##gff-version 3
##sequence-region BBa_K131021 1 2007
BBa_K131021	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K131021	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K131021	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K131021	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K131021	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K131021	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K131021	.	engineered_region	63	2007	.	+	0	ID=BBa_K131016;Name=BBa_K131016
BBa_K131021	.	stop_codon	1793	1795	.	+	0	ID=annotation1976861;Name=Stop for LuxU;Parent=BBa_K131016
BBa_K131021	.	start_codon	1451	1453	.	+	0	ID=annotation1976860;Name=Start for LuxU;Parent=BBa_K131016
BBa_K131021	.	CDS	1451	1795	.	+	0	ID=annotation1976859;Name=LuxU;Parent=BBa_K131016
BBa_K131021	.	ribosome_entry_site	67	74	.	+	0	ID=annotation1976855;Name=RBS;Parent=BBa_K131016
BBa_K131021	.	start_codon	93	95	.	+	0	ID=annotation1976857;Name=Start for LuxO;Parent=BBa_K131016
BBa_K131021	.	stop_codon	1452	1454	.	+	0	ID=annotation1976858;Name=Stop for LuxO;Parent=BBa_K131016
BBa_K131021	.	CDS	93	1454	.	+	0	ID=annotation1976856;Name=LuxO;Parent=BBa_K131016
>BBa_K131021
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagacagaaggtcaaaagtc
tcgttatctacttatggtagaagacaccgcatccgttgcggcactttaccgctcttacctcacgccacttggcatcgat
atcaatattgttggaacaggcagagacgccattgaaagcctgaaccatcgcattcctgatcttattctgctcgatcttc
gtctacctgatatgacggggatggacgtattgcacgcggtgaagaaaagccacccagacgtgccaatcatcttcatgac
agcccatggttctatcgatactgcggtagaggcgatgcgccacggttctcaagacttcctaatcaaaccatgtgaagca
gaccgtttacgtgtcacggtgaacaatgcgatccgtaaagcaaccaaattaaagaatgaagctgacaaccccggtaacc
aaaattaccaaggcttcatcggcagtagccaaacgatgcagcaggtttaccgcaccattgactcggcagcgagcagtaa
agcgagtattttcatcacgggtgaaagtggtacgggtaaagaagtgtgtgccgaagcgattcacgcagcaagcaaacgc
ggtgataagccgtttatcgccatcaactgtgcggcaatcccgaaagaccttattgaaagtgagctgtttggtcacgtaa
aaggtgcgtttactggtgctgcgaatgaccgacaaggtgcggcagagcttgctgatggcggcaccttgttccttgatga
actctgtgaaatggacttggatcttcaaactaagctattgcgctttatccaaacgggtacattccaaaaagtcggttct
tctaaaatgaagagcgtggatgtgcgctttgtgtgtgcaactaaccgagacccttggaaagaagtgcaagaaggccgtt
tccgtgaagacttgtattaccgtttgtacgtgattcctttgcaccttccgccgctgcgtgagcgtggtaaagacgttat
tgaaattgcatactcgttgcttggttatatgtctcatgaggaaggtaagagtttcgtccgtttcgcacaagacgtgatt
gaaagattcaacagctacgaatggccgggtaacgttcgccagttgcaaaacgtattgcgtaatatcgtggtactgaaca
atggcaaagagatcacgctggatatgttaccgccaccactgaatcagcctgttgtgcgccaatcggtagcaaaattcat
tgaacctgacattatgacggtgtcagatattatgccgctttggatgacagagaaaatggctattgagcaggcaattcaa
gcgtgtgaaggcaacattccacgcgctgctggctatttggatgttagtccatcaacgatttatcgcaagttgcaagctt
ggaatagcaaggacgaaaaacaaaacgtatgaatacggacgtattaaatcagcaaaaaattgaagaactgtctgcggaa
attggtagcgataatgttcctgttttgcttgatatttttcttggggaaatggactcctacattggcactttaactgaac
ttcagggctcagagcagctgttgtatttaaaagagatcagccacgcactgaaaagtagtgctgccagctttggcgcaga
tcgattgtgtgaacgagcgattgccatcgacaagaaagcaaaagcgaatcaattgcaagagcaggggatggagacgagc
gaaatgctcgctttacttcatatcactcgtgacgcctaccgttcttggacaaactaacgtttcgagcaagacattaagc
gcaacgtaaaaacacaaagcccttccggtgtggaagggcttttttgtttggggagtttgctccgaatcgaagccgcttt
ctcaatgcttttcgtctagttagacagtaagcgctccataaaacccgcattctaatcgcctagcgcgaagaataagatc
aagtctccaaccatgaggagatttgaaatggg