##gff-version 3 ##sequence-region BBa_K1300010 1 377 BBa_K1300010 . promoter 1 49 . + 0 ID=BBa_R0051;Name=BBa_R0051 BBa_K1300010 . engineered_region 1 49 . + 0 ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051 BBa_K1300010 . promoter 38 43 . + 0 ID=annotation2022;Name=-10;Parent=BBa_R0051 BBa_K1300010 . non_covalent_binding_site 25 41 . + 0 ID=annotation2024;Name=OR1;Parent=BBa_R0051 BBa_K1300010 . promoter 15 20 . + 0 ID=annotation2023;Name=-35;Parent=BBa_R0051 BBa_K1300010 . non_covalent_binding_site 1 17 . + 0 ID=annotation2025;Name=OR2;Parent=BBa_R0051 BBa_K1300010 . engineered_region 58 377 . + 0 ID=BBa_K1300005;Name=BBa_K1300005 BBa_K1300010 . ribosome_entry_site 58 63 . + 0 ID=annotation2379237;Name=Shine-Dalgarno RBS;Parent=BBa_K1300005 BBa_K1300010 . CDS 72 377 . + 0 ID=annotation2379238;Name=ccdB toxin;Parent=BBa_K1300005 BBa_K1300010 . promoter 1 49 . + 0 ID=BBa_R0051;Name=BBa_R0051 BBa_K1300010 . engineered_region 1 49 . + 0 ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051 BBa_K1300010 . promoter 38 43 . + 0 ID=annotation2022;Name=-10;Parent=BBa_R0051 BBa_K1300010 . non_covalent_binding_site 25 41 . + 0 ID=annotation2024;Name=OR1;Parent=BBa_R0051 BBa_K1300010 . promoter 15 20 . + 0 ID=annotation2023;Name=-35;Parent=BBa_R0051 BBa_K1300010 . non_covalent_binding_site 1 17 . + 0 ID=annotation2025;Name=OR2;Parent=BBa_R0051 BBa_K1300010 . engineered_region 58 377 . + 0 ID=BBa_K1300005;Name=BBa_K1300005 BBa_K1300010 . ribosome_entry_site 58 63 . + 0 ID=annotation2379237;Name=Shine-Dalgarno RBS;Parent=BBa_K1300005 BBa_K1300010 . CDS 72 377 . + 0 ID=annotation2379238;Name=ccdB toxin;Parent=BBa_K1300005 BBa_K1300010 . engineered_region 58 377 . + 0 ID=BBa_K1300005;Name=BBa_K1300005 BBa_K1300010 . ribosome_entry_site 58 63 . + 0 ID=annotation2379237;Name=Shine-Dalgarno RBS;Parent=BBa_K1300005 BBa_K1300010 . CDS 72 377 . + 0 ID=annotation2379238;Name=ccdB toxin;Parent=BBa_K1300005 BBa_K1300010 . promoter 1 49 . + 0 ID=BBa_R0051;Name=BBa_R0051 BBa_K1300010 . engineered_region 1 49 . + 0 ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051 BBa_K1300010 . promoter 38 43 . + 0 ID=annotation2022;Name=-10;Parent=BBa_R0051 BBa_K1300010 . non_covalent_binding_site 25 41 . + 0 ID=annotation2024;Name=OR1;Parent=BBa_R0051 BBa_K1300010 . promoter 15 20 . + 0 ID=annotation2023;Name=-35;Parent=BBa_R0051 BBa_K1300010 . non_covalent_binding_site 1 17 . + 0 ID=annotation2025;Name=OR2;Parent=BBa_R0051 BBa_K1300010 . engineered_region 58 377 . + 0 ID=BBa_K1300005;Name=BBa_K1300005 BBa_K1300010 . ribosome_entry_site 58 63 . + 0 ID=annotation2379237;Name=Shine-Dalgarno RBS;Parent=BBa_K1300005 BBa_K1300010 . CDS 72 377 . + 0 ID=annotation2379238;Name=ccdB toxin;Parent=BBa_K1300005 BBa_K1300010 . promoter 1 49 . + 0 ID=BBa_R0051;Name=BBa_R0051 BBa_K1300010 . engineered_region 1 49 . + 0 ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051 BBa_K1300010 . promoter 38 43 . + 0 ID=annotation2022;Name=-10;Parent=BBa_R0051 BBa_K1300010 . non_covalent_binding_site 25 41 . + 0 ID=annotation2024;Name=OR1;Parent=BBa_R0051 BBa_K1300010 . promoter 15 20 . + 0 ID=annotation2023;Name=-35;Parent=BBa_R0051 BBa_K1300010 . non_covalent_binding_site 1 17 . + 0 ID=annotation2025;Name=OR2;Parent=BBa_R0051 BBa_K1300010 . engineered_region 58 377 . + 0 ID=BBa_K1300005;Name=BBa_K1300005 BBa_K1300010 . ribosome_entry_site 58 63 . + 0 ID=annotation2379237;Name=Shine-Dalgarno RBS;Parent=BBa_K1300005 BBa_K1300010 . CDS 72 377 . + 0 ID=annotation2379238;Name=ccdB toxin;Parent=BBa_K1300005 BBa_K1300010 . promoter 1 49 . + 0 ID=BBa_R0051;Name=BBa_R0051 BBa_K1300010 . engineered_region 1 49 . + 0 ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051 BBa_K1300010 . promoter 38 43 . + 0 ID=annotation2022;Name=-10;Parent=BBa_R0051 BBa_K1300010 . non_covalent_binding_site 25 41 . + 0 ID=annotation2024;Name=OR1;Parent=BBa_R0051 BBa_K1300010 . promoter 15 20 . + 0 ID=annotation2023;Name=-35;Parent=BBa_R0051 BBa_K1300010 . non_covalent_binding_site 1 17 . + 0 ID=annotation2025;Name=OR2;Parent=BBa_R0051 BBa_K1300010 . promoter 1 49 . + 0 ID=BBa_R0051;Name=BBa_R0051 BBa_K1300010 . engineered_region 1 49 . + 0 ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051 BBa_K1300010 . promoter 38 43 . + 0 ID=annotation2022;Name=-10;Parent=BBa_R0051 BBa_K1300010 . non_covalent_binding_site 25 41 . + 0 ID=annotation2024;Name=OR1;Parent=BBa_R0051 BBa_K1300010 . promoter 15 20 . + 0 ID=annotation2023;Name=-35;Parent=BBa_R0051 BBa_K1300010 . non_covalent_binding_site 1 17 . + 0 ID=annotation2025;Name=OR2;Parent=BBa_R0051 BBa_K1300010 . engineered_region 58 377 . + 0 ID=BBa_K1300005;Name=BBa_K1300005 BBa_K1300010 . ribosome_entry_site 58 63 . + 0 ID=annotation2379237;Name=Shine-Dalgarno RBS;Parent=BBa_K1300005 BBa_K1300010 . CDS 72 377 . + 0 ID=annotation2379238;Name=ccdB toxin;Parent=BBa_K1300005 >BBa_K1300010 taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaggaggtacagcacatgcagtt taaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccggg cgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcata tcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctga tctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataa