LOCUS       BBa_K1311007            2412 bp    DNA     linear       26-Apr-2024
DEFINITION  A reporter for the TALE protein binding ability
ACCESSION   BBa_K1311007
VERSION     BBa_K1311007.1  
FEATURES             Location/Qualifiers
     misc_feature    1..35
                     /label=BBa_J23102
     misc_feature    42..128
                     /label=TALE binding site+RBS
     misc_feature    135..1169
                     /label=BBa_C0012
     CDS             135..1169
                     /label=lacI-LVA
     misc_feature    1203..1331
                     /label=BBa_B0015
     stem_loop       1214..1257
                     /label=stem_loop
     polyA_site      1318..1331
                     /label=polya
     misc_feature    1324..1324
                     /label=stop
     misc_feature    1340..2408
                     /label=BBa_J04450
     misc_feature    1340..1539
                     /label=BBa_R0010
     misc_feature    1428..1465
                     /label=CAP
     promoter        1476..1481
                     /label=-35
     promoter        1500..1505
                     /label=-10
     misc_feature    1505..1539
                     /label=LacI
     misc_feature    1512..1512
                     /label=start
     misc_feature    1548..1559
                     /label=BBa_B0034
     misc_feature    1552..1555
                     /label=conserved
     misc_feature    1566..2246
                     /label=BBa_E1010
     CDS             1566..2240
                     /label=mrfp1
     misc_feature    2280..2359
                     /label=BBa_B0010
     stem_loop       2291..2334
                     /label=stem_loop
     misc_feature    2368..2408
                     /label=BBa_B0012
     stem_loop       2375..2394
                     /label=T7 TE
     polyA_site      2395..2408
                     /label=polya
     misc_feature    2401..2401
                     /label=stop
ORIGIN
        1 gacagctagc tcagtcctag gtactgtgct agctactaga gaaagagggg acaaacgtac
       61 ctcatcagga acatgttagt gacctcatca ggaacatgtt agtgacctca tcaggaacat
      121 gttagtacta gagatgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc
      181 ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga
      241 aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact
      301 ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc
      361 gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt
      421 gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc
      481 gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt
      541 ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat
      601 caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc
      661 attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct
      721 gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg
      781 ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg
      841 catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc
      901 cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac
      961 cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct
     1021 ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa
     1081 tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac
     1141 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     1201 ggaaagcggg cagtaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1261 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1321 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagcaatac gcaaaccgcc
     1381 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     1441 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     1501 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     1561 cacaaaagag gagaaaatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa
     1621 agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg
     1681 tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc
     1741 gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca
     1801 cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg
     1861 tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga
     1921 cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt
     1981 tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg
     2041 tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc
     2101 tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac
     2161 cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga
     2221 acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgataagtgc tagtgtagat
     2281 cgcccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     2341 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     2401 tctgcgttta ta
//