LOCUS BBa_K131018 1719 bp DNA linear 22-Nov-2024 DEFINITION Intermediate for the Response circuit ACCESSION BBa_K131018 VERSION BBa_K131018.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 22..729 /label=cI lambda misc_feature 730..762 /label=LVA misc_feature 769..793 /label=Help:Barcodes CDS 19..768 /label=BBa_C0051 stem_loop 813..856 /label=stem_loop misc_feature 802..881 /label=BBa_B0010 terminator 802..881 /label=BBa_B0010 stem_loop 897..916 /label=T7 TE misc_feature 923..923 /label=stop polyA_site 917..930 /label=polya misc_feature 890..930 /label=BBa_B0012 terminator 890..930 /label=BBa_B0012 promoter 976..981 /label=-10 promoter 953..958 /label=-35 misc_feature 963..979 /label=OR1 misc_feature 939..955 /label=OR2 misc_feature 939..987 /label=BBa_R0051 promoter 939..987 /label=BBa_R0051 misc_feature 1..987 /label=BBa_Q04510 misc_feature 1000..1003 /label=conserved RBS 996..1007 /label=BBa_B0034 CDS 1014..1688 /label=mrfp1 misc_feature 1695..1719 /label=Help:Barcodes CDS 1014..1694 /label=BBa_E1010 misc_feature 996..1719 /label=BBa_I13502 ORIGIN 1 aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag 61 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag 121 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc 181 atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt 241 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg 301 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg 361 ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc 421 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca 481 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct 541 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa 601 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc 661 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag 721 acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc 781 tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 901 caccttcggg tgggcctttc tgcgtttata tactagagta acaccgtgcg tgttgactat 961 tttacctctg gcggtgataa tggttgctac tagagaaaga ggagaaatac tagatggctt 1021 cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta 1081 acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga 1141 ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc 1201 cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc 1261 tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg 1321 gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta 1381 aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa accatgggtt 1441 gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa 1501 tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg 1561 ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct 1621 cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca 1681 ccggtgctta ataacgctga tagtgctagt gtagatcgc //