LOCUS       BBa_K1300004             979 bp    DNA     linear       26-Dec-2024
DEFINITION  Constitutive T7 promoter + Lock (crRNA) + coding GFP
ACCESSION   BBa_K1300004
VERSION     BBa_K1300004.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=T7 promoter
     stem_loop       54..93
                     /label=cis repressed sequence
     promoter        1..93
                     /label=BBa_K1300002
     RBS             102..116
                     /label=RBS-1\Strong
     RBS             109..113
                     /label=RBS
     misc_feature    102..116
                     /label=BBa_B0030
     RBS             102..116
                     /label=BBa_B0030
     CDS             123..842
                     /label=GFP protein
     CDS             123..842
                     /label=BBa_E0040
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
     misc_feature    102..979
                     /label=BBa_E0840
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcacgta ctaaactaga
       61 atcacctctt ggatttgggt attaaagagg agatactaga gattaaagag gagaaatact
      121 agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
      181 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
      241 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
      301 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac
      361 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
      421 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
      481 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
      541 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
      601 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
      661 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
      721 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
      781 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat
      841 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttata
//