LOCUS       BBa_K131019             2002 bp    DNA     linear       18-Feb-2025
DEFINITION  LuxO inducible RFP
ACCESSION   BBa_K131019
VERSION     BBa_K131019.1  
FEATURES             Location/Qualifiers
     misc_feature    250..254
                     /label=-24 region
     misc_feature    262..264
                     /label=-12 region
     promoter        1..275
                     /label=BBa_K131017
     misc_feature    288..291
                     /label=conserved
     RBS             284..295
                     /label=BBa_B0034
     CDS             305..1012
                     /label=cI lambda
     misc_feature    1013..1045
                     /label=LVA
     misc_feature    1052..1076
                     /label=Help:Barcodes
     CDS             302..1051
                     /label=BBa_C0051
     stem_loop       1096..1139
                     /label=stem_loop
     misc_feature    1085..1164
                     /label=BBa_B0010
     terminator      1085..1164
                     /label=BBa_B0010
     stem_loop       1180..1199
                     /label=T7 TE
     misc_feature    1206..1206
                     /label=stop
     polyA_site      1200..1213
                     /label=polya
     misc_feature    1173..1213
                     /label=BBa_B0012
     terminator      1173..1213
                     /label=BBa_B0012
     promoter        1259..1264
                     /label=-10
     promoter        1236..1241
                     /label=-35
     misc_feature    1246..1262
                     /label=OR1
     misc_feature    1222..1238
                     /label=OR2
     misc_feature    1222..1270
                     /label=BBa_R0051
     promoter        1222..1270
                     /label=BBa_R0051
     misc_feature    284..1270
                     /label=BBa_Q04510
     misc_feature    1283..1286
                     /label=conserved
     RBS             1279..1290
                     /label=BBa_B0034
     CDS             1297..1971
                     /label=mrfp1
     misc_feature    1978..2002
                     /label=Help:Barcodes
     CDS             1297..1977
                     /label=BBa_E1010
     misc_feature    1279..2002
                     /label=BBa_I13502
ORIGIN
        1 gtatcagcaa aaacactacg gtggataatc agtaaaacca tgaaactaga gccccgcaca
       61 cttgcggggc tttttaattt tgaatttctt tcttattaaa acgccatttt tctgataaat
      121 gtattagtag caatgcgcat ggtggcatat ttgcatcatt ttgcattttg caaatgcgat
      181 ttgcaaaatg cgtgctcaat aaagcaccaa tatgcatcag gatcgaagaa aaaaggcgtt
      241 tttaaaagtt ggcacgcatc gtgctttata cagattacta gagaaagagg agaaatacta
      301 gatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac gtcgccttaa
      361 agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg tcgcagacaa
      421 gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg cattaaatgc
      481 ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat ttagcccttc
      541 aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt cacttagaag
      601 tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac ctgagcttag
      661 aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag ccagtgattc
      721 tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag
      781 ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt
      841 ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg
      901 tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg
      961 ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gcgctgcaaa
     1021 cgacgaaaac tacgctttag tagcttaata acgctgatag tgctagtgta gatcgctact
     1081 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1141 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1201 ttctgcgttt atatactaga gtaacaccgt gcgtgttgac tattttacct ctggcggtga
     1261 taatggttgc tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc
     1321 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa
     1381 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt
     1441 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt
     1501 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg
     1561 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc
     1621 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac
     1681 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa
     1741 cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa
     1801 gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag
     1861 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac
     1921 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc
     1981 tgatagtgct agtgtagatc gc
//