LOCUS       BBa_K1300011            1871 bp    DNA     linear       22-Nov-2024
DEFINITION  cI repressible promoter + GFP + pBAD + cI repressor
ACCESSION   BBa_K1300011
VERSION     BBa_K1300011.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..795
                     /label=GFP protein
     CDS             76..795
                     /label=BBa_E0040
     stem_loop       815..858
                     /label=stem_loop
     misc_feature    804..883
                     /label=BBa_B0010
     terminator      804..883
                     /label=BBa_B0010
     stem_loop       899..918
                     /label=T7 TE
     misc_feature    925..925
                     /label=stop
     polyA_site      919..932
                     /label=polya
     misc_feature    892..932
                     /label=BBa_B0012
     terminator      892..932
                     /label=BBa_B0012
     terminator      804..932
                     /label=BBa_B0015
     promoter        941..1070
                     /label=BBa_I13453
     misc_feature    1083..1086
                     /label=conserved
     RBS             1079..1090
                     /label=BBa_B0034
     CDS             1100..1807
                     /label=cI lambda
     misc_feature    1808..1840
                     /label=LVA
     misc_feature    1847..1871
                     /label=Help:Barcodes
     CDS             1097..1871
                     /label=BBa_C0051
     misc_feature    58..1871
                     /label=BBa_K611014
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt
      121 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
      181 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
      241 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca
      301 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      361 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
      421 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
      481 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
      541 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
      601 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
      661 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
      721 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
      781 ctatacaaat aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      841 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
      901 ctcaccttcg ggtgggcctt tctgcgttta tatactagag acattgatta tttgcacggc
      961 gtcacacttt gctatgccat agcattttta tccataagat tagcggatcc tacctgacgc
     1021 tttttatcgc aactctctac tgtttctcca taccgttttt ttgggctagc tactagagaa
     1081 agaggagaaa tactagatga gcacaaaaaa gaaaccatta acacaagagc agcttgagga
     1141 cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat gaacttggct tatcccagga
     1201 atctgtcgca gacaagatgg ggatggggca gtcaggcgtt ggtgctttat ttaatggcat
     1261 caatgcatta aatgcttata acgccgcatt gcttgcaaaa attctcaaag ttagcgttga
     1321 agaatttagc ccttcaatcg ccagagaaat ctacgagatg tatgaagcgg ttagtatgca
     1381 gccgtcactt agaagtgagt atgagtaccc tgttttttct catgttcagg cagggatgtt
     1441 ctcacctgag cttagaacct ttaccaaagg tgatgcggag agatgggtaa gcacaaccaa
     1501 aaaagccagt gattctgcat tctggcttga ggttgaaggt aattccatga ccgcaccaac
     1561 aggctccaag ccaagctttc ctgacggaat gttaattctc gttgaccctg agcaggctgt
     1621 tgagccaggt gatttctgca tagccagact tgggggtgat gagtttacct tcaagaaact
     1681 gatcagggat agcggtcagg tgtttttaca accactaaac ccacagtacc caatgatccc
     1741 atgcaatgag agttgttccg ttgtggggaa agttatcgct agtcagtggc ctgaagagac
     1801 gtttggcgct gcaaacgacg aaaactacgc tttagtagct taataacgct gatagtgcta
     1861 gtgtagatcg c
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