##gff-version 3
##sequence-region BBa_K131015 30 3706
BBa_K131015	.	point_mutation	599	599	.	+	0	ID=annotation1976852;Name=Silent mutation to remove EcoRI site
BBa_K131015	.	start_codon	30	32	.	+	0	ID=annotation1976844;Name=Start of LuxP
BBa_K131015	.	CDS	30	1127	.	+	0	ID=annotation1976850;Name=LuxP
BBa_K131015	.	stop_codon	3704	3706	.	+	0	ID=annotation1976847;Name=Stop for LuxQ
BBa_K131015	.	stop_codon	1125	1127	.	+	0	ID=annotation1976845;Name=Stop for LuxP
BBa_K131015	.	point_mutation	1465	1465	.	+	0	ID=annotation1976853;Name=Silent mutation to remove EcoRI site
BBa_K131015	.	point_mutation	1618	1618	.	+	0	ID=annotation1976854;Name=Silent mutation to remove XbaI site
BBa_K131015	.	CDS	1127	3706	.	+	0	ID=annotation1976851;Name=LuxQ
BBa_K131015	.	start_codon	1127	1129	.	+	0	ID=annotation1976846;Name=Start of LuxQ
>BBa_K131015
atgctcgataaaaactaaaagagcaatagatgaagaaagcgttactattttccctgatttctatggtcggtttttctcc
agcgtctcaagcaacacaagttttgaatgggtactggggttatcaagagtttttggacgagtttcccgagcaacgaaat
ctgaccaatgctttatcagaagcagtacgagcacagccggtcccactgtctaaaccgacacaacgcccgattaaaatat
cagtggtttacccaggacagcaagtttcagattactgggtaagaaatattgcatcattcgaaaaacgtttgtataagtt
gaatattaactaccaactgaaccaagtgtttactcgtccaaatgctgatatcaagcaacaaagcttgtcattaatggaa
gcgctcaagagcaaatcggattacttgattttcacgcttgatacgacaagacaccgtaaatttgttgagcacgttttgg
actcaacgaacaccaaattgatcttgcaaaatatcactacaccagtccgtgagtgggacaaacatcaaccgtttttata
tgtcggatttgaccacgcagaaggcagtcgtgaattagcaacagagttcggaaagttcttcccaaaacacacatattac
agtgtgctctacttttctgaaggttatattagcgatgtgagaggtgatacttttattcaccaagtaaaccgtgataata
actttgagctacaatcagcgtattacacgaaggcaaccaagcaatccggctatgatgctgcgaaagcgagtttagcaaa
acatccagatgttgattttatctatgcatgttcgaccgacgtagcattaggtgcagtagacgcactggctgagttggga
cgtgaagatattatgatcaatggctggggtggaggctctgctgagttagacgctatccagaagggtgatttagacatca
ccgtcatgcgtatgaatgatgacactggcatagccatggcagaagcgattaagtgggacttggaagataaaccagttcc
gaccgtatactcaggtgactttgaaatcgtaacaaaggcagattcaccggagagaatcgaagcgctgaaaaagcgcgca
tttagatattcagataattgatgacaacaacgcgatcaaacattaaaaagcgtcgctcgctggcgacgctcataacaaa
gatcatcattttagttcttgccccaattattctggggattttcattcagagctattacttctccaagcaaatcatttgg
caagaagtagaccgaaccaaacagcaaacctctgcactgatccacaatatatttgatagccactttgcggcgatccaga
tacatcatgacagtaattccaagagcgaagtcattcgtgatttctacactgatcgcgacacggatgtgctcaacttttt
cttcctcagtatcgaccaaagcgatccgtcgcacacaccagagttccgttttctaacggaccacaaaggcatcatttgg
gacgatggaaatgcgcatttctatggtgtgaacgaccttatccttgatagccttgccaatcgggtcagtttcagtaaca
actggtattacattaatgtcatgacctccattggttcgagacacatgctcgtgcgccgtgtgccgatcctagacccttc
aacaggagaggtgcttggtttctcatttaatgccgtcgtcttagacaacaacttcgctttgatggaaaagctcaagagt
gaaagtaacgtcgacaatgtggtgctggttgctaatagcgttcctttagcaaactctttgattggtgatgagccatata
acgttgctgatgtattgcagcgtaaaagttcagacaaaagactcgataagctgttggtaatagaaacgccaatcgtcgt
aaatgcagtgactaccgagctttgcttgttgacggtacaagacaatcagagtgtggtgacattacaaatccaacatatt
ctagccatgcttgcatcgatcatcggtatgatcatgattgccttaatgagtagggaatggattgagagtaaagtttcgg
cgcagttagaatctttgatgtcttacacccgctctgctcgtgaggaaaaagggtttgaacgatttggcggttcggatat
tgaagagtttgatcacatcggttcaacccttgaaagtacattcgaagagcttgaagcgcagaagaagtcgttccgagat
ctgtttaattttgccttatcacccatcatggtttggtctgaagagagtgtcctgattcagatgaaccctgccgcgcgca
aagaattagtgatcgaagacgatcatgaaatcatgcatccggtcttccaaggctttaaagagaaattgaccccacacct
caaaatggcggctcaaggtgcgacgttgactggggtgaacgtgcctattggtaataagatctaccgatggaacttgtcg
ccaattcgtgttgatggcgatatcagtggcattattgtgcaaggccaagacattacaacacttatcgaagccgagaagc
agagtaacattgcgcgtagagaagcagaaaaatcggcgcaagcacgtgcagacttccttgctaaaatgagccatgaaat
tcgtacgccaatcaacggcattttaggtgtcgcccaattattgaaagattctgtcgatacacaagagcagaagaatcaa
atcgacgtcctgtgccacagtggcgagcacttgcttgcagtactgaacgatattctcgatttctcaaagatagagcagg
gcaagttcaatattcagaaacacccgttctccttcaccgataccatgcgtacattggaaaatatttatcgtccgatttg
cacaaataagggggtggagttggtcatcgagaatgagcttgacccgaatgttgaaatcttcaccgatcaagtccgcttg
aatcagattctatttaacttagtgagtaatgccgttaagttcacgccgattggctcgattcgactgcacgcagaacttg
aacaattctatggtgcggagaacagcgtgttagttgtggaactgactgatactggcatcggcattgaaagcgataagct
cgaccaaatgttcgaaccttttgtgcaagaagagtcgacaaccacacgcgaatatggcggtagcggcctaggtttgacc
atcgttaagaacctagtcgatatgttagaaggtgatgttcaggtccgcagtagcaaggggggggggacaacatttgtta
taacacttccagtaaaagatcgtgagcgtgtcttaaggcctctggaggtcagtcaacgtatcaagccggaagccttgtt
tgatgaaagtttaaaagtgctactggtggaagataaccataccaatgcgtttatccttcaggctttctgtaagaagtat
aaaatgcaggtggattgggcgaaagatgggctggacgcgatggagctcctttctgataccacctacgatctgatcctca
tggataaccaattaccccaccttggtggtattgagaccacgcacgagattcgccagaacttgaggcttggaacgccaat
ttacgcgtgtacagcagacaccgcgaaagaaaccagtgatgcgtttatggcggcaggtgcaaactatgtcatgctgaag
ccaattaaagagaatgcgttacatgaggcgtttgtcgatttcaaacaacgtttcttggtagaaagaacctaacggttta
atggcagtgataagttaggggctgaagttaaataataaaagtaaagaagggagcgtaatgcactaatgccgttcactta
aggtgatcggcattgttttttctagggtatcgg