##gff-version 3
##sequence-region BBa_K131020 1 3887
BBa_K131020	.	engineered_region	1	3825	.	+	0	ID=BBa_K131015;Name=BBa_K131015
BBa_K131020	.	start_codon	30	32	.	+	0	ID=annotation1976844;Name=Start of LuxP;Parent=BBa_K131015
BBa_K131020	.	CDS	30	1127	.	+	0	ID=annotation1976850;Name=LuxP;Parent=BBa_K131015
BBa_K131020	.	stop_codon	3704	3706	.	+	0	ID=annotation1976847;Name=Stop for LuxQ;Parent=BBa_K131015
BBa_K131020	.	point_mutation	1465	1465	.	+	0	ID=annotation1976853;Name=Silent mutation to remove EcoRI site;Parent=BBa_K131015
BBa_K131020	.	point_mutation	599	599	.	+	0	ID=annotation1976852;Name=Silent mutation to remove EcoRI site;Parent=BBa_K131015
BBa_K131020	.	start_codon	1127	1129	.	+	0	ID=annotation1976846;Name=Start of LuxQ;Parent=BBa_K131015
BBa_K131020	.	CDS	1127	3706	.	+	0	ID=annotation1976851;Name=LuxQ;Parent=BBa_K131015
BBa_K131020	.	point_mutation	1618	1618	.	+	0	ID=annotation1976854;Name=Silent mutation to remove XbaI site;Parent=BBa_K131015
BBa_K131020	.	stop_codon	1125	1127	.	+	0	ID=annotation1976845;Name=Stop for LuxP;Parent=BBa_K131015
BBa_K131020	.	promoter	3834	3887	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K131020	.	promoter	3876	3881	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K131020	.	non_covalent_binding_site	3859	3877	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K131020	.	non_covalent_binding_site	3834	3852	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K131020	.	engineered_region	3834	3887	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K131020	.	promoter	3853	3858	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
>BBa_K131020
atgctcgataaaaactaaaagagcaatagatgaagaaagcgttactattttccctgatttctatggtcggtttttctcc
agcgtctcaagcaacacaagttttgaatgggtactggggttatcaagagtttttggacgagtttcccgagcaacgaaat
ctgaccaatgctttatcagaagcagtacgagcacagccggtcccactgtctaaaccgacacaacgcccgattaaaatat
cagtggtttacccaggacagcaagtttcagattactgggtaagaaatattgcatcattcgaaaaacgtttgtataagtt
gaatattaactaccaactgaaccaagtgtttactcgtccaaatgctgatatcaagcaacaaagcttgtcattaatggaa
gcgctcaagagcaaatcggattacttgattttcacgcttgatacgacaagacaccgtaaatttgttgagcacgttttgg
actcaacgaacaccaaattgatcttgcaaaatatcactacaccagtccgtgagtgggacaaacatcaaccgtttttata
tgtcggatttgaccacgcagaaggcagtcgtgaattagcaacagagttcggaaagttcttcccaaaacacacatattac
agtgtgctctacttttctgaaggttatattagcgatgtgagaggtgatacttttattcaccaagtaaaccgtgataata
actttgagctacaatcagcgtattacacgaaggcaaccaagcaatccggctatgatgctgcgaaagcgagtttagcaaa
acatccagatgttgattttatctatgcatgttcgaccgacgtagcattaggtgcagtagacgcactggctgagttggga
cgtgaagatattatgatcaatggctggggtggaggctctgctgagttagacgctatccagaagggtgatttagacatca
ccgtcatgcgtatgaatgatgacactggcatagccatggcagaagcgattaagtgggacttggaagataaaccagttcc
gaccgtatactcaggtgactttgaaatcgtaacaaaggcagattcaccggagagaatcgaagcgctgaaaaagcgcgca
