LOCUS       BBa_K131025             1442 bp    DNA     linear       10-Jun-2024
DEFINITION  J13002+K131023
ACCESSION   BBa_K131025
VERSION     BBa_K131025.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    219..221
                     /label=D47A
     misc_feature    81..83
                     /label=Start
     misc_feature    1440..1442
                     /label=Stop
     CDS             81..1442
                     /label=BBa_K131023
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtagaag acaccgcatc cgttgcggca ctttaccgct
      121 cttacctcac gccacttggc atcgatatca atattgttgg aacaggcaga gacgccattg
      181 aaagcctgaa ccatcgcatt cctgatctta ttctgctcgc gctccgtcta cctgatatga
      241 cggggatgga cgtattgcac gcggtgaaga aaagccaccc agacgtgcca atcatcttca
      301 tgacagccca tggttctatc gatactgcgg tagaggcgat gcgccacggt tctcaagact
      361 tcctaatcaa accatgtgaa gcagaccgtt tacgtgtcac ggtgaacaat gcgatccgta
      421 aagcaaccaa attaaagaat gaagctgaca accccggtaa ccaaaattac caaggcttca
      481 tcggcagtag ccaaacgatg cagcaggttt accgcaccat tgactcggca gcgagcagta
      541 aagcgagtat tttcatcacg ggtgaaagtg gtacgggtaa agaagtgtgt gccgaagcga
      601 ttcacgcagc aagcaaacgc ggtgataagc cgtttatcgc catcaactgt gcggcaatcc
      661 cgaaagacct tattgaaagt gagctgtttg gtcacgtaaa aggtgcgttt actggtgctg
      721 cgaatgaccg acaaggtgcg gcagagcttg ctgatggcgg caccttgttc cttgatgaac
      781 tctgtgaaat ggacttggat cttcaaacta agctattgcg ctttatccaa acgggtacat
      841 tccaaaaagt cggttcttct aaaatgaaga gcgtggatgt gcgctttgtg tgtgcaacta
      901 accgagaccc ttggaaagaa gtgcaagaag gccgtttccg tgaagacttg tattaccgtt
      961 tgtacgtgat tcctttgcac cttccgccgc tgcgtgagcg tggtaaagac gttattgaaa
     1021 ttgcatactc gttgcttggt tatatgtctc atgaggaagg taagagtttc gtccgtttcg
     1081 cacaagacgt gattgaaaga ttcaacagct acgaatggcc gggtaacgtt cgccagttgc
     1141 aaaacgtatt gcgtaatatc gtggtactga acaatggcaa agagatcacg ctggatatgt
     1201 taccgccacc actgaatcag cctgttgtgc gccaatcggt agcaaaattc attgaacctg
     1261 acattatgac ggtgtcagat attatgccgc tttggatgac agagaaaatg gctattgagc
     1321 aggcaattca agcgtgtgaa ggcaacattc cacgcgctgc tggctatttg gatgttagtc
     1381 catcaacgat ttatcgcaag ttgcaagctt ggaatagcaa ggacgaaaaa caaaacgtat
     1441 ga
//