BBa_K1311003 1 BBa_K1311003 promoterK081005+TALE-N_terminal-LacZ-C_terminal+terminatorB0015 2014-10-08T11:00:00Z 2015-05-08T01:09:47Z natural TALE sequence Our team use this part as a final vector for TALE(TranscriptionActivator???Like Effectors, TALE) assembly via Golden Gate Assembly. When we insert the TALE repeats into this vector, the reporter gene LacZ will be knocked out by the restriction enzyme Esp3I. Then we can use blue-white screening to select the right white colonies by add 40μL of 20mg/mL X-gal +40μL of 0.1M IPTG into the plate. false false _1686_ 0 20377 9 It's complicated true The N-terminal of TALE contains the restriction sites of PstI. Therefore, users should note if they want to use PstI to digest this plasmid. false Xinye Wang annotation2407893 1 BBa_K081005 range2407893 1 1 58 annotation2407892 1 BBa_J23100 range2407892 1 1 35 annotation2407894 1 RBS-1\Strong range2407894 1 44 58 annotation2407907 1 N-terminal range2407907 1 65 893 annotation2407916 1 lacZ_promoter range2407916 1 1273 1302 annotation2407895 1 RBS range2407895 1 51 55 annotation2407915 1 Reporter_LacZ range2407915 1 952 1112 annotation2407917 1 C-terminal range2407917 1 1380 2165 BBa_K1311003_sequence 1 atttctggaattcgcggccgcttctagagttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatggatcccattcgtccgcgcaggccaagtcctgcccgcgagcttctgcccggaccccaaccggatagggttcagccgactgcagatcgtggggtgtctgcgcctgctggcagccctctggatggcttgcccgctcggcggacggtgtcccggacccggctgccatctccccctgcgccctcacctgcgttctcggcgggcagcttcagcgatctgctccgtccgttcgatccgtcgcttcttgatacatcgcttcttgattcgatgcctgccgtcggcacgccgcatacagcggctgccccagcagagtgggatgaggcgcaatcggctctgcgtgcagccgatgacccgccacccaccgtgcgtgtcgctgtcactgccgcgcggccgccgcgcgccaagccggccccgcgacggcgtgctgcgcaaccctccgacgcttcgccggccgcgcaggtggatctacgcacgctcggctacagtcagcagcagcaagagaagatcaaaccgaaggtgcgttcgacagtggcgcagcaccacgaggcactggtgggccatgggtttacacacgcgcacatcgttgcgctcagccaacacccggcagcgttagggaccgtcgctgtcacgtatcagcacataatcacggcgttgccagaggcgacacacgaagacatcgttggcgtcggcaaacagtggtccggcgcacgcgccctggaggccttgctcacggatgcgggggagttgagaggtccgccgttacagttggacacaggccaacttgtgaagattgcaaaacgtggcggcgtgaccgcaatggaggcagtgcatgcatcgcgcaatgcactgacgggtgcccccctggagacgggcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgagcgcgcgtaatacgactcactatagggcgaattgggtaccgggccccccctcgaggtcctccagcttttgttccctttagtgagggttaattgcgcgcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccaccggtcgtctccaacgaccacctcgtcgccttggcctgcctcggcggacgtcctgccatggatgcagtgaaaaagggattgccgcacgcgccggaattgatcagaagagtcaatcgccgtattggcgaacgcacgtcccatcgcgttgccgactacgcgcaagtggttcgcgtgctggagtttttccagtgccactcccacccagcgtacgcatttgatgaggccatgacgcagttcgggatgagcaggaacgggttggtacagctctttcgcagagtgggcgtcaccgaactcgaagcccgcggtggaacgctccccccagcctcgcagcgttgggaccgtatcctccaggcatcagggatgaaaagggccaaaccgtcccctacttcagctcaaacaccggatcaggcgtctttgcatgcattcgccgattcgctggagcgtgaccttgatgcgcccagcccaatgcacgagggagatcagacgcgggcaagcagccgtaaacggtcccgatcggatcgtgctgtcaccggcccctccgcacagcaggctgtcgaggtgcgcgttcccgaacagcgcgatgcgctgcatttgcccctcagctggagggtaaaacgcccgcgtaccaggatctggggcggcctcccggatcctggtacgcccatggctgccgacctggcagcgtccagcaccgtgatgtgggaacaagatgcggaccccttcgcaggggcagcggatgatttcccggcattcaacgaagaggaactcgcatggttgatggagctattgcctcagtgaaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z