LOCUS       BBa_K1300010             377 bp    DNA     linear       26-Nov-2024
DEFINITION  cI repressible promoter + translational ccdB
ACCESSION   BBa_K1300010
VERSION     BBa_K1300010.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     RBS             58..63
                     /label=Shine-Dalgarno RBS
     CDS             72..377
                     /label=ccdB toxin
     misc_feature    58..377
                     /label=BBa_K1300005
     RBS             58..63
                     /label=Shine-Dalgarno RBS
     CDS             72..377
                     /label=ccdB toxin
     misc_feature    58..377
                     /label=BBa_K1300005
     RBS             58..63
                     /label=Shine-Dalgarno RBS
     CDS             72..377
                     /label=ccdB toxin
     misc_feature    58..377
                     /label=BBa_K1300005
     RBS             58..63
                     /label=Shine-Dalgarno RBS
     CDS             72..377
                     /label=ccdB toxin
     misc_feature    58..377
                     /label=BBa_K1300005
     RBS             58..63
                     /label=Shine-Dalgarno RBS
     CDS             72..377
                     /label=ccdB toxin
     misc_feature    58..377
                     /label=BBa_K1300005
     RBS             58..63
                     /label=Shine-Dalgarno RBS
     CDS             72..377
                     /label=ccdB toxin
     misc_feature    58..377
                     /label=BBa_K1300005
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagagg
       61 aggtacagca catgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt
      121 ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg
      181 ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg
      241 gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg
      301 gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga
      361 tgttctgggg aatataa
//