tttagatattcagataattgatgacaacaacgcgatcaaacattaaaaagcgtcgctcgctggcgacgctcataacaaa
gatcatcattttagttcttgccccaattattctggggattttcattcagagctattacttctccaagcaaatcatttgg
caagaagtagaccgaaccaaacagcaaacctctgcactgatccacaatatatttgatagccactttgcggcgatccaga
tacatcatgacagtaattccaagagcgaagtcattcgtgatttctacactgatcgcgacacggatgtgctcaacttttt
cttcctcagtatcgaccaaagcgatccgtcgcacacaccagagttccgttttctaacggaccacaaaggcatcatttgg
gacgatggaaatgcgcatttctatggtgtgaacgaccttatccttgatagccttgccaatcgggtcagtttcagtaaca
actggtattacattaatgtcatgacctccattggttcgagacacatgctcgtgcgccgtgtgccgatcctagacccttc
aacaggagaggtgcttggtttctcatttaatgccgtcgtcttagacaacaacttcgctttgatggaaaagctcaagagt
gaaagtaacgtcgacaatgtggtgctggttgctaatagcgttcctttagcaaactctttgattggtgatgagccatata
acgttgctgatgtattgcagcgtaaaagttcagacaaaagactcgataagctgttggtaatagaaacgccaatcgtcgt
aaatgcagtgactaccgagctttgcttgttgacggtacaagacaatcagagtgtggtgacattacaaatccaacatatt
ctagccatgcttgcatcgatcatcggtatgatcatgattgccttaatgagtagggaatggattgagagtaaagtttcgg
cgcagttagaatctttgatgtcttacacccgctctgctcgtgaggaaaaagggtttgaacgatttggcggttcggatat
tgaagagtttgatcacatcggttcaacccttgaaagtacattcgaagagcttgaagcgcagaagaagtcgttccgagat
ctgtttaattttgccttatcacccatcatggtttggtctgaagagagtgtcctgattcagatgaaccctgccgcgcgca
aagaattagtgatcgaagacgatcatgaaatcatgcatccggtcttccaaggctttaaagagaaattgaccccacacct
caaaatggcggctcaaggtgcgacgttgactggggtgaacgtgcctattggtaataagatctaccgatggaacttgtcg
ccaattcgtgttgatggcgatatcagtggcattattgtgcaaggccaagacattacaacacttatcgaagccgagaagc
agagtaacattgcgcgtagagaagcagaaaaatcggcgcaagcacgtgcagacttccttgctaaaatgagccatgaaat
tcgtacgccaatcaacggcattttaggtgtcgcccaattattgaaagattctgtcgatacacaagagcagaagaatcaa
atcgacgtcctgtgccacagtggcgagcacttgcttgcagtactgaacgatattctcgatttctcaaagatagagcagg
gcaagttcaatattcagaaacacccgttctccttcaccgataccatgcgtacattggaaaatatttatcgtccgatttg
cacaaataagggggtggagttggtcatcgagaatgagcttgacccgaatgttgaaatcttcaccgatcaagtccgcttg
aatcagattctatttaacttagtgagtaatgccgttaagttcacgccgattggctcgattcgactgcacgcagaacttg
aacaattctatggtgcggagaacagcgtgttagttgtggaactgactgatactggcatcggcattgaaagcgataagct
cgaccaaatgttcgaaccttttgtgcaagaagagtcgacaaccacacgcgaatatggcggtagcggcctaggtttgacc
atcgttaagaacctagtcgatatgttagaaggtgatgttcaggtccgcagtagcaaggggggggggacaacatttgtta
taacacttccagtaaaagatcgtgagcgtgtcttaaggcctctggaggtcagtcaacgtatcaagccggaagccttgtt
tgatgaaagtttaaaagtgctactggtggaagataaccataccaatgcgtttatccttcaggctttctgtaagaagtat
aaaatgcaggtggattgggcgaaagatgggctggacgcgatggagctcctttctgataccacctacgatctgatcctca
tggataaccaattaccccaccttggtggtattgagaccacgcacgagattcgccagaacttgaggcttggaacgccaat
ttacgcgtgtacagcagacaccgcgaaagaaaccagtgatgcgtttatggcggcaggtgcaaactatgtcatgctgaag
ccaattaaagagaatgcgttacatgaggcgtttgtcgatttcaaacaacgtttcttggtagaaagaacctaacggttta
atggcagtgataagttaggggctgaagttaaataataaaagtaaagaagggagcgtaatgcactaatgccgttcactta
aggtgatcggcattgttttttctagggtatcggtactagagtccctatcagtgatagagattgacatccctatcagtga
tagagatactgagcac