BBa_K131015
1
LuxPQ
LuxPQ from Vibrio harveyi
2008-09-27T11:00:00Z
2015-05-08T01:09:47Z
GenBank Accession: NC_009784
Vibrio harveyi ATCC BAA-1116 chromosome II, REGION: 579654..580751.
Neiditch MB, Federle MJ, Pompeani AJ, Kelly RC, Swem DL, Jeffrey PD, Bassler BL, Hughson FM. Ligand-induced asymmetry in histidine sensor kinase complex regulates quorum sensing. Cell. 2006 Sep 22;126(6):1095-108.
Neiditch MB, Federle MJ, Pompeani AJ, Kelly RC, Swem DL, Jeffrey PD, Bassler BL, Hughson FM.
LuxPQ from Vibrio harveyi
LuxP is a ABC-type sugar transport system periplasmic protein (Protein ID = YP_001447483.1).
LuxQ is a histidine kinase (Protein ID = YP_001447484.1).
false
true
_259_
0
3190
9
It's complicated
true
LuxP and LuxQ are found in the operon, thus we decided to keep them together. We kept natural RBS in front of LuxP, as well as the intrinsic terminator. Silent mutations were performed on a EcoRI site located within LuxP, EcorI site within LuxQ, and XbaI site within LuxQ.
true
Thane Kubik
annotation1976844
1
Start of LuxP
range1976844
1
30
32
annotation1976850
1
LuxP
range1976850
1
30
1127
annotation1976854
1
Silent mutation to remove XbaI site
range1976854
1
1618
1618
annotation1976852
1
Silent mutation to remove EcoRI site
range1976852
1
599
599
annotation1976847
1
Stop for LuxQ
range1976847
1
3704
3706
annotation1976853
1
Silent mutation to remove EcoRI site
range1976853
1
1465
1465
annotation1976851
1
LuxQ
range1976851
1
1127
3706
annotation1976845
1
Stop for LuxP
range1976845
1
1125
1127
annotation1976846
1
Start of LuxQ
range1976846
1
1127
1129
BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986786
1
TetR 2
range1986786
1
26
44
annotation1986787
1
-10
range1986787
1
43
48
annotation1986783
1
TetR 1
range1986783
1
1
19
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986785
1
-35
range1986785
1
20
25
BBa_K131020
1
BBa_K131020
K131015+R0040
2008-10-27T12:00:00Z
2015-05-08T01:09:47Z
Vibrio harveyi genomic sequence
LuxPQ+R0040
false
false
_259_
0
3190
9
Not in stock
false
Intermediate part to construct AI-2 signaling circuit
false
Thane Kubik
component1990636
1
BBa_R0040
component1990635
1
BBa_K131015
annotation1990635
1
BBa_K131015
range1990635
1
1
3825
annotation1990636
1
BBa_R0040
range1990636
1
3834
3887
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_K131020_sequence
1
atgctcgataaaaactaaaagagcaatagatgaagaaagcgttactattttccctgatttctatggtcggtttttctccagcgtctcaagcaacacaagttttgaatgggtactggggttatcaagagtttttggacgagtttcccgagcaacgaaatctgaccaatgctttatcagaagcagtacgagcacagccggtcccactgtctaaaccgacacaacgcccgattaaaatatcagtggtttacccaggacagcaagtttcagattactgggtaagaaatattgcatcattcgaaaaacgtttgtataagttgaatattaactaccaactgaaccaagtgtttactcgtccaaatgctgatatcaagcaacaaagcttgtcattaatggaagcgctcaagagcaaatcggattacttgattttcacgcttgatacgacaagacaccgtaaatttgttgagcacgttttggactcaacgaacaccaaattgatcttgcaaaatatcactacaccagtccgtgagtgggacaaacatcaaccgtttttatatgtcggatttgaccacgcagaaggcagtcgtgaattagcaacagagttcggaaagttcttcccaaaacacacatattacagtgtgctctacttttctgaaggttatattagcgatgtgagaggtgatacttttattcaccaagtaaaccgtgataataactttgagctacaatcagcgtattacacgaaggcaaccaagcaatccggctatgatgctgcgaaagcgagtttagcaaaacatccagatgttgattttatctatgcatgttcgaccgacgtagcattaggtgcagtagacgcactggctgagttgggacgtgaagatattatgatcaatggctggggtggaggctctgctgagttagacgctatccagaagggtgatttagacatcaccgtcatgcgtatgaatgatgacactggcatagccatggcagaagcgattaagtgggacttggaagataaaccagttccgaccgtatactcaggtgactttgaaatcgtaacaaaggcagattcaccggagagaatcgaagcgctgaaaaagcgcgcatttagatattcagataattgatgacaacaacgcgatcaaacattaaaaagcgtcgctcgctggcgacgctcataacaaagatcatcattttagttcttgccccaattattctggggattttcattcagagctattacttctccaagcaaatcatttggcaagaagtagaccgaaccaaacagcaaacctctgcactgatccacaatatatttgatagccactttgcggcgatccagatacatcatgacagtaattccaagagcgaagtcattcgtgatttctacactgatcgcgacacggatgtgctcaactttttcttcctcagtatcgaccaaagcgatccgtcgcacacaccagagttccgttttctaacggaccacaaaggcatcatttgggacgatggaaatgcgcatttctatggtgtgaacgaccttatccttgatagccttgccaatcgggtcagtttcagtaacaactggtattacattaatgtcatgacctccattggttcgagacacatgctcgtgcgccgtgtgccgatcctagacccttcaacaggagaggtgcttggtttctcatttaatgccgtcgtcttagacaacaacttcgctttgatggaaaagctcaagagtgaaagtaacgtcgacaatgtggtgctggttgctaatagcgttcctttagcaaactctttgattggtgatgagccatataacgttgctgatgtattgcagcgtaaaagttcagacaaaagactcgataagctgttggtaatagaaacgccaatcgtcgtaaatgcagtgactaccgagctttgcttgttgacggtacaagacaatcagagtgtggtgacattacaaatccaacatattctagccatgcttgcatcgatcatcggtatgatcatgattgccttaatgagtagggaatggattgagagtaaagtttcggcgcagttagaatctttgatgtcttacacccgctctgctcgtgaggaaaaagggtttgaacgatttggcggttcggatattgaagagtttgatcacatcggttcaacccttgaaagtacattcgaagagcttgaagcgcagaagaagtcgttccgagatctgtttaattttgccttatcacccatcatggtttggtctgaagagagtgtcctgattcagatgaaccctgccgcgcgcaaagaattagtgatcgaagacgatcatgaaatcatgcatccggtcttccaaggctttaaagagaaattgaccccacacctcaaaatggcggctcaaggtgcgacgttgactggggtgaacgtgcctattggtaataagatctaccgatggaacttgtcgccaattcgtgttgatggcgatatcagtggcattattgtgcaaggccaagacattacaacacttatcgaagccgagaagcagagtaacattgcgcgtagagaagcagaaaaatcggcgcaagcacgtgcagacttccttgctaaaatgagccatgaaattcgtacgccaatcaacggcattttaggtgtcgcccaattattgaaagattctgtcgatacacaagagcagaagaatcaaatcgacgtcctgtgccacagtggcgagcacttgcttgcagtactgaacgatattctcgatttctcaaagatagagcagggcaagttcaatattcagaaacacccgttctccttcaccgataccatgcgtacattggaaaatatttatcgtccgatttgcacaaataagggggtggagttggtcatcgagaatgagcttgacccgaatgttgaaatcttcaccgatcaagtccgcttgaatcagattctatttaacttagtgagtaatgccgttaagttcacgccgattggctcgattcgactgcacgcagaacttgaacaattctatggtgcggagaacagcgtgttagttgtggaactgactgatactggcatcggcattgaaagcgataagctcgaccaaatgttcgaaccttttgtgcaagaagagtcgacaaccacacgcgaatatggcggtagcggcctaggtttgaccatcgttaagaacctagtcgatatgttagaaggtgatgttcaggtccgcagtagcaaggggggggggacaacatttgttataacacttccagtaaaagatcgtgagcgtgtcttaaggcctctggaggtcagtcaacgtatcaagccggaagccttgtttgatgaaagtttaaaagtgctactggtggaagataaccataccaatgcgtttatccttcaggctttctgtaagaagtataaaatgcaggtggattgggcgaaagatgggctggacgcgatggagctcctttctgataccacctacgatctgatcctcatggataaccaattaccccaccttggtggtattgagaccacgcacgagattcgccagaacttgaggcttggaacgccaatttacgcgtgtacagcagacaccgcgaaagaaaccagtgatgcgtttatggcggcaggtgcaaactatgtcatgctgaagccaattaaagagaatgcgttacatgaggcgtttgtcgatttcaaacaacgtttcttggtagaaagaacctaacggtttaatggcagtgataagttaggggctgaagttaaataataaaagtaaagaagggagcgtaatgcactaatgccgttcacttaaggtgatcggcattgttttttctagggtatcggtactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_K131015_sequence
1
atgctcgataaaaactaaaagagcaatagatgaagaaagcgttactattttccctgatttctatggtcggtttttctccagcgtctcaagcaacacaagttttgaatgggtactggggttatcaagagtttttggacgagtttcccgagcaacgaaatctgaccaatgctttatcagaagcagtacgagcacagccggtcccactgtctaaaccgacacaacgcccgattaaaatatcagtggtttacccaggacagcaagtttcagattactgggtaagaaatattgcatcattcgaaaaacgtttgtataagttgaatattaactaccaactgaaccaagtgtttactcgtccaaatgctgatatcaagcaacaaagcttgtcattaatggaagcgctcaagagcaaatcggattacttgattttcacgcttgatacgacaagacaccgtaaatttgttgagcacgttttggactcaacgaacaccaaattgatcttgcaaaatatcactacaccagtccgtgagtgggacaaacatcaaccgtttttatatgtcggatttgaccacgcagaaggcagtcgtgaattagcaacagagttcggaaagttcttcccaaaacacacatattacagtgtgctctacttttctgaaggttatattagcgatgtgagaggtgatacttttattcaccaagtaaaccgtgataataactttgagctacaatcagcgtattacacgaaggcaaccaagcaatccggctatgatgctgcgaaagcgagtttagcaaaacatccagatgttgattttatctatgcatgttcgaccgacgtagcattaggtgcagtagacgcactggctgagttgggacgtgaagatattatgatcaatggctggggtggaggctctgctgagttagacgctatccagaagggtgatttagacatcaccgtcatgcgtatgaatgatgacactggcatagccatggcagaagcgattaagtgggacttggaagataaaccagttccgaccgtatactcaggtgactttgaaatcgtaacaaaggcagattcaccggagagaatcgaagcgctgaaaaagcgcgcatttagatattcagataattgatgacaacaacgcgatcaaacattaaaaagcgtcgctcgctggcgacgctcataacaaagatcatcattttagttcttgccccaattattctggggattttcattcagagctattacttctccaagcaaatcatttggcaagaagtagaccgaaccaaacagcaaacctctgcactgatccacaatatatttgatagccactttgcggcgatccagatacatcatgacagtaattccaagagcgaagtcattcgtgatttctacactgatcgcgacacggatgtgctcaactttttcttcctcagtatcgaccaaagcgatccgtcgcacacaccagagttccgttttctaacggaccacaaaggcatcatttgggacgatggaaatgcgcatttctatggtgtgaacgaccttatccttgatagccttgccaatcgggtcagtttcagtaacaactggtattacattaatgtcatgacctccattggttcgagacacatgctcgtgcgccgtgtgccgatcctagacccttcaacaggagaggtgcttggtttctcatttaatgccgtcgtcttagacaacaacttcgctttgatggaaaagctcaagagtgaaagtaacgtcgacaatgtggtgctggttgctaatagcgttcctttagcaaactctttgattggtgatgagccatataacgttgctgatgtattgcagcgtaaaagttcagacaaaagactcgataagctgttggtaatagaaacgccaatcgtcgtaaatgcagtgactaccgagctttgcttgttgacggtacaagacaatcagagtgtggtgacattacaaatccaacatattctagccatgcttgcatcgatcatcggtatgatcatgattgccttaatgagtagggaatggattgagagtaaagtttcggcgcagttagaatctttgatgtcttacacccgctctgctcgtgaggaaaaagggtttgaacgatttggcggttcggatattgaagagtttgatcacatcggttcaacccttgaaagtacattcgaagagcttgaagcgcagaagaagtcgttccgagatctgtttaattttgccttatcacccatcatggtttggtctgaagagagtgtcctgattcagatgaaccctgccgcgcgcaaagaattagtgatcgaagacgatcatgaaatcatgcatccggtcttccaaggctttaaagagaaattgaccccacacctcaaaatggcggctcaaggtgcgacgttgactggggtgaacgtgcctattggtaataagatctaccgatggaacttgtcgccaattcgtgttgatggcgatatcagtggcattattgtgcaaggccaagacattacaacacttatcgaagccgagaagcagagtaacattgcgcgtagagaagcagaaaaatcggcgcaagcacgtgcagacttccttgctaaaatgagccatgaaattcgtacgccaatcaacggcattttaggtgtcgcccaattattgaaagattctgtcgatacacaagagcagaagaatcaaatcgacgtcctgtgccacagtggcgagcacttgcttgcagtactgaacgatattctcgatttctcaaagatagagcagggcaagttcaatattcagaaacacccgttctccttcaccgataccatgcgtacattggaaaatatttatcgtccgatttgcacaaataagggggtggagttggtcatcgagaatgagcttgacccgaatgttgaaatcttcaccgatcaagtccgcttgaatcagattctatttaacttagtgagtaatgccgttaagttcacgccgattggctcgattcgactgcacgcagaacttgaacaattctatggtgcggagaacagcgtgttagttgtggaactgactgatactggcatcggcattgaaagcgataagctcgaccaaatgttcgaaccttttgtgcaagaagagtcgacaaccacacgcgaatatggcggtagcggcctaggtttgaccatcgttaagaacctagtcgatatgttagaaggtgatgttcaggtccgcagtagcaaggggggggggacaacatttgttataacacttccagtaaaagatcgtgagcgtgtcttaaggcctctggaggtcagtcaacgtatcaagccggaagccttgtttgatgaaagtttaaaagtgctactggtggaagataaccataccaatgcgtttatccttcaggctttctgtaagaagtataaaatgcaggtggattgggcgaaagatgggctggacgcgatggagctcctttctgataccacctacgatctgatcctcatggataaccaattaccccaccttggtggtattgagaccacgcacgagattcgccagaacttgaggcttggaacgccaatttacgcgtgtacagcagacaccgcgaaagaaaccagtgatgcgtttatggcggcaggtgcaaactatgtcatgctgaagccaattaaagagaatgcgttacatgaggcgtttgtcgatttcaaacaacgtttcttggtagaaagaacctaacggtttaatggcagtgataagttaggggctgaagttaaataataaaagtaaagaagggagcgtaatgcactaatgccgttcacttaaggtgatcggcattgttttttctagggtatcgg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z