BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986783
1
TetR 1
range1986783
1
1
19
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986786
1
TetR 2
range1986786
1
26
44
annotation1986785
1
-35
range1986785
1
20
25
annotation1986787
1
-10
range1986787
1
43
48
BBa_K131021
1
BBa_K131021
R0040+K131016
2008-10-27T12:00:00Z
2015-05-08T01:09:47Z
LuxOU from genomic sequence for Vibrio harveyi
R0040 controlling the expression of LuxOU
false
false
_259_
0
3190
9
Not in stock
false
LuxOU sequence contains intrinsic terminators
false
Thane Kubik
component1990641
1
BBa_R0040
component1990653
1
BBa_K131016
annotation1990641
1
BBa_R0040
range1990641
1
1
54
annotation1990653
1
BBa_K131016
range1990653
1
63
2007
BBa_K131016
1
LuxOU
LuxOU from Vibrio harveyi
2008-09-27T11:00:00Z
2015-05-08T01:09:47Z
GenBank Accession# L26221
LuxO Protein ID# AAD12736.1
LuxU Protein ID# AAD12737.2
Jeremy A. Freeman and Bonnie L. Bassler. Sequence and function of LuxU: a two-component phosphorelay protein that regulates quorum sensing in Vibrio harveyi. 1999. Journal of Bacteriology. Feb 1999, p. 899-906.
Vibrio harveyi repressor protein LuxO and phosphorelay protein LuxU genes. LuxO and LuxU are regulators of luminescence in Vibrio harveyi.
false
true
_259_
0
3190
9
It's complicated
true
LuxO and LuxU are found within the operon, therefore we have decided to keep these genes together. The natural RBS was also kept.
true
Thane Kubik
annotation1976860
1
Start for LuxU
range1976860
1
1389
1391
annotation1976858
1
Stop for LuxO
range1976858
1
1390
1392
annotation1976857
1
Start for LuxO
range1976857
1
31
33
annotation1976861
1
Stop for LuxU
range1976861
1
1731
1733
annotation1976859
1
LuxU
range1976859
1
1389
1733
annotation1976855
1
RBS
range1976855
1
5
12
annotation1976856
1
LuxO
range1976856
1
31
1392
BBa_K131016_sequence
1
acagaaggtcaaaagtctcgttatctacttatggtagaagacaccgcatccgttgcggcactttaccgctcttacctcacgccacttggcatcgatatcaatattgttggaacaggcagagacgccattgaaagcctgaaccatcgcattcctgatcttattctgctcgatcttcgtctacctgatatgacggggatggacgtattgcacgcggtgaagaaaagccacccagacgtgccaatcatcttcatgacagcccatggttctatcgatactgcggtagaggcgatgcgccacggttctcaagacttcctaatcaaaccatgtgaagcagaccgtttacgtgtcacggtgaacaatgcgatccgtaaagcaaccaaattaaagaatgaagctgacaaccccggtaaccaaaattaccaaggcttcatcggcagtagccaaacgatgcagcaggtttaccgcaccattgactcggcagcgagcagtaaagcgagtattttcatcacgggtgaaagtggtacgggtaaagaagtgtgtgccgaagcgattcacgcagcaagcaaacgcggtgataagccgtttatcgccatcaactgtgcggcaatcccgaaagaccttattgaaagtgagctgtttggtcacgtaaaaggtgcgtttactggtgctgcgaatgaccgacaaggtgcggcagagcttgctgatggcggcaccttgttccttgatgaactctgtgaaatggacttggatcttcaaactaagctattgcgctttatccaaacgggtacattccaaaaagtcggttcttctaaaatgaagagcgtggatgtgcgctttgtgtgtgcaactaaccgagacccttggaaagaagtgcaagaaggccgtttccgtgaagacttgtattaccgtttgtacgtgattcctttgcaccttccgccgctgcgtgagcgtggtaaagacgttattgaaattgcatactcgttgcttggttatatgtctcatgaggaaggtaagagtttcgtccgtttcgcacaagacgtgattgaaagattcaacagctacgaatggccgggtaacgttcgccagttgcaaaacgtattgcgtaatatcgtggtactgaacaatggcaaagagatcacgctggatatgttaccgccaccactgaatcagcctgttgtgcgccaatcggtagcaaaattcattgaacctgacattatgacggtgtcagatattatgccgctttggatgacagagaaaatggctattgagcaggcaattcaagcgtgtgaaggcaacattccacgcgctgctggctatttggatgttagtccatcaacgatttatcgcaagttgcaagcttggaatagcaaggacgaaaaacaaaacgtatgaatacggacgtattaaatcagcaaaaaattgaagaactgtctgcggaaattggtagcgataatgttcctgttttgcttgatatttttcttggggaaatggactcctacattggcactttaactgaacttcagggctcagagcagctgttgtatttaaaagagatcagccacgcactgaaaagtagtgctgccagctttggcgcagatcgattgtgtgaacgagcgattgccatcgacaagaaagcaaaagcgaatcaattgcaagagcaggggatggagacgagcgaaatgctcgctttacttcatatcactcgtgacgcctaccgttcttggacaaactaacgtttcgagcaagacattaagcgcaacgtaaaaacacaaagcccttccggtgtggaagggcttttttgtttggggagtttgctccgaatcgaagccgctttctcaatgcttttcgtctagttagacagtaagcgctccataaaacccgcattctaatcgcctagcgcgaagaataagatcaagtctccaaccatgaggagatttgaaatggg
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_K131021_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagacagaaggtcaaaagtctcgttatctacttatggtagaagacaccgcatccgttgcggcactttaccgctcttacctcacgccacttggcatcgatatcaatattgttggaacaggcagagacgccattgaaagcctgaaccatcgcattcctgatcttattctgctcgatcttcgtctacctgatatgacggggatggacgtattgcacgcggtgaagaaaagccacccagacgtgccaatcatcttcatgacagcccatggttctatcgatactgcggtagaggcgatgcgccacggttctcaagacttcctaatcaaaccatgtgaagcagaccgtttacgtgtcacggtgaacaatgcgatccgtaaagcaaccaaattaaagaatgaagctgacaaccccggtaaccaaaattaccaaggcttcatcggcagtagccaaacgatgcagcaggtttaccgcaccattgactcggcagcgagcagtaaagcgagtattttcatcacgggtgaaagtggtacgggtaaagaagtgtgtgccgaagcgattcacgcagcaagcaaacgcggtgataagccgtttatcgccatcaactgtgcggcaatcccgaaagaccttattgaaagtgagctgtttggtcacgtaaaaggtgcgtttactggtgctgcgaatgaccgacaaggtgcggcagagcttgctgatggcggcaccttgttccttgatgaactctgtgaaatggacttggatcttcaaactaagctattgcgctttatccaaacgggtacattccaaaaagtcggttcttctaaaatgaagagcgtggatgtgcgctttgtgtgtgcaactaaccgagacccttggaaagaagtgcaagaaggccgtttccgtgaagacttgtattaccgtttgtacgtgattcctttgcaccttccgccgctgcgtgagcgtggtaaagacgttattgaaattgcatactcgttgcttggttatatgtctcatgaggaaggtaagagtttcgtccgtttcgcacaagacgtgattgaaagattcaacagctacgaatggccgggtaacgttcgccagttgcaaaacgtattgcgtaatatcgtggtactgaacaatggcaaagagatcacgctggatatgttaccgccaccactgaatcagcctgttgtgcgccaatcggtagcaaaattcattgaacctgacattatgacggtgtcagatattatgccgctttggatgacagagaaaatggctattgagcaggcaattcaagcgtgtgaaggcaacattccacgcgctgctggctatttggatgttagtccatcaacgatttatcgcaagttgcaagcttggaatagcaaggacgaaaaacaaaacgtatgaatacggacgtattaaatcagcaaaaaattgaagaactgtctgcggaaattggtagcgataatgttcctgttttgcttgatatttttcttggggaaatggactcctacattggcactttaactgaacttcagggctcagagcagctgttgtatttaaaagagatcagccacgcactgaaaagtagtgctgccagctttggcgcagatcgattgtgtgaacgagcgattgccatcgacaagaaagcaaaagcgaatcaattgcaagagcaggggatggagacgagcgaaatgctcgctttacttcatatcactcgtgacgcctaccgttcttggacaaactaacgtttcgagcaagacattaagcgcaacgtaaaaacacaaagcccttccggtgtggaagggcttttttgtttggggagtttgctccgaatcgaagccgctttctcaatgcttttcgtctagttagacagtaagcgctccataaaacccgcattctaatcgcctagcgcgaagaataagatcaagtctccaaccatgaggagatttgaaatggg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z