414005

https://synbiohub.org/public/igem/BBa_K1300001/annotation2379220/range2379220/1
type: Range
persistent identity: range2379220
display id: range2379220
version: 1
top level: BBa_K1300001
direction: direction/reverse
start: 111
end: 770
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2379227
display id: annotation2379227
version: 1
top level: BBa_K1300001
location: https://synbiohub.org/public/igem/BBa_K1300001/annotation2379227/range2379227/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1300001/annotation2379227/range2379227/1
type: Range
persistent identity: range2379227
display id: range2379227
version: 1
top level: BBa_K1300001
direction: direction/reverse
start: 779
end: 907
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

[pTET][loc
type: ComponentDefinition
persistent identity: BBa J01060
display id: BBa_J01060
version: 1
was generated by: iGEM to SBOL conversion
description: OnLock1 = [pTet][Lock1]
created: 2005-11-05T12:00:00Z
modified: 2015-08-31T04:08:12Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: [pTet][Lock1]
discontinued: false
dominant: true
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Golden Bear
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J01010
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_J01010
sequence: https://synbiohub.org/public/igem/BBa_J01060_sequence/1

BBa_J01010
type: Component
persistent identity: component1738596
display id: component1738596
version: 1
top level: [pTET][loc
definition: Lock 1
access: public

BBa_R0040
type: Component
persistent identity: component1738591
display id: component1738591
version: 1
top level: [pTET][loc
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1738591
display id: annotation1738591
version: 1
top level: [pTET][loc
location: https://synbiohub.org/public/igem/BBa_J01060/annotation1738591/range1738591/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J01060/annotation1738591/range1738591/1
type: Range
persistent identity: range1738591
display id: range1738591
version: 1
top level: [pTET][loc
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_J01010
type: SequenceAnnotation
persistent identity: annotation1738596
display id: annotation1738596
version: 1
top level: [pTET][loc
location: https://synbiohub.org/public/igem/BBa_J01060/annotation1738596/range1738596/1
component: BBa_J01010

https://synbiohub.org/public/igem/BBa_J01060/annotation1738596/range1738596/1
type: Range
persistent identity: range1738596
display id: range1738596
version: 1
top level: [pTET][loc
direction: direction/forward
start: 63
end: 102
orientation: inline

BBa_K414005
type: ComponentDefinition
persistent identity: BBa K414005
display id: BBa_K414005
version: 1
was generated by: iGEM to SBOL conversion
description: ccdB minimal gene
created: 2010-10-13T11:00:00Z
modified: 2015-06-15T02:56:24Z
mutable provenance: The ccdB gene was obtained from the U-GENT vector pK7FWG2.0
owned by: james
owned by: myers
mutable description: The ccdB gene is a topoisomerase II poison and will kill most cell lines. This is an improvement to the prior ccdB cell death gene (BBa_P1010) in that the size has been reduced and it is now easier to use. CcdB can be used as a selectable marker by mixing it with the desired structure and a plasmid cut with BioBrick restriction enzymes. After ligation and transformation, because ccdB is toxic to most cell lines, the only visible colonies should contain the desired structure in the desired plasmid. CcdB is toxic to most cell lines with the exception of DB3.1 cell lines (BBa_V1005).
discontinued: true
dominant: false
experience: experience/None
group u list: _534_
m user id: 4206
owner id: 7066
owning group id: 9
sample status: It's complicated
status: status/Deleted
bookmark: true
mutable notes: None
star: false
creator: Randy Pares
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Pt mutation G to A
sequence annotation: Pt mutation A to G
sequence annotation: ccdB gene coding region
sequence annotation: ccdB gene start codon
sequence annotation: ccdB gene stop codon
sequence: https://synbiohub.org/public/igem/BBa_K414005_sequence/1

Pt mutation G to A
type: SequenceAnnotation
persistent identity: annotation2102408
display id: annotation2102408
version: 1
top level: BBa_K414005
location: https://synbiohub.org/public/igem/BBa_K414005/annotation2102408/range2102408/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K414005/annotation2102408/range2102408/1
type: Range
persistent identity: range2102408
display id: range2102408
version: 1
top level: BBa_K414005
direction: direction/forward
start: 103
end: 103
orientation: inline

