LOCUS BBa_K131010 4144 bp DNA linear 25-Nov-2024 DEFINITION AHL Inducible Colicin E2 with GFP ACCESSION BBa_K131010 VERSION BBa_K131010.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 terminator 870..998 /label=BBa_B0015 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1..1061 /label=BBa_F2620 misc_feature 1074..1077 /label=conserved RBS 1070..1081 /label=BBa_B0034 CDS 1088..1807 /label=GFP protein CDS 1088..1807 /label=BBa_E0040 misc_feature 1070..1807 /label=BBa_I13500 CDS 1839..3584 /label=CeaB CDS 3587..3847 /label=CeiB CDS 3909..4052 /label=CelB misc_feature 1816..4144 /label=BBa_K131009 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa 1081 atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt 1141 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac 1201 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc 1261 aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat 1321 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat 1381 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac 1441 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg 1501 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa 1561 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact 1621 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa 1681 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat 1741 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa 1801 ataataatac tagagaacgc ttaaaagagg gaatttttat gagcggtggc gatggacgcg 1861 gccataacac gggcgcgcat agcacaagtg gtaacattaa tggtggcccg accgggcttg 1921 gtgtaggtgg tggtgcttct gatggctccg gatggagttc ggaaaataac ccgtggggtg 1981 gtggttccgg tagcggcatt cactggggtg gtggttccgg tcatggtaat ggcgggggga 2041 atggtaattc cggtggtggt tcgggaacag gcggtaatct gtcagcagta gctgcgccag 2101 tggcatttgg ttttccggca ctttccactc caggagctgg cggtctggcg gtcagtattt 2161 cagcgggagc attatcggca gctattgctg atattatggc tgccctgaaa ggaccgttta 2221 aatttggtct ttggggggtg gctttatatg gtgtattgcc atcacaaata gcgaaagatg 2281 accccaatat gatgtcaaag attgtgacgt cattacccgc agatgatatt actgaatcac 2341 ctgtcagttc attacctctc gataaggcaa cagtaaacgt aaatgttcgt gttgttgatg 2401 atgtaaaaga cgaacgacag aatatttcgg ttgtttcagg tgttccgatg agtgttccgg 2461 tggttgatgc aaaacctacc gaacgtccag gtgtttttac ggcatcaatt ccaggtgcac 2521 ctgttctgaa tatttcagtt aataacagta cgccagaagt acagacatta agcccaggtg 2581 ttacaaataa tactgataag gatgttcgcc cggcaggatt tactcagggt ggtaatacca 2641 gggatgcagt tattcgattc ccgaaggaca gcggtcataa tgccgtatat gtttcagtga 2701 gtgatgttct tagtcctgac caggtaaaac aacgtcagga tgaagaaaat cgccgtcagc 2761 aggaatggga tgctacgcat ccggttgaag cggctgagcg aaattatgaa cgcgcgcgtg 2821 cagagctgaa tcaggcaaat gaagatgttg ccagaaatca ggagcgacag gctaaagctg 2881 ttcaggttta taattcgcgt aaaagcgaac ttgatgcagc gaataaaact cttgctgatg 2941 caatagctga aataaaacaa tttaatcgat ttgcccatga cccaatggct ggcggtcaca 3001 gaatgtggca aatggccgga cttaaagctc agcgggcgca gacggatgta aataataagc 3061 aggctgcatt tgatgctgct gcaaaagaga agtcagatgc tgatgctgca ttaagtgccg 3121 cgcaggagcg ccgcaaacag aaggaaaata aagaaaagga cgctaaggat aaattagata 3181 aggagagtaa acggaataag ccagggaagg cgacaggtaa aggtaaacca gttggtgata 3241 aatggctgga tgatgcaggt aaagattcag gagcgccaat tccagatcgc attgctgata 3301 agttgcgtga taaagaattt aaaaactttg acgatttccg gaagaaattc tgggaagaag 3361 tgtcaaaaga tcccgatctt agtaagcaat ttaaaggcag taataagacg aacattcaaa 3421 agggaaaagc accttttgca aggaagaaag accaagtagg tggtagggaa cgctttgaat 3481 tacatcatga taaaccaatc agtcaggatg gtggtgtcta tgatatgaat aatatcagag 3541 tgaccacacc taagcgacat attgatattc atcggggtaa gtaaaaatgg aactgaaaca 3601 tagtattagt gattataccg aggctgaatt tctggagttt gtaaaaaaaa tatgtagagc 3661 tgaaggtgct actgaagagg atgacaataa attagtgaga gagtttgagc gattaactga 3721 gcacccagat ggttcagatc tgatttatta tcctcgcgat gacagggaag atagtcctga 3781 agggattgtc aaggaaatta aagaatggcg agctgctaac ggtaagtcag gatttaaaca 3841 gggctgaaat atgaatgccg gttgtttatg gatgaatggc tggcattctt tcacaacaag 3901 gagtcgttat gaaaaaaata acagggatta ttttattgct tcttgcagtc attattctgt 3961 ctgcatgtca ggcaaactat atccgggatg ttcagggcgg gaccgtatct ccgtcatcaa 4021 cagctgaagt gaccggatta gcaacgcagt aacccgaaat cctctttgac aaaaacaaag 4081 cgtgtcaggc tgattctgat gcgctttttt ttgaaatgtc acaaaaattc catgtgggag 4141 atgg //