LOCUS       BBa_K1311004            3196 bp    DNA     linear       16-Feb-2025
DEFINITION  TetR inducible promoter+TALE N-terminal +LacZ+TALE C-terminal
ACCESSION   BBa_K1311004
VERSION     BBa_K1311004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..35
                     /label=J23101
     misc_feature    70..689
                     /label=TetR(C0040)
     RBS             44..58
                     /label=RBS-strong (B0030)
     misc_feature    897..915
                     /label=TetR1
     misc_feature    922..941
                     /label=TetR2
     misc_feature    957..1785
                     /label=TALE N-terminal
     misc_feature    1844..2004
                     /label=Reporter LacZ
     promoter        2165..2194
                     /label=lac promoter
     misc_feature    2272..3057
                     /label=TALE C-terminal
     promoter        938..944
                     /label=Promoter
     misc_feature    760..888
                     /label=B0015
     misc_feature    3068..3196
                     /label=B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagattaaag aggagaaata
       61 ctagagatgt ccagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat
      121 gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag
      181 cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag
      241 atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt
      301 ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa
      361 gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc
      421 tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg
      481 cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg
      541 gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat
      601 caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa
      661 aaacaactta aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc tttagtagct
      721 taataacact gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa
      781 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
      841 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtccc
      901 tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac tactagatgg
      961 atcccattcg tccgcgcagg ccaagtcctg cccgcgagct tctgcccgga ccccaaccgg
     1021 atagggttca gccgactgca gatcgtgggg tgtctgcgcc tgctggcagc cctctggatg
     1081 gcttgcccgc tcggcggacg gtgtcccgga cccggctgcc atctccccct gcgccctcac
     1141 ctgcgttctc ggcgggcagc ttcagcgatc tgctccgtcc gttcgatccg tcgcttcttg
     1201 atacatcgct tcttgattcg atgcctgccg tcggcacgcc gcatacagcg gctgccccag
     1261 cagagtggga tgaggcgcaa tcggctctgc gtgcagccga tgacccgcca cccaccgtgc
     1321 gtgtcgctgt cactgccgcg cggccgccgc gcgccaagcc ggccccgcga cggcgtgctg
     1381 cgcaaccctc cgacgcttcg ccggccgcgc aggtggatct acgcacgctc ggctacagtc
     1441 agcagcagca agagaagatc aaaccgaagg tgcgttcgac agtggcgcag caccacgagg
     1501 cactggtggg ccatgggttt acacacgcgc acatcgttgc gctcagccaa cacccggcag
     1561 cgttagggac cgtcgctgtc acgtatcagc acataatcac ggcgttgcca gaggcgacac
     1621 acgaagacat cgttggcgtc ggcaaacagt ggtccggcgc acgcgccctg gaggccttgc
     1681 tcacggatgc gggggagttg agaggtccgc cgttacagtt ggacacaggc caacttgtga
     1741 agattgcaaa acgtggcggc gtgaccgcaa tggaggcagt gcatgcatcg cgcaatgcac
     1801 tgacgggtgc ccccctggag acgggcgccg ctacagggcg cgtcccattc gccattcagg
     1861 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     1921 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     1981 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt
     2041 accgggcccc ccctcgaggt cctccagctt ttgttccctt tagtgagggt taattgcgcg
     2101 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     2161 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     2221 actcacatta attgcgttgc gctcactgcc cgctttccac cggtcgtctc caacgaccac
     2281 ctcgtcgcct tggcctgcct cggcggacgt cctgccatgg atgcagtgaa aaagggattg
     2341 ccgcacgcgc cggaattgat cagaagagtc aatcgccgta ttggcgaacg cacgtcccat
     2401 cgcgttgccg actacgcgca agtggttcgc gtgctggagt ttttccagtg ccactcccac
     2461 ccagcgtacg catttgatga ggccatgacg cagttcggga tgagcaggaa cgggttggta
     2521 cagctctttc gcagagtggg cgtcaccgaa ctcgaagccc gcggtggaac gctcccccca
     2581 gcctcgcagc gttgggaccg tatcctccag gcatcaggga tgaaaagggc caaaccgtcc
     2641 cctacttcag ctcaaacacc ggatcaggcg tctttgcatg cattcgccga ttcgctggag
     2701 cgtgaccttg atgcgcccag cccaatgcac gagggagatc agacgcgggc aagcagccgt
     2761 aaacggtccc gatcggatcg tgctgtcacc ggcccctccg cacagcaggc tgtcgaggtg
     2821 cgcgttcccg aacagcgcga tgcgctgcat ttgcccctca gctggagggt aaaacgcccg
     2881 cgtaccagga tctggggcgg cctcccggat cctggtacgc ccatggctgc cgacctggca
     2941 gcgtccagca ccgtgatgtg ggaacaagat gcggacccct tcgcaggggc agcggatgat
     3001 ttcccggcat tcaacgaaga ggaactcgca tggttgatgg agctattgcc tcagtgaaat
     3061 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     3121 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     3181 cctttctgcg tttata
//