Pt mutation A to G
type: SequenceAnnotation
persistent identity: annotation2102420
display id: annotation2102420
version: 1
top level: BBa_K414005
location: https://synbiohub.org/public/igem/BBa_K414005/annotation2102420/range2102420/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K414005/annotation2102420/range2102420/1
type: Range
persistent identity: range2102420
display id: range2102420
version: 1
top level: BBa_K414005
direction: direction/forward
start: 321
end: 321
orientation: inline

ccdB gene coding region
type: SequenceAnnotation
persistent identity: annotation2086053
display id: annotation2086053
version: 1
top level: BBa_K414005
location: https://synbiohub.org/public/igem/BBa_K414005/annotation2086053/range2086053/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K414005/annotation2086053/range2086053/1
type: Range
persistent identity: range2086053
display id: range2086053
version: 1
top level: BBa_K414005
direction: direction/forward
start: 330
end: 632
orientation: inline

ccdB gene start codon
type: SequenceAnnotation
persistent identity: annotation2086054
display id: annotation2086054
version: 1
top level: BBa_K414005
location: https://synbiohub.org/public/igem/BBa_K414005/annotation2086054/range2086054/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K414005/annotation2086054/range2086054/1
type: Range
persistent identity: range2086054
display id: range2086054
version: 1
top level: BBa_K414005
direction: direction/forward
start: 330
end: 332
orientation: inline

ccdB gene stop codon
type: SequenceAnnotation
persistent identity: annotation2086055
display id: annotation2086055
version: 1
top level: BBa_K414005
location: https://synbiohub.org/public/igem/BBa_K414005/annotation2086055/range2086055/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K414005/annotation2086055/range2086055/1
type: Range
persistent identity: range2086055
display id: range2086055
version: 1
top level: BBa_K414005
direction: direction/forward
start: 632
end: 634
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

Lock 1
type: ComponentDefinition
persistent identity: BBa J01010
display id: BBa_J01010
version: 1
was generated by: iGEM to SBOL conversion
description: Riboregulator Lock 1
created: 2005-11-05T12:00:00Z
modified: 2015-08-31T04:08:12Z
owned by: james
owned by: myers
mutable description: Biobricked version of Isaacs' riboregulator cis repressed lock, crR12.
discontinued: false
dominant: false
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Golden Bear
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence: https://synbiohub.org/public/igem/BBa_J01010_sequence/1

https://synbiohub.org/public/igem/BBa_K1300001_sequence/1
type: Sequence
persistent identity: BBa K1300001 sequence
display id: BBa_K1300001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaactagaatcacctcttggatttgggtattaaagaggagatactagagggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgaagcagcgtattacagtgacagttgacagcgacagctatcagttgctcaaggcatatatgatgtcaatatctccggtctggtaagcacaaccatgcagaatgaagcccgtcgtctgcgtgccgaacgctggaaagcggaaaatcaggaagggatggctgaggtcgcccggtttattgaaatgaacggctcttttgctgacgagaacaggggctggtgaaatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataaatgtcaggctcccttatacacagcctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J01060_sequence/1
type: Sequence
persistent identity: BBa J01060 sequence
display id: BBa_J01060_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaactagaatcacctcttggatttgggtattaaagaggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K414005_sequence/1
type: Sequence
persistent identity: BBa K414005 sequence
display id: BBa_K414005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgaagcagcgtattacagtgacagttgacagcgacagctatcagttgctcaaggcatatatgatgtcaatatctccggtctggtaagcacaaccatgcagaatgaagcccgtcgtctgcgtgccgaacgctggaaagcggaaaatcaggaagggatggctgaggtcgcccggtttattgaaatgaacggctcttttgctgacgagaacaggggctggtgaaatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataaatgtcaggctcccttatacacagcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J01010_sequence/1
type: Sequence
persistent identity: BBa J01010 sequence
display id: BBa_J01010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aactagaatcacctcttggatttgggtattaaagaggaga